Peripheral muscle mitophagy is enhanced in patients with COPD
Abstract
Objective: COPD is hallmarked by a loss of skeletal muscle oxidative capacity which is already present in less advanced disease stages. In this study, we hypothesized that mitophagy (selective mitochondrial autophagy) is enhanced in skeletal muscle of COPD patients, and is associated with the loss of muscle oxidative capacity. Methods:Vastus lateralis biopsies were taken of COPD patients with mild-to-moderate airflow obstruction (FEV1 %pred=57.2(46.8,68.2), n=29) and healthy controls (FEV1 %pred=112.8(107.5;125.5), n=15). Oxidative capacity was assessed by protein quantification of multiple subunits of oxidative phosphorylation (OXPHOS) complexes. General autophagy- (LC3B, P62) and mitophagy- (FUNDC1, PINK1, Parkin, BNIP3L) associated protein and mRNA levels were quantified. Results: The protein levels of the majority of OXPHOS subunits were decreased in COPD patients. Mitophagy-associated protein levels increased (Parkin) or decreased (FUNDC1 & PINK1) in COPD patients, while mRNA expression (FUNDC1, PINK1, BNIP3L) was not different. In contrast, autophagy-associated protein and mRNA levels were unchanged in these patients. No inverse correlations were found between any of the mitophagy and oxidative capacity markers. Conclusions: This study shows changes in mitophagy expression profiles indicative of activated mitophagy in COPD patients with loss of muscle oxidative capacity. The observed mitophagy expression profile indicates an enhanced clearance of damaged mitochondria in…
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In COPD patients' quadriceps biopsies, FUNDC1 and PINK1 are decreased at the protein level, Parkin is increased, and no change is observed at the mRNA level.
“The same authors simultaneously investigated COPD patients’ quadriceps biopsies and found decreased FUNDC1 and PINK1 at the protein level, whereas Parkin was increased. Moreover, no change was observed at the mRNA level [109].”
From Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting (2023) · cites this paper as [109]
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Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting
Henrot, P., Dupin, I., Schilfarth, P., Esteves, P., Blervaque, L., Zysman, M., Gouzi, F., Hayot, M., Pomiès, P., & Berger, P. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24076454 · Cited by 85 · see citing works
“Moreover, no change was observed at the mRNA level [109].”
Background
- Authors
- Leermakers, P., Schols, A., van den Borst, B., de Theije, C., Kelders, M., & Gosker, H.
- Affiliations
- Maastricht University Medical Centre (NL)
- Venue
- 3.1 Molecular Pathology and Functional Genomics
- Publisher
- European Respiratory Society
- Published
- 2016-09
- Pages
- PA3408
- OpenAlex
- W2556209815
- Cited by
- 1
- References
- 0
- Citations by year
- 2023: 1
- Type
- conference abstract
- Language
- en
- Open access
- closed
- Topics
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
- Keywords
- chronic obstructive pulmonary disease, mitophagy, skeletal muscle, oxidative capacity, oxidative phosphorylation, Parkin, FUNDC1, PINK1, BNIP3L, autophagy, vastus lateralis
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