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Cited by 309Nanoparticles: synthesis and applicationsMine drainage and remediation techniquesSelenium in Biological Systems

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Aerobic biogenesis of selenium nanospheres by Bacillus cereus isolated from coalmine soil

Open access
Dhanjal, Soniya, Cameotra, Swaranjit·2010·Microbial Cell Factories
DOI 10.1186/1475-2859-9-52PMC PMC2909957
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Abstract

BACKGROUND: Microorganisms that are exposed to pollutants in the environment, such as metals/metalloids, have a remarkable ability to fight the metal stress by various mechanisms. These metal-microbe interactions have already found an important role in biotechnological applications. It is only recently that microorganisms have been explored as potential biofactories for synthesis of metal/metalloid nanoparticles. Biosynthesis of selenium (Se 0) nanospheres in aerobic conditions by a bacterial strain isolated from the coalmine soil is reported in the present study. RESULTS: The strain CM100B, identified as Bacillus cereus by morphological, biochemical and 16S rRNA gene sequencing [GenBank:GU551935.1] was studied for its ability to generate selenium nanoparticles (SNs) by transformation of toxic selenite (SeO3(2-)) anions into red elemental selenium (Se 0) under aerobic conditions. Also, the ability of the strain to tolerate high levels of toxic selenite ions was studied by challenging the microbe with different concentrations of sodium selenite (0.5 mM-10 mM). ESEM, AFM and SEM studies revealed the spherical Se 0 nanospheres adhering to bacterial biomass as well as present as free particles. The TEM microscopy showed the accumulation of spherical nanostructures as intracellular and extracellular deposits. The deposits were identified as element selenium by EDX analysis. This is also indicated by the red coloration of the culture broth that starts within 2-3 h of exposure to selenite oxyions. Selenium nanoparticles (SNs) were further characterized by UV-Visible spectroscopy, TEM and zeta potential measurement. The size of nanospheres was in the range of 150-200 nm with high negative charge of -46.86 mV. CONCLUSIONS: This bacterial isolate has the potential to be used as a bionanofactory for the synthesis of stable, nearly monodisperse Se 0 nanoparticles as well as for detoxification of the toxic selenite anions in the environment. A hypothetical mechanism for the biogenesis of selenium nanoparticles (SNs) involving membrane associated reductase enzyme(s) that reduces selenite (SeO3(2-)) to Se 0 through electron shuttle enzymatic metal reduction process has been proposed.

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  • Soil bacteria can form (nano)-sized Se(0) from selenate and selenite.

    “A number of studies have reported that soil bacteria can form (nano)-sized Se(0) from selenate and selenite [125,126,127,128,129]”

    From Selenium Cycling Across Soil-Plant-Atmosphere Interfaces: A Critical Review (2015) · cites this paper as [127]

    The Results and Discussion section on localization of reduced selenite reports that a soil-derived Bacillus cereus isolate produced 150–200 nm selenium nanospheres from selenite. The paper discusses bacterial reduction of selenate to elemental selenium, but our copy does not show that this isolate produced nanosized Se(0) from selenate. (LLM, conf=0.85)

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Research works printed but not linked: 33

