Changes in microbiota during experimental human Rhinovirus infection
Open accessAbstract
BACKGROUND: Human Rhinovirus (HRV) is responsible for the majority of common colds and is frequently accompanied by secondary bacterial infections through poorly understood mechanisms. We investigated the effects of experimental human HRV serotype 16 infection on the upper respiratory tract microbiota. METHODS: Six healthy volunteers were infected with HRV16. We performed 16S ribosomal RNA-targeted pyrosequencing on throat swabs taken prior, during and after infection. We compared overall community diversity, phylogenetic structure of the ecosystem and relative abundances of the different bacteria between time points. RESULTS: During acute infection strong trends towards increases in the relative abundances of Haemophilus parainfluenzae and Neisseria subflava were observed, as well as a weaker trend towards increases of Staphylococcus aureus. No major differences were observed between day-1 and day 60, whereas differences between subjects were very high. CONCLUSIONS: HRV16 infection is associated with the increase of three genera known to be associated with secondary infections following HRV infections. The observed changes of upper respiratory tract microbiota could help explain why HRV infection predisposes to bacterial otitis media, sinusitis and pneumonia.
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By 60 days, abundance of these bacteria had returned to baseline.
“By 60 days, abundance of these bacteria had returned to baseline.”
From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [119]
In the oropharyngeal compartment, rhinovirus infection was associated with a strong trend toward transient increases in the relative abundances of H. parainfluenzae, Neisseria subflava and a weak trend toward an increase in S. aureus.
“In the oropharyngeal compartment, rhinovirus infection was associated with a strong trend toward transient increases in the relative abundances of H. parainfluenzae, Neisseria subflava and a weak trend toward an increase in S. aureus (119).”
From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [119]
Experimental inoculation of hRV into the URT (nares) resulted in no significant changes in total read counts or of the main phyla (e.g., Actinobacteria, Firmicutes, and Proteobacteria) over time in nasopharyngeal samples or throat swabs.
“These studies reported no significant changes in total read counts or of the main phyla (e.g., Actinobacteria, Firmicutes, and Proteobacteria) over time in nasopharyngeal samples (73) or throat swabs (119).”
From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [119]
The number of subjects were small in both studies, limiting the power to detect changes over time.
“However, the number of subjects were small in both studies, limiting the power to detect changes over time.”
From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [119]
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References 43 · Cited by 50
- Authors
- Hofstra, J. J., Matamoros, S., van de Pol, M. A., de Wever, B., Tanck, M. W., Wendt-Knol, H., Deijs, M., van der Hoek, L., Wolthers, K. C., Molenkamp, R., Visser, C. E., Sterk, P. J., Lutter, R., & de Jong, M. D.
- Affiliations
- Amsterdam UMC Location University of Amsterdam (NL)
- Corresponding author
- Hofstra, J. J.
- Venue
- BMC Infectious Diseases
- Published
- 2015-08-14
- Volume
- 15
- Issue
- 1
- Pages
- 336-336
- ISSN
- 1471-2334 (ISSN-L)
- PMC
- PMC4659412
- OpenAlex
- W2124904833
- Cited by
- 50
- References
- 43
- Citations by year
- 2025: 4 · 2023: 5 · 2022: 5 · 2021: 4 · 2020: 7 · 2019: 4 · 2018: 9 · 2017: 6 · 2016: 6
- FWCI
- 5.49
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- BMC Infectious Diseases (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Pure Amsterdam UMC (acceptedVersion, other-oa)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Funders
- European Commission (602525, 115010)
- Innovative Medicines Initiative (115010)
- Topics
- Health Sciences › Medicine › Epidemiology › Respiratory viral infections research
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- human rhinovirus 16, upper respiratory tract microbiota, common cold, secondary bacterial infection, Haemophilus parainfluenzae, Neisseria subflava, Staphylococcus aureus, bacterial co-infection, bacterial otitis media, sinusitis, pneumonia
- MeSH terms
- Adolescent, Adult, Female, Humans, Male, Middle Aged, Neisseria, Pharynx, Picornaviridae Infections, Respiratory Tract Infections, Rhinovirus, RNA, Ribosomal, 16S, Staphylococcus aureus, Haemophilus parainfluenzae, Young Adult, Microbiota
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