Ciliary dyskinesia is an early feature of respiratory syncytial virus infection
Open accessAbstract
Respiratory syncytial virus is a major cause of respiratory disease. There are conflicting accounts of the response of human epithelial cells to respiratory syncytial virus and a lack of data on its effect on ciliary function. Our aim was to study the early stages of respiratory syncytial virus infection of primary human basal and ciliated cultures. Using high speed videomicroscopy, we found that ciliary beat frequency was unaffected by respiratory syncytial virus infection over 72 h; however, ciliary dyskinesia significantly increased within 24 h of infection (p<0.05). Transmission electron microscopy revealed that ultrastructural abnormalities were confined to ciliated cells, including increased cilia loss and mitochondrial damage. Confocal immunofluorescence microscopy showed that respiratory syncytial virus antigen gradually spread from the cell surface to the ciliary tip of infected cells over 3 days. Interestingly, ciliated cultures secreted fewer viruses than basal (progenitor) cell cultures and produced a chemokine response focused on recruitment of neutrophils. This study highlights differences in infection models and underscores the need to explore further the role of ciliated cells in the establishment of respiratory syncytial virus infection. Increased ciliary dyskinesia combined with ciliary loss and epithelial damage is likely to result in reduced mucociliary clearance early in the infective process.
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Viral infections can reduce ciliary beat frequency and the number of ciliated cells, disrupt the coordinated movement of cilia, and impede the repair of respiratory epithelial cells, further leading to reduced mucociliary clearance.
“Second, viral infections can also reduce ciliary beat frequency and the number of ciliated cells, disrupt the coordinated movement of cilia, and impede the repair of respiratory epithelial cells, further leading to reduced mucociliary clearance (126, 127).”
From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [127]
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References 29 · Cited by 103
- Authors
- Smith, C. M., Kulkarni, H., Radhakrishnan, P., Rutman, A., Bankart, M. J., Williams, G., Hirst, R. A., Easton, A. J., Andrew, P. W., & O’Callaghan, C.
- Affiliations
- University of Leicester (GB)
- Keele University (GB)
- University of Warwick (GB)
- Venue
- European Respiratory Journal
- Publisher
- European Respiratory Society (ERS)
- Published
- 2013-03-21
- Volume
- 43
- Issue
- 2
- Pages
- 485-496
- ISSN
- 0903-1936 (ISSN-L)
- 1399-3003
- OpenAlex
- W2125555721
- Cited by
- 103
- References
- 29
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 6 · 2023: 11 · 2022: 10 · 2021: 6 · 2020: 12 · 2019: 12 · 2018: 6 · 2017: 8 · 2016: 7 · 2015: 2 · 2014: 7 · 2013: 2
- FWCI
- 4.37
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- European Respiratory Journal (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Respiratory viral infections research
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Keywords
- respiratory syncytial virus, ciliary dyskinesia, ciliary beat frequency, ciliated epithelial cells, basal cells, ciliary ultrastructure, mitochondrial damage, viral antigen distribution, mucociliary clearance, viral pathogenesis
- MeSH terms
- Adult, Antigens, Viral, Cells, Cultured, Cilia, Ciliary Motility Disorders, Epithelial Cells, Humans, Middle Aged, Mucociliary Clearance, Respiratory Syncytial Viruses, Respiratory Syncytial Virus Infections, Th1 Cells, Microscopy, Video, Young Adult
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