  1. Zhang Y Zhang J Wang HY Chen HY Synthesis of selenium nanoparticles in the presence of polysaccharides Mater Lett 2004 58 2590 2594 10.1016/j.matlet.2004.03.031 · DOI
  2. Ip C Selenium and ER stress response: Implication and exploitation for cancer therapy Abstr Proc Int 2006 63 Conference on selenium in Biology and Medicine, Univ. Wisconsin, Madison
  3. Gao X Zhang J Zhang L Hollow sphere selenium nanoparticles. Their in-vitro anti-hydroxyl radical effect Adv Mater 2002 14 290 293 10.1002/1521-4095(20020219)14:4<290::AID-ADMA290>3.0.CO;2-U · DOI
  4. Wang H Zhang J Yu H Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: Comparison with selenomethionine in mice Free Radic Biol Med 2007 42 1524 1533 10.1016/j.freeradbiomed.2007.02.013 17448899 · DOI
  5. Peng D Zhang J Liu Q Taylor EW Size effect of elemental selenium nanoparticles (Nano-Se) at supranutritional levels on selenium accumulation and glutathione S-transferase activity J Inorg Biochem 2007 101 1457 63 10.1016/j.jinorgbio.2007.06.021 17664013 · DOI
  6. Shin Y Blackwood JM Bae I-T Arey BW Exarhos GJ Synthesis and stabilization of selenium nanoparticles on cellulose nanocrystal Mater Lett 2007 61 4297 4300 10.1016/j.matlet.2007.01.091 · DOI
  7. Thakkar KN Mhatre SS Parikh RY Biological synthesis of metallic nanoparticles Nanomedicine: NBM 2009 6 257 262 19616126
  8. Narasingarao P Haggblom MM Identification of anaerobic selenate-respiring bacteria from aquatic sediments Appl Environ Microbiol 2007 73 3519 3527 10.1128/AEM.02737-06 17435005 PMC1932684 · DOI
  9. Lortie L Gould WD Rajan S Meeready RGL Cheng KJ Reduction of elemental selenium by a Pseudomonas stutzeri isolate Appl Environ Microbiol 1992 58 4042 4044 16348829 PMC183223
  10. Oremland RS Blum JS Culbertson CW Visscher PT Miller LG Dowdle P Strohmaier FE Isolation, Growth and Metabolism of an Obligately Anaerobic, Selenate-Respiring Bacterium, Strain SES-3 Appl Environ Microbiol 1994 60 3011 3019 16349362 PMC201757
  11. Sabaty M Avazeri C Pignol D Vermeglio A Characterization of the reduction of selenate and tellurite by nitrate reductases Appl Environ Microbiol 2001 67 5122 5126 10.1128/AEM.67.11.5122-5126.2001 11679335 PMC93280 · DOI
  12. Oremland RS Herbel MJ Blum JS Langley S Beveridge TJ Ajayan PM Sutto T Ellis AV Curran S Structural and spectral features of selenium nanospheres produced by Se-respiring bacteria Appl Environ Microbiol 2004 70 52 60 10.1128/AEM.70.1.52-60.2004 14711625 PMC321302 · DOI
  13. Tejo Prakash N Sharma N Prakash R Raina KK Fellowes J Pearce CI Lloyd JR Pattrick RAD Aerobic microbial manufacture of nanoscale selenium: exploiting nature's bio-nanomineralization potential Biotechnol Lett 2009 31 1857 1862 10.1007/s10529-009-0096-0 19690806 · DOI
  14. Yadav V Sharma N Prakash R Raina KK Bharadwaj LM Tejo Prakash N Generation of Selenium containing Nano-structures by soil bacterium, Pseudomonas aeruginosa Biotechnol 2008 7 299 304 10.3923/biotech.2008.299.304 · DOI
  15. Tamura K Dudley J Nei M Kumar S Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 Mol Biol Evol 2007 24 1596 1599 10.1093/molbev/msm092 17488738 · DOI
  16. Yudovich Ya E Ketris MP Selenium in coal: A review Int J Coal Geol 2006 67 112 126 10.1016/j.coal.2005.09.003 · DOI
  17. Dungan RS Yates SR Frankenberger WT Transformations of selenate and selenite by Stenotrophomonas maltophilia isolated from a seleniferous agricultural drainage pond sediment Environ Microbiol 2003 5 287 295 10.1046/j.1462-2920.2003.00410.x 12662176 · DOI
  18. Pages D Rose J Conrod S Cuine S Carrier P Heulin T Achouak W Heavy Metal Tolerance in Stenotrophomonas maltophilia Plos One 2008 e1539 doi: 10.1371/journal. pone. 0001539 10.1371/journal.pone.0001539 18253487 PMC2212715 · DOI
  19. Kessi J Ramuz M Wehrli E Spycher M Bachofen R Reduction of selenite and detoxifixation of elemental selenium by the phototrophic bacterium Rhodospirillum rubrum Appl Environ Microbiol 1999 65 4734 4740 10543779 PMC91637
  20. Roux M Sarret G Paintrand IP Fontecave M Coves J Mobilization of selenite by Ralstonia metallidurans CH34 Appl Environ Microbiol 2001 67 769 773 10.1128/AEM.67.2.769-773.2001 11157242 PMC92646 · DOI
  21. Lin ZH Wang CRC Evidence on the size-dependent absorption spectral evolution of selenium nanoparticles Mater Chem Phy 2005 92 591 594 10.1016/j.matchemphys.2005.02.023 · DOI
  22. Losi M Frankenberger WT Jr Reduction of selenium by Enterobacter cloacae SLD1a-1: isolation and growth of bacteria and its expulsion of selenium particles Appl Environ Microbiol 1997 63 3079 3084 16535668 PMC1389223
  23. Switzer-Blum J Bindi AB Buzzelli J Stolz JF Oremland RS Bacillus arsenicoselenatis sp. nov. and Bacillus selenitireducens sp. nov.: two haloalkaliphiles from Mono Lake California that respire oxyions of selenium and arsenic Arch Microbiol 1998 171 19 30 10.1007/s002030050673 9871015 · DOI · Paper page
  24. Chasteen TG Confusion between dimethyl selenenyl sulfide and dimethyl selenone released by bacteria Appl Organomet Chem 1993 7 335 342 10.1002/aoc.590070507 · DOI
  25. Swearingen JW Jr Fuentes DE Araya MA Plishker MF Saavedra CP Chasteen TG Va´squez CC Expression of the ubiE Gene of Geobacillus stearothermophilus V in Escherichia coli K-12 Mediates the Evolution of Selenium Compounds into the Headspace of Selenite- and Selenate-Amended Cultures Appl Environ Microbiol 2006 72 963 967 10.1128/AEM.72.1.963-967.2006 16391146 PMC1352272 · DOI
  26. Zehr JP Oremland RS Reduction of selenate to selenide by sulfate respiring bacteria: experiments with cell suspensions and estuarine sediments Appl Environ Microbiol 1987 53 1365 1369 16347366 PMC203871
  27. Ranjard L Prigent-Combaret C Nazaret S Cournoyer B Methylation of Inorganic and Organic Selenium by the Bacterial Thiopurine Methyltransferase J Bacteriol 2002 184 3146 3149 10.1128/JB.184.11.3146-3149.2002 12003960 PMC135077 · DOI
  28. Yurkov VV Beatty JT Aerobic anoxygenic phototrophic bacteria Microbiol Mol Biol Rev 1998 62 695 724 9729607 PMC98932
  29. Yanke LJ Bryant RD Laishley EJ Hydrogenase (I) of Clostridrium pasteurianum functions a novel selenite reductase Anaerobe 1995 1 61 67 10.1016/S1075-9964(95)80457-9 16887509 · DOI
  30. Etezad SM Khajeh K Soudi M Ghazvini PTM Dabirmanesh B Evidence on the presence of two distinct enzymes responsible for the reduction of selenate and tellurite in Bacillus sp. STG-83 Enzyme Microb Tech 2009 45 1 6 10.1016/j.enzmictec.2009.04.004 · DOI
  31. Hunter WJ Manter DK Reduction of selenite to elemental red selenium by Pseudomonas sp. strain CA5 Curr Microbiol 2009 58 493 498 10.1007/s00284-009-9358-2 19189180 · DOI
  32. Belzile N Wu GJ Chen YW Appanna VD Detoxification of selenite and mercury by reduction and mutual protection in the assimilation of both elements by Pseudomonas fluorescens Sci Total Environ 2006 367 704 714 10.1016/j.scitotenv.2006.03.008 16626785 · DOI
  33. Yamada A Miyashita M Inoue K Matsunaga T Extracellular reduction of selenite by a novel marine photosynthetic bacterium Appl Microbiol Biotechnol 1997 48 367 372 10.1007/s002530051064 9352678 · DOI

