The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Widespread intron retention diversifies most cancer transcriptomes
Open accessAbstract
Background: Somatic mutations affecting components of the RNA splicing machinery occur with high frequencies across many tumor types. These mutations give rise to distinct alterations in normal splice site and exon recognition, such as unusual 3′ splice site preferences, that likely contribute to tumorigenesis. Methods: We analyzed genome-wide patterns of RNA splicing across 805 matched tumor and normal control samples from 16 distinct cancer types to identify signals of abnormal cancer-associated splicing. Results: We found that abnormal RNA splicing, typified by widespread intron retention, is common across cancers even in the absence of mutations directly affecting the RNA splicing machinery. Almost all liquid and solid cancer types exhibited frequent retention of both alternative and constitutive introns relative to control normal tissues. The sole exception was breast cancer, where intron retention typified adjacent normal rather than cancer tissue. Different introns were preferentially retained in specific cancer types, although a small subset of introns enriched for genes encoding RNA splicing and export factors exhibited frequent retention across diverse cancers. The extent of intron retention correlated with the presence of IDH1 and IDH2 mutations in acute myeloid leukemia and across molecular subtypes in breast cancer. Many introns that were preferentially retained in primary cancers were present at high levels in the cytoplasmic mRNA pools of cancer cell lines. Conclusions: Our data indicate that abnormal RNA splicing is a common characteristic of cancers even in the absence of mutational insults to the splicing machinery, and suggest that intron-containing mRNAs contribute to the transcriptional diversity of many cancers.
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Cites
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“‘Spliceosomal mutations’ are enriched in diverse diseases, including myelodysplastic syndromes, lymphoid leukemias, and solid tumors of the lung, breast, pancreas, and eye, and most commonly cause specific missense changes to the SF3B1, SRSF2, and U2AF1 proteins [1–10].”
BackgroundFrequent pathway mutations of splicing machinery in myelodysplasia
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Cited by
RNA splicing dysregulation and the hallmarks of cancer
Bradley, R. K., & Anczuków, O. (2023) · Nature Reviews Cancer · doi:10.1038/s41568-022-00541-7 · Cited by 579
Self-citationTherapeutic targeting of splicing in cancer
Lee, S. C.-W., & Abdel-Wahab, O. (2016) · Nature Medicine · doi:10.1038/nm.4165 · Cited by 561
Intron retention is a source of neoepitopes in cancer
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Metadata
- Authors
- Dvinge, H., & Bradley, R. K.
- Venue
- Genome Medicine
- Published
- 2015-05-15
- Volume
- 7
- Issue
- 1
- Pages
- 45-45
- ISSN
- 1756-994X (ISSN-L)
- OpenAlex
- W2139199979
- Cited by
- 344
- References
- 16
- FWCI
- 10.42
- Type
- journal article
- Language
- en
- Open access
- gold
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