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Effects of iron on the growth, biofilm formation and virulence of Klebsiella pneumoniae causing liver abscess
Open accessPublished version of a preprint
Abstract
Background: Klebsiella pneumoniae is considered the most clinically relevant species of Enterobacteriaceae, known to cause severe infections including liver abscesses. To the best of our knowledge, a large proportion of iron in the human body is accumulated and stored in the liver. We hypothesize that increased iron availability is an important factor driving liver abscess formation and we therefore aim to understand the effects of iron on K. pneumoniae causing liver abscesses. Results: All tested K. pneumoniae clinical isolates, including those isolated from liver abscesses and other abdominal invasive infection sites, grew optimally when cultured in LB broth supplemented with 50 μM iron and exhibited the strongest biofilm formation ability under those conditions. Decreased growth and biofilm formation ability were observed in all tested strains when cultured with an iron chelator ( P < 0.05). The infection model of G. mellonella larvae indicated the virulence of liver abscess-causing K. pneumoniae (2/3) cultured in LB broth with additional iron was significantly higher than those under iron-restricted conditions ( P < 0.05). The relative expression levels of the four siderophore genes ( iucB , iroB , irp1 , entB ) in K. pneumoniae strains isolated from liver abscesses cultured with additional iron were lower than those under iron-restricted conditions ( P < 0.05). Conclusions: It is suggested by our research that iron in the environment can promote growth, biofilm formation and enhance virulence of K. pneumoniae causing liver abscesses . A lower expression of siderophore genes correlates with increased virulence of liver abscess-causing K. pneumoniae . Further deeper evaluation of these phenomena is warranted.
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Metadata
- Authors
- Chen, T., Dong, G., Zhang, S., Zhang, X., Zhao, Y., Cao, J., Zhou, T., & Wu, Q.
- Affiliations
- First Affiliated Hospital of Wenzhou Medical University (CN)
- Hubei University of Medicine (CN)
- Wenzhou Medical University (CN)
- Corresponding author
- Zhou, Tieli
- Wu, Qing
- Venue
- BMC Microbiology
- Published
- 2020-02-18
- Volume
- 20
- Issue
- 1
- Pages
- 36-36
- ISSN
- 1471-2180 (ISSN-L)
- PMC
- PMC7027070
- OpenAlex
- W3009771761
- Cited by
- 78
- References
- 2
- Citations by year
- 2026: 15 · 2025: 18 · 2024: 12 · 2023: 12 · 2022: 10 · 2021: 6 · 2020: 5
- FWCI
- 2.66
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- BMC Microbiology (publishedVersion, cc-by)
- Research Square (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (No.81971986)
- Health Department of Zhejiang Province of the People's Republic of China (No.2019KY098)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Immunology and Microbiology › Microbiology › Enterobacteriaceae and Cronobacter Research
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Keywords
- Klebsiella pneumoniae, liver abscess, iron availability, biofilm formation, bacterial virulence, siderophores, iron chelation, Galleria mellonella infection model, bacterial growth
- MeSH terms
- Animals, Culture Media, Disease Models, Animal, Humans, Iron, Iron Chelating Agents, Klebsiella pneumoniae, Lepidoptera, Liver Abscess, Virulence, Gene Expression Regulation, Bacterial, Biofilms, Virulence Factors
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