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Cited by 52Bacteriophages and microbial interactionsEscherichia coli research studiesBacterial Genetics and Biotechnology

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Phage tailspike modularity and horizontal gene transfer reveals specificity towards E. coli O-antigen serogroups

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Pas, Célia, Latka, Agnieszka, Fieseler, Lars, Briers, Yves·2023·Virology Journal
DOI 10.1186/s12985-023-02138-4PMC PMC10408124

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Abstract

Background: The interaction between bacteriophages and their hosts is intricate and highly specific. Receptor-binding proteins (RBPs) of phages such as tail fibers and tailspikes initiate the infection process. These RBPs bind to diverse outer membrane structures, including the O-antigen, which is a serogroup-specific sugar-based component of the outer lipopolysaccharide layer of Gram-negative bacteria. Among the most virulent Escherichia coli strains is the Shiga toxin-producing E. coli (STEC) pathotype dominated by a subset of O-antigen serogroups. Methods: Extensive phylogenetic and structural analyses were used to identify and validate specificity correlations between phage RBP subtypes and STEC O-antigen serogroups, relying on the principle of horizontal gene transfer as main driver for RBP evolution. Results: We identified O-antigen specific RBP subtypes for seven out of nine most prevalent STEC serogroups (O26, O45, O103, O104, O111, O145 and O157) and seven additional E. coli serogroups (O2, O8, O16, O18, 4s/O22, O77 and O78). Eight phage genera ( Gamaleya-, Justusliebig-, Kaguna-, Kayfuna-, Kutter-, Lederberg-, Nouzilly- and Uetakeviruses ) emerged for their high proportion of serogroup-specific RBPs. Additionally, we reveal sequence motifs in the RBP region, potentially serving as recombination hotspots between lytic phages. Conclusion: The results contribute to a better understanding of mosaicism of phage RBPs, but also demonstrate a method to identify and validate new RBP subtypes for current and future emerging serogroups.

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Metadata

Authors
Pas, C., Latka, A., Fieseler, L., & Briers, Y.
Affiliations
  • Ghent University (BE)
  • ZHAW Zurich University of Applied Sciences (CH)
Corresponding author
Briers, Yves
ORCID
Venue
Virology Journal
Publisher
Springer Science and Business Media LLC
Published
2023-08-07
Volume
20
Issue
1
Pages
174-174
ISSN
1743-422X (ISSN-L)
DOI
10.1186/s12985-023-02138-4
PMC
PMC10408124
OpenAlex
W4385636902
Cited by
52
References
1
Citations by year
2026: 22 · 2025: 13 · 2024: 15 · 2023: 2
FWCI
8.41
Type
journal article
Language
en
Open access
Licence
Locations
  • Virology Journal (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Fonds Wetenschappelijk Onderzoek (1S79422N, 1240021N)
Topics
  • Life Sciences › Immunology and Microbiology › Virology › Bacteriophages and microbial interactions
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
receptor binding protein, horizontal gene transfer, Shiga toxin-producing Escherichia coli, phage-host specificity, phage tail fiber, recombination hotspots, phage evolution, lytic phages
MeSH terms
Escherichia coli Infections, Feces, Humans, Phylogeny, O Antigens, Gene Transfer, Horizontal, Shiga-Toxigenic Escherichia coli, Serogroup

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