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Cited by 84Antibiotic Resistance in BacteriaAntibiotic Use and ResistanceInfections and bacterial resistance

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Carbapenem resistant Pseudomonas aeruginosa and Acinetobacter baumannii at Mulago Hospital in Kampala, Uganda (2007–2009)

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Kateete, David P., Nakanjako, Ritah, Namugenyi, Juliet, Erume, Joseph, Joloba, Moses L., Najjuka, Christine F.·2016·SpringerPlus
DOI 10.1186/s40064-016-2986-7PMC PMC4978656
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Abstract

BACKGROUND: Multidrug resistant Pseudomonas aeruginosa and Acinetobacter baumannii are common causes of health care associated infections worldwide. Carbapenems are effective against infections caused by multidrug resistant Gram-negative bacteria including Pseudomonas and Acinetobacter species. However, their use is threatened by the emergence of carbapenemase-producing strains. The aim of this study was to determine the prevalence of carbapenem-resistant P. aeruginosa and A. baumannii at Mulago Hospital in Kampala Uganda, and to establish whether the hospital environment harbors carbapenem-resistant Gram-negative rods. RESULTS: Between February 2007 and September 2009, a total of 869 clinical specimens were processed for culture and sensitivity testing yielding 42 (5 %) P. aeruginosa and 29 (3 %) A. baumannii isolates, of which 24 % (10/42) P. aeruginosa and 31 % (9/29) A. baumannii were carbapenem-resistant. Additionally, 80 samples from the hospital environment were randomly collected and similarly processed yielding 58 % (46/80) P. aeruginosa and 14 % (11/80) A. baumannii, of which 33 % (15/46) P. aeruginosa and 55 % (6/11) A. baumannii were carbapenem-resistant. The total number of isolates studied was 128. Carbapenemase genes detected were bla IMP-like (36 %, 9/25), bla VIM-like (32 %, 8/25), bla SPM-like (16 %, 4/25); bla NDM-1-like (4 %, 1/25) in carbapenem-resistant P. aeruginosa, and bla OXA-23-like (60 %, 9/15), bla OXA-24-like (7 %, 1/15), bla OXA-58-like (13 %, 2/15), and bla VIM-like (13 %, 2/15) in carbapenem-resistant A. baumannii. Furthermore, class 1 integrons were detected in 38 % (48/128) of P. aeruginosa and Acinetobacter, 37 % (26/71) of which were in clinical isolates and 39 % (22/57) in environment isolates. Gene cassettes were found in 25 % (12/48) of integron-positive isolates. These were aminoglycoside adenylyltransferase ant(4')-IIb (3 isolates); trimethoprim-resistant dihydrofolate reductase dfrA (2 isolates); adenyltransferase aadAB (3 isolates); QacE delta1 multidrug exporter (2 isolates); quinolone resistance pentapeptide repeat protein qnr (1 isolate); and metallo-β-lactamase genes bla VIM-4-like, bla IMP-19-like, and bla IMP-26-like (1 isolate each). Gene cassettes were missing in 75 % (36/48) of the integron-positive isolates. CONCLUSIONS: The prevalence of carbapenem-resistant P. aeruginosa and Acinetobacter among hospitalized patients at Mulago Hospital is low compared to rates from South-East Asia. However, it is high among isolates and in the environment, which is of concern given that the hospital environment is a potential source of infection for hospitalized patients and health care workers.

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Research works printed but not linked: 42

  1. Amudhan SM, Sekar U, Arunagiri K, Sekar B (2011) OXA beta-lactamase-mediated carbapenem resistance in Acinetobacter baumannii. Indian J Med Microbiol 29(3):269–274 · DOI
  2. Andreou LV (2013) Preparation of genomic DNA from bacteria. Methods Enzymol 529:143–151 · DOI
  3. Asthana S, Mathur P, Tak V (2014) Detection of carbapenemase production in Gram-negative bacteria. J Lab Physicians 6(2):69–75 · DOI
  4. Broberg CA, Palacios M, Miller VL (2013) Whole-genome draft sequences of three multidrug-resistant Klebsiella pneumoniae strains available from the American type culture collection. Genome Announc 1(3). doi: 10.1128/genomeA.00312-13 · DOI
  5. Carroll KC, Glanz BD, Borek AP, Burger C, Bhally HS, Henciak S, Flayhart D (2006) Evaluation of the BD Phoenix automated microbiology system for identification and antimicrobial susceptibility testing of Enterobacteriaceae. J Clin Microbiol 44(10):3506–3509 · DOI
  6. Falagas ME, Bliziotis IA, Kasiakou SK, Samonis G, Athanassopoulou P, Michalopoulos A (2005) Outcome of infections due to pandrug-resistant (PDR) Gram-negative bacteria. BMC Infect Dis 5:24 · DOI
  7. Fallah F, Borhan RS, Hashemi A (2013) Detection of bla(IMP) and bla(VIM) metallo-beta-lactamases genes among Pseudomonas aeruginosa strains. Int J Burns Trauma 3(2):122–124
  8. Fallah F, Noori M, Hashemi A, Goudarzi H, Karimi A, Erfanimanesh S, Alimehr S (2014) Prevalence of bla NDM, bla PER, bla VEB, bla IMP, and bla VIM genes among Acinetobacter baumannii isolated from two hospitals of Tehran, Iran. Scientifica 2014:245162 · DOI
  9. Fischer J, Rodriguez I, Schmoger S, Friese A, Roesler U, Helmuth R, Guerra B (2012) Escherichia coli producing VIM-1 carbapenemase isolated on a pig farm. J Antimicrob Chemother 67(7):1793–1795 · DOI
  10. Giamarellos-Bourboulis EJ, Sambatakou H, Galani I, Giamarellou H (2003) In vitro interaction of colistin and rifampin on multidrug-resistant Pseudomonas aeruginosa. J Chemother 15(3):235–238 · DOI
  11. Gootz TD, Marra A (2008) Acinetobacter baumannii: an emerging multidrug-resistant threat. Expert Rev Anti Infect Ther 6(3):309–325 · DOI
  12. Gu B, Tong M, Zhao W, Liu G, Ning M, Pan S (2007) Prevalence and characterization of class I integrons among Pseudomonas aeruginosa and Acinetobacter baumannii isolates from patients in Nanjing, China. J Clin Microbiol 45(1):241–243 · DOI
  13. Huovinen P (2001) Resistance to trimethoprim-sulfamethoxazole. Clin Infect Dis 32(11):1608–1614 · DOI · Paper page
  14. Kim MJ, Bae IK, Jeong SH, Kim SH, Song JH, Choi JY, Yoon SS, Thamlikitkul V, Hsueh P-R, Yasin RM et al (2013) Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries. J Antimicrob Chemother 68(12):2820–2824 · DOI
  15. Labarca JA, Salles MJ, Seas C, Guzman-Blanco M (2016) Carbapenem resistance in Pseudomonas aeruginosa and Acinetobacