The DOI matches its Crossref record; 90 of the 90 references it cites were found in a registry or an index.
Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering
Open accessAbstract
Traditionally, generation of new plants with improved or desirable features has relied on laborious and time-consuming breeding techniques. Genome-editing technologies have led to a new era of genome engineering, enabling an effective, precise, and rapid engineering of the plant genomes. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) has emerged as a new genome-editing tool, extensively applied in various organisms, including plants. The use of CRISPR/Cas9 allows generating transgene-free genome-edited plants (“null segregants”) in a short period of time. In this review, we provide a critical overview of the recent advances in CRISPR/Cas9 derived technologies for inducing mutations at target sites in the genome and controlling the expression of target genes. We highlight the major breakthroughs in applying CRISPR/Cas9 to plant engineering, and challenges toward the production of null segregants. We also provide an update on the efforts of engineering Cas9 proteins, newly discovered Cas9 variants, and novel CRISPR/Cas systems for use in plants. The application of CRISPR/Cas9 and related technologies in plant engineering will not only facilitate molecular breeding of crop plants but also accelerate progress in basic research.
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Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
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Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437
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Search-and-replace genome editing without double-strand breaks or donor DNA
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Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
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In vivo genome editing using Staphylococcus aureus Cas9
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Cited by
Gene editing for plant resistance to abiotic factors: a systematic review
Nascimento, F. D. S., Rocha, A. D. J., Soares, J. M. D. S., Mascarenhas, M. S., Ferreira, M. D. S., Morais Lino, L. S., Ramos, A. P. D. S., Diniz, L. E. C., Mendes, T. A. D. O., Ferreira, C. F., et al. (2023) · Plants · doi:10.3390/plants12020305 · Cited by 71
Revisiting CRISPR/Cas-mediated crop improvement: special focus on nutrition
Kaul, T., Sony, S. K., Verma, R., Motelb, K. F. A., Prakash, A. T., Eswaran, M., Bharti, J., Nehra, M., & Kaul, R. (2020) · Journal of Biosciences · doi:10.1007/s12038-020-00094-7 · Cited by 33
Karunarathne, S., Walker, E., Sharma, D., Li, C., & Han, Y. (2023) · Journal of Zhejiang University-SCIENCE B · doi:10.1631/jzus.b2200552 · Cited by 18
“The working mechanism of CRISPR is well explained in many recent reviews (Najera et al., 2019; McCarty et al., 2020; Wada et al., 2020; Nejat et al., 2022).”
Metadata
- Authors
- Wada, N., Ueta, R., Osakabe, Y., & Osakabe, K.
- Venue
- BMC Plant Biology
- Published
- 2020-05-25
- Volume
- 20
- Issue
- 1
- Pages
- 234-234
- ISSN
- 1471-2229 (ISSN-L)
- OpenAlex
- W3031743663
- Cited by
- 266
- References
- 90
- FWCI
- 9.75
- Type
- journal article
- Language
- en
- Open access
- gold
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- Wada, N., Ueta, R., Osakabe, Y., & Osakabe, K. (2020). Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering. BMC Plant Biology. https://doi.org/10.1186/s12870-020-02385-5
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