The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Extensive exometabolome analysis reveals extended overflow metabolism in various microorganisms
Open accessAbstract
Overflow metabolism is well known for yeast, bacteria and mammalian cells. It typically occurs under glucose excess conditions and is characterized by excretions of by-products such as ethanol, acetate or lactate. This phenomenon, also denoted the short-term Crabtree effect, has been extensively studied over the past few decades, however, its basic regulatory mechanism and functional role in metabolism is still unknown. Here we present a comprehensive quantitative and time-dependent analysis of the exometabolome of Escherichia coli, Corynebacterium glutamicum, Bacillus licheniformis, and Saccharomyces cerevisiae during well-controlled bioreactor cultivations. Most surprisingly, in all cases a great diversity of central metabolic intermediates and amino acids is found in the culture medium with extracellular concentrations varying in the micromolar range. Different hypotheses for these observations are formulated and experimentally tested. As a result, the intermediates in the culture medium during batch growth must originate from passive or active transportation due to a new phenomenon termed “extended” overflow metabolism. Moreover, we provide broad evidence that this could be a common feature of all microorganism species when cultivated under conditions of carbon excess and non-inhibited carbon uptake. In turn, this finding has consequences for metabolite balancing and, particularly, for intracellular metabolite quantification and 13 C-metabolic flux analysis.
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Self-citationPyruvate Metabolism inSaccharomyces cerevisiae
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Advances in Biochemical Engineering
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Dynamic modeling of the central carbon metabolism of Escherichia coli
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How Saccharomyces Responds to Nutrients
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Metabolic dependencies drive species co-occurrence in diverse microbial communities
Zelezniak, A., Andrejev, S., Ponomarova, O., Mende, D. R., Bork, P., & Patil, K. R. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1421834112 · Cited by 930
Yeast Creates a Niche for Symbiotic Lactic Acid Bacteria through Nitrogen Overflow
Ponomarova, O., Gabrielli, N., Sévin, D. C., Mülleder, M., Zirngibl, K., Bulyha, K., Andrejev, S., Kafkia, E., Typas, A., Sauer, U., et al. (2017) · Cell Systems · doi:10.1016/j.cels.2017.09.002 · Cited by 409
Trophic Interactions and the Drivers of Microbial Community Assembly
Gralka, M., Szabo, R., Stocker, R., & Cordero, O. X. (2020) · Current Biology · doi:10.1016/j.cub.2020.08.007 · Cited by 331
Costless metabolic secretions as drivers of interspecies interactions in microbial ecosystems
Pacheco, A. R., Moel, M., & Segrè, D. (2019) · Nature Communications · doi:10.1038/s41467-018-07946-9 · Cited by 328
Morschett, H., Schiprowski, D., Rohde, J., Wiechert, W., & Oldiges, M. (2017) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-016-1731-5 · Cited by 11
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Metadata
- Authors
- Paczia, N., Nilgen, A., Lehmann, T., Gätgens, J., Wiechert, W., & Noack, S.
- Venue
- Microbial Cell Factories
- Published
- 2012-09-11
- Volume
- 11
- Issue
- 1
- Pages
- 122-122
- ISSN
- 1475-2859 (ISSN-L)
- OpenAlex
- W2131223372
- Cited by
- 295
- References
- 33
- FWCI
- 6.04
- Type
- journal article
- Language
- en
- Open access
- gold
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- Paczia, N., Nilgen, A., Lehmann, T., Gätgens, J., Wiechert, W., & Noack, S. (2012). Extensive exometabolome analysis reveals extended overflow metabolism in various microorganisms. Microbial Cell Factories. https://doi.org/10.1186/1475-2859-11-122
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