Acceleration of bone union by in situ-formed hydrogel containing bone morphogenetic protein-2 in a mouse refractory fracture model
Open accessAbstract
Background: An enzymatic crosslinking strategy using hydrogen peroxide and horseradish peroxidase is receiving increasing attention for application with in situ-formed hydrogels (IFHs). Several studies have reported the application of IFHs in cell delivery and tissue engineering. IFHs may also be ideal carrier materials for bone repair, although their potential as a carrier for bone morphogenetic protein (BMP)-2 has yet to be examined. Here, we examined the effect of an IFH made of hyaluronic acid (IFH-HA) containing BMP-2 in promoting osteogenesis in a mouse refractory fracture model. Methods: Immediately following a fracture procedure, animals either received no treatment (control) or an injection of IFH-HA/PBS or IFH-HA containing 2 μg BMP-2 (IFH-HA/BMP-2) into the fracture site (n = 16, each treatment). Results: Fracture sites injected with IFH-HA/BMP-2 showed significantly greater bone volume, bone mineral content, and bone union compared with sites receiving no treatment or treated with IFH-HA/PBS alone (each n = 10). Gene expression levels of osteogenic markers, Alpl, Bglap, and Osx, were significantly raised in the IFH-HA/BMP-2 group compared to the IFH-HA/PBS and control groups (each n = 6). Conclusion: IFH-HA/BMP-2 may contribute to the treatment of refractory fractures.
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Natural polymer carriers may be advantageous if they contain cell binding motifs that promote osteoconductivity.
“Natural polymer carriers may be advantageous if they contain cell binding motifs that promote osteoconductivity. [9–12]”
From Keratin Scaffold Formulation Impacts rhBMP-2 Biodistribution and Bone Regeneration in a Rat Femur Defect Model (2025) · cites this paper as [11]
The Background (page unavailable) describes hydrogels made from natural polysaccharides as potentially useful carriers for bone repair. It does not say that their advantage depends on cell-binding motifs or that such motifs promote osteoconductivity. (LLM, conf=0.82)
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References 38 · Cited by 8
- Authors
- Shoji, S., Uchida, K., Satio, W., Sekiguchi, H., Inoue, G., Miyagi, M., Takata, K., Yokozeki, Y., & Takaso, M.
- Affiliations
- Kitasato University (JP)
- Shonan University of Medical Sciences (JP)
- Corresponding author
- Uchida, Kentaro
- Venue
- Journal of Orthopaedic Surgery and Research
- Published
- 2020-09-18
- Volume
- 15
- Issue
- 1
- Pages
- 426-426
- ISSN
- 1749-799X (ISSN-L)
- PMC
- PMC7501615
- OpenAlex
- W3087312319
- Cited by
- 8
- References
- 38
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 4 · 2022: 2
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Journal of Orthopaedic Surgery and Research (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- JSPS KAKENHI (17K16701)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Epidemiology › Bone fractures and treatments
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- BMP-2, hyaluronic acid, enzymatic crosslinking, hydrogen peroxide, horseradish peroxidase, refractory fractures, bone union, bone formation, bone regeneration, osteogenic gene expression
- MeSH terms
- Sp7 Transcription Factor, Alkaline Phosphatase, Animals, Disease Models, Animal, Femoral Fractures, Femur, Mice, Inbred C57BL, Organ Size, Osteogenesis, Bone Density, Injections, Intralesional, Gene Expression, Hydrogels, Bone Morphogenetic Protein 2
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