Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression
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Abstract
Background: Candida tropicalis ( C. tropicalis ) is an important opportunistic pathogenic Candida species that can cause nosocomial infection. In this study, we analyzed the distribution and drug susceptibility of C. tropicalis and the relationship between ERG11 and UPC2 expression and resistance to azole antifungal agents. Methods: C. tropicalis was cultured and identified by Sabouraud Agar Medium, CHROM Agar Candida and ATB tests (Bio-Mérieux, France). Total RNA was extracted from the collected strains, and the ERG11 and UPC2 mRNA expression levels were analyzed by quantitative real-time PCR. Results: In total, 2872 clinical isolates of Candida , including 319 strains of C. tropicalis , were analyzed herein; they were mainly obtained from the Departments of Respiratory Medicine and ICU. The strains were predominantly isolated from airway secretion samples, and the detection trend in four years was mainly related to the type of department and specimens. The resistance rates of C. tropicalis to fluconazole, itraconazole and voriconazole had been increasing year by year. The mRNA expression levels of ERG11 and UPC2 in the fluconazole-resistant group were significantly higher than they were in the susceptible group. In addition, there was a significant positive linear correlation between these two genes in the fluconazole-resistant group. Conclusions: Overexpression of the ERG11 and UPC2 genes in C. tropicalis could increase resistance to azole antifungal drugs. The routine testing for ERG11 and UPC2 in high-risk patients in key departments would provide a theoretical basis for the rational application of azole antifungal drugs.
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UPC2 transcriptional level positively correlates with FLC resistance in Candida tropicalis.
“Wang et al. conducted an analysis of the transcriptional level of the UPC2 gene in 319 clinical strains of Candida tropicalis, revealing a linear positive correlation between the gene’s transcriptional level and FLC resistance in Candida tropicalis [16].”
From Disruption of the UPC2 Gene Enhances Fluconazole Antifungal Activity by Inhibiting HAC1 mRNA Splicing in Candida albicans (2026) · cites this paper as [16]
The Results section reports significantly higher UPC2 mRNA expression in fluconazole-resistant than susceptible C. tropicalis, supporting an association with resistance. But expression was measured in 50 strains selected from 319, not all 319; the reported linear positive correlation was between UPC2 and ERG11 expression within the resistant group, not between UPC2 expression and fluconazole resistance. (LLM, conf=0.92)
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References 34 · Cited by 52
- Authors
- Wang, D., An, N., Yang, Y., Yang, X., Fan, Y., & Feng, J.
- Affiliations
- First Affiliated Hospital of Chengdu Medical College (CN)
- Mianyang Central Hospital (CN)
- Corresponding author
- Feng, Jiafu
- Venue
- Antimicrobial Resistance & Infection Control
- Published
- 2021-03-15
- Volume
- 10
- Issue
- 1
- Pages
- 54-54
- ISSN
- 2047-2994 (ISSN-L)
- PMC
- PMC7958445
- OpenAlex
- W3111181992
- Cited by
- 52
- References
- 34
- Citations by year
- 2026: 10 · 2025: 11 · 2024: 12 · 2023: 11 · 2022: 7 · 2021: 1
- FWCI
- 2.45
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Antimicrobial Resistance and Infection Control (publishedVersion, cc-by)
- Research Square (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- the Chengdu Medical College program (CYZYB20-10)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
- Keywords
- Candida tropicalis, azole resistance, fluconazole resistance, ERG11, UPC2, gene expression, antifungal susceptibility, clinical isolates, opportunistic fungal infection, nosocomial infections, respiratory secretions, voriconazole, itraconazole
- MeSH terms
- Antifungal Agents, Azoles, China, Fungal Proteins, Humans, Fluconazole, Itraconazole, Drug Resistance, Fungal, Candida tropicalis, Voriconazole
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