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Cited by 29Toxoplasma gondii Research StudiesMicroRNA in disease regulationMicrobial infections and disease research

Expression profile of microRNAs in porcine alveolar macrophages after Toxoplasma gondii infection

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Li, Senyang, Yang, Jing, Wang, Luyao, Du, Fen, Zhao, Junlong, Fang, Rui·2019·Parasites & Vectors
DOI 10.1186/s13071-019-3297-yPMC PMC6350279
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Abstract

BACKGROUND: Toxoplasma gondii is an apicomplexan protozoan parasite that can cause serious clinical illnesses in both humans and animals. microRNAs (miRNAs) are non-protein-coding RNAs that can regulate the expression of target genes. A previous study found that many miRNAs were differentially expressed after T. gondii infection and exert significant effects and revealed that both host survival and the virulence of different strains can be regulated by different miRNAs. Macrophages play an important role in T. gondii infection, but few studies have investigated the relationship between miRNAs and porcine alveolar macrophages infected with T. gondii. METHODS: Porcine alveolar macrophages (3D4-21) were infected with the RH (Type I) and Me49 (Type II) strains of T. gondii for 12 h and 24 h and then harvested. miRNA libraries were generated using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA), and the miRNA expression levels were estimated based on transcripts per million reads (TPM). RESULTS: Our study generated six miRNA expression profiles from macrophages infected with RH and Me49 compared with the control groups. The comparison of the T. gondii-infected and uninfected samples identified 81 differentially expressed miRNAs, including 36 novel miRNAs and 45 mature miRNAs. The target genes of these differentially expressed miRNAs were predicted using miRanda software, and ssc-miR-127 and ssc-miR-143-3p were predicted to regulate nitric oxide synthase 1 (NOS1) and nitric oxide synthase 3 (NOS3), respectively, which play essential roles in synthesizing nitric oxide (NO) by oxidizing L-arginine. These genes were differentially expressed in both the RH- and Me49-infected groups. A KEGG enrichment analysis indicated that the predicted target genes were involved in multiple signaling pathways, including FcγR-mediated phagocytosis, the AMPK signaling pathway, the mTOR signaling pathway, and the FcγRI signaling pathway, all of which are indispensable for the normal functioning of porcine alveolar macrophages. CONCLUSIONS: Our results provide data on the miRNA profile of porcine alveolar macrophages infected with T. gondii. To our knowledge, this study provides the first demonstration of the relationship between miRNA and macrophages of swine origin. Understanding the functions of these regulated miRNAs will aid the investigation of T. gondii infectious diseases, and the differentially expressed miRNAs might be candidate drug targets for T. gondii infection in pigs.

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  • SYK kinase is crucial for FcγR-mediated phagocytosis and necessary for PI3-kinase activation and regulation of Rac2 and Vav3 activity.

    “SYK kinase is crucial for FcγR-mediated phagocytosis and is necessary for PI3-kinase activation, and further regulation of Rac2 and Vav3 activity.”

    From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [214]

    The Discussion says SYK kinases are essential for FcγR-mediated phagocytosis and crucial for downstream PI3-kinase activation. It also says Rac2 and Vav3 play notable roles in the pathway, but does not state that SYK regulates their activity. (LLM, conf=0.86)

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Cited by

Sorted by citations

Authors
Li, S., Yang, J., Wang, L., Du, F., Zhao, J., & Fang, R.
Affiliations
  • Huazhong Agricultural University (CN)
  • Hubei Provincial Center for Disease Control and Prevention (CN)
Corresponding author
  • Zhao, Junlong
  • Fang, Rui
ORCID
Venue
Parasites & Vectors
Publisher
Springer Science and Business Media LLC
Published
2019-01-29
Volume
12
Issue
1
Pages
65-65
ISSN
1756-3305 (ISSN-L)
DOI
10.1186/s13071-019-3297-y
PMC
PMC6350279
OpenAlex
W2947237849
Cited by
29
References
0
Citations by year
2026: 2 · 2025: 3 · 2024: 6 · 2023: 3 · 2022: 5 · 2021: 5 · 2020: 3 · 2019: 2
FWCI
2.89
Type
journal article
Language
en
Open access
Licence
Locations
  • Parasites & Vectors (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (31572510)
  • Natural Science Foundation of Hubei Province (2017CFA020)
  • Da Bei Nong Group Promoted Project for Young Scholar of HZAU (2017DBN001)
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Toxoplasma gondii Research Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords
Toxoplasma gondii, porcine alveolar macrophages, microRNA expression profiles, differentially expressed miRNAs, novel miRNAs, nitric oxide synthase 1, nitric oxide synthase 3, nitric oxide, AMPK signaling, mTOR signaling, host-parasite interactions, swine toxoplasmosis, miRNA target genes, KEGG pathway enrichment
MeSH terms
Animals, Cells, Cultured, Humans, Swine, Swine Diseases, Toxoplasmosis, Animal, Macrophages, Alveolar, MicroRNAs, Transcriptome, Real-Time Polymerase Chain Reaction, Gene Ontology

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