The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.
Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue
Open accessAbstract
Clinical use of adipose-derived stem cells (ASCs) for a variety of indications is rapidly expanding in medicine. Most commonly, ASCs are isolated at the point of care from lipoaspirate tissue as the stromal vascular fraction (SVF). The cells are immediately administered to the patient as an injection or used to enrich fat grafts. Isolation of ASCs from adipose tissue is a relatively simple process performed routinely in cell biology laboratories, but isolation at the point of care for immediate clinical administration requires special methodology to prevent contamination, ensure integrity of clinical research and comply with regulatory requirements. A lack of practical laboratory experience, regulatory uncertainty and a relative paucity of objective published data can make selection of the optimum separation method for specific indications a difficult task for the clinician and can discourage clinical adoption. In this paper, we discuss the processes which can be used to separate SVF cells from fat tissue. We compare the various mechanical and enzymatic methods. We discuss the practical considerations involved in selecting an appropriate method from a clinical perspective. Studies consistently show that breakdown of the extracellular matrix achieved with proteolytic enzymes affords significantly greater efficiency to the separation process. SVF isolated through mechanical methods is equally safe, less costly and less time consuming but the product contains a higher frequency of blood mononuclear cells and fewer progenitor cells. Mechanical methods can provide a low cost, rapid and simple alternative to enzymatic isolation methods, and are attractive when smaller quantities of ASCs are sufficient.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“The most commonly used tissue dissociation method is enzymatic digestion, which disolves the ECM46.”
From Organoids (2022) · cites this paper as [46]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based Therapies
Zuk, P. A., Zhu, M., Mizuno, H., Huang, J., Futrell, J. W., Katz, A. J., Benhaim, P., Lorenz, H. P., & Hedrick, M. H. (2001) · Tissue Engineering · doi:10.1089/107632701300062859 · Cited by 7,956
Liao, J., Wei, Q., Zou, Y., Fan, J., Song, D., Cui, J., Zhang, W., Zhu, Y., Ma, C., Hu, X., et al. (2017) · Cellular Physiology and Biochemistry · doi:10.1159/000471945 · Cited by 2,742
Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells
Rehman, J., Traktuev, D., Li, J., Merfeld-Clauss, S., Temm-Grove, C. J., Bovenkerk, J. E., Pell, C. L., Johnstone, B. H., Considine, R. V., & March, K. L. (2004) · Circulation · doi:10.1161/01.cir.0000121425.42966.f1 · Cited by 2,269
Bourin, P., Bunnell, B. A., Casteilla, L., Dominici, M., Katz, A. J., March, K. L., Redl, H., Rubin, J. P., Yoshimura, K., & Gimble, J. M. (2013) · Cytotherapy · doi:10.1016/j.jcyt.2013.02.006 · Cited by 1,890 · cited 1×
“SVF contains a variety of cells including macrophages, various blood cells, pericytes, fibroblasts, smooth muscle cells, vascular endothelial progenitors and adipose-derived stem cells (Yoshimura et al. 2006; Bourin et al. 2013; Han et al. 2010; McIntosh et al. 2006; Bonab et al. 2006; Yoshimura et al. 2009).”
BackgroundPlanat-Benard, V., Silvestre, J.-S., Cousin, B., André, M., Nibbelink, M., Tamarat, R., Clergue, M., Manneville, C., Saillan-Barreau, C., Duriez, M., et al. (2004) · Circulation · doi:10.1161/01.cir.0000114522.38265.61 · Cited by 1,382
Mitchell, J. B., McIntosh, K. R., Zvonic, S., Garrett, S. M., Floyd, Z. E., Kloster, A. L., Halvorsen, Y. D. C., Storms, R. W., Goh, B. C. K., Kilroy, G. E., et al. (2005) · Stem Cells · doi:10.1634/stemcells.2005-0234 · Cited by 1,138
Aging of mesenchymal stem cell in vitro
Bonab, M. M., Alimoghaddam, K., Talebian, F., Ghaffari, S. H., Ghavamzadeh, A., & Nikbin, B. (2006) · BMC Cell Biology · doi:10.1186/1471-2121-7-14 · Cited by 914
Yoshimura, K., Shigeura, T., Matsumoto, D., Sato, T., Takaki, Y., Aiba‐Kojima, E., Sato, K., Inoue, K., Nagase, T., Koshima, I., et al. (2006) · Journal of Cellular Physiology · doi:10.1002/jcp.20636 · Cited by 803
Yield of human adipose-derived adult stem cells from liposuction aspirates
Aust, L., Devlin, B., Foster, S. J., Halvorsen, Y. D. C., Hicok, K., du Laney, T., Sen, A., Willingmyre, G. D., & Gimble, J. M. (2004) · Cytotherapy · doi:10.1080/14653240310004539 · Cited by 708
The Fate of Adipocytes after Nonvascularized Fat Grafting
D, H. E. M., D, H. K. M., D, H. S. M., D, N. A. M., Doi, K., Kuno, S., & Yoshimura, K. (2012) · Plastic & Reconstructive Surgery · doi:10.1097/prs.0b013e31824a2b19 · Cited by 664
Research works printed but not linked: 1
- Millan A, Landerholm T, Chapman JR (2014) Comparison between collagenase adipose digestion and Stromacell mechanical dissociation for mesenchymal stem cell separation. McNair Scholars J CSUS 15:86–101
Cited by
Zhao, Z., Chen, X., Dowbaj, A. M., Sljukic, A., Bratlie, K., Lin, L., Fong, E. L. S., Balachander, G. M., Chen, Z., Soragni, A., et al. (2022) · Nature Reviews Methods Primers · doi:10.1038/s43586-022-00174-y · Cited by 1,082
Bora, P., & Majumdar, A. S. (2017) · Stem Cell Research & Therapy · doi:10.1186/s13287-017-0598-y · Cited by 550
Intra-articular treatment options for knee osteoarthritis
Jones, I. A., Togashi, R., Wilson, M. L., Heckmann, N. D., & Vangsness, C. T. (2018) · Nature Reviews Rheumatology · doi:10.1038/s41584-018-0123-4 · Cited by 559
Gaur, M., Dobke, M. K., & Lunyak, V. V. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18010208 · Cited by 203
Adipose Tissue-Derived Stem Cells: Immunomodulatory Effects and Therapeutic Potential
Al-Ghadban, S. I., & Bunnell, B. A. (2020) · Physiology · doi:10.1152/physiol.00021.2019 · Cited by 190
Metadata
- Authors
- Aronowitz, J. A., Lockhart, R. A., & Hakakian, C. S.
- Affiliations
- University of Southern California (US)
- Corresponding author
- Aronowitz, Joel A.
- Venue
- SpringerPlus
- Published
- 2015-11-23
- Volume
- 4
- Issue
- 1
- Pages
- 713-713
- ISSN
- 2193-1801 (ISSN-L)
- PMC
- PMC4656256
- OpenAlex
- W2173002160
- Cited by
- 275
- References
- 55
- Citations by year
- 2026: 26 · 2025: 24 · 2024: 40 · 2023: 27 · 2022: 43 · 2021: 30 · 2020: 22 · 2019: 27 · 2018: 19 · 2017: 12 · 2016: 4
- FWCI
- 6.53
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- SpringerPlus (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Health Sciences › Dentistry › Periodontics › Periodontal Regeneration and Treatments
- Keywords
- adipose-derived stem cells, stromal vascular fraction, enzymatic cell isolation, proteolytic enzymes, lipoaspirate, progenitor cells, fat graft
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
