Proposed methods for testing and selecting the ERCC external RNA controls
Open accessAbstract
The External RNA Control Consortium (ERCC) is an ad-hoc group with approximately 70 members from private, public, and academic organizations. The group is developing a set of external RNA control transcripts that can be used to assess technical performance in gene expression assays. The ERCC is now initiating the Testing Phase of the project, during which candidate external RNA controls will be evaluated in both microarray and QRT-PCR gene expression platforms. This document describes the proposed experiments and informatics process that will be followed to test and qualify individual controls. The ERCC is distributing this description of the proposed testing process in an effort to gain consensus and to encourage feedback from the scientific community. On October 4–5, 2005, the ERCC met to further review the document, clarify ambiguities, and plan next steps. A summary of this meeting and changes to the test plan are provided as an appendix to this manuscript.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The ERCC spike-in collection contains 92 RNA species of different lengths and GC content, mixed at known concentrations, and covers 22 abundance levels separated by one fold change.
“The standardized ERCC (External RNA Controls Consortium)5 spike-in collections consist of a set of 92 RNA molecule species of varying lengths and GC contents, mixed at known concentrations, and represent 22 abundance levels that are spaced one fold change apart from each other (Supplementary Figure 1).”
From Power analysis of single-cell RNA-sequencing experiments (2017) · cites this paper as [5]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 13 · Cited by 187
Research works printed but not linked: 1
- Applied Biosystems ABI Prism 7770 Sequence Detection System User Bulletin #2
- Authors
- External RNA Controls Consortium
- Corresponding author
- External RNA Controls Consortium
- Venue
- BMC Genomics
- Published
- 2005-11-02
- Volume
- 6
- Issue
- 1
- Pages
- 150-150
- ISSN
- 1471-2164 (ISSN-L)
- PMC
- PMC1325234
- OpenAlex
- W107998980
- Cited by
- 187
- References
- 13
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 14 · 2023: 4 · 2022: 5 · 2021: 12 · 2020: 16 · 2019: 15 · 2018: 11 · 2017: 10 · 2016: 11 · 2015: 9 · 2014: 11 · 2013: 8 · 2012: 2
- FWCI
- 3.15
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- BMC Genomics (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Apollo (University of Cambridge) (submittedVersion, cc-by)
- Apollo (cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- MeSH terms
- Bacteria, DNA, Dose-Response Relationship, Drug, Genetic Techniques, Humans, Nucleic Acid Hybridization, Plasmids, Reference Standards, RNA, RNA, Messenger, Models, Statistical, DNA Primers, Computational Biology, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Genomics
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
