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Engineering of Nitrosomonas europaea to express Vitreoscilla hemoglobin enhances oxygen uptake and conversion of ammonia to nitrite
Open accessAbstract
Nitrosomonas europaea was transformed with a recombinant plasmid bearing the gene (vgb) encoding the hemoglobin (VHb) from the bacterium Vitreoscilla under control of the N. europaea amoC P1 promoter. Vgb was maintained stably and appeared to be expressed in the transformants at VHb levels of about 0.75 nmol/g wet weight. Expression of VHb in the N. europaea transformants was correlated with an approximately 2 fold increase in oxygen uptake rate by whole cells at oxygen concentrations in the range of 75-100% saturation, but no change in oxygen uptake rate at oxygen concentrations below 25% saturation. VHb expression was also correlated with an increase of as much as about 30% in conversion of ammonia to nitrite by growing cells. The results suggest that engineering of key aerobic wastewater bacteria to express bacterial hemoglobins may be a useful strategy to produce species with enhanced respiratory abilities.
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References 36 · Cited by 6
Research works printed but not linked: 35
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Metadata
- Authors
- Kunkel, S. A., Pagilla, K. R., & Stark, B. C.
- Affiliations
- Illinois Institute of Technology (US)
- Corresponding author
- Stark, Benjamin C
- Venue
- AMB Express
- Published
- 2015-08-01
- Volume
- 5
- Issue
- 1
- Pages
- 135-135
- ISSN
- 2191-0855 (ISSN-L)
- PMC
- PMC4522006
- OpenAlex
- W2128933863
- Cited by
- 6
- References
- 36
- Citations by year
- 2025: 1 · 2022: 1 · 2021: 1 · 2019: 1 · 2016: 2
- FWCI
- 0.42
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- AMB Express (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Oregon State University
- Topics
- Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Hemoglobin structure and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Nitrosomonas europaea, Vitreoscilla hemoglobin, ammonia oxidation, oxygen consumption, heterologous expression
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