Cited by

Sorted by citations

  • Selenium Cycling Across Soil-Plant-Atmosphere Interfaces: A Critical Review

    Winkel, L., Vriens, B., Jones, G., Schneider, L., Pilon-Smits, E., & Bañuelos, G. (2015) · Nutrients · doi:10.3390/nu7064199 · Cited by 476

    “A number of studies have reported that soil bacteria can form (nano)-sized Se(0) from selenate and selenite [125–129] but it is unclear if microbial production of (nano) Se(0) is important in natural environments and what the fate of biogenic nanoparticular Se is in soil systems.”
    Background

Metadata

Authors
Dhanjal, S., & Cameotra, S.
Affiliations
Institute of Microbial Technology (IN)
Corresponding author
Cameotra, Swaranjit
Venue
Microbial Cell Factories
Publisher
Springer Science and Business Media LLC
Published
2010
Volume
9
Issue
1
Pages
52
ISSN
1475-2859 (ISSN-L)
DOI
10.1186/1475-2859-9-52
PMC
PMC2909957
OpenAlex
W2131814640
Cited by
309
References
1
Citations by year
2026: 11 · 2025: 12 · 2024: 20 · 2023: 25 · 2022: 25 · 2021: 32 · 2020: 24 · 2019: 21 · 2018: 27 · 2017: 20 · 2016: 30 · 2015: 24 · 2014: 18 · 2013: 6 · 2012: 9
FWCI
3.14
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Microbial Cell Factories (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Council of Scientific and Industrial Research, India
  • CSIR - Institute of Microbial Technology
Topics
  • Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
  • Physical Sciences › Environmental Science › Environmental Chemistry › Mine drainage and remediation techniques
  • Health Sciences › Nursing › Nutrition and Dietetics › Selenium in Biological Systems
Keywords
Bacillus cereus, selenite reduction, selenium nanoparticles, metal tolerance
MeSH terms
Bacillus cereus, Coal Mining, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S, Soil Microbiology, Sodium Selenite, Biomass, Nanospheres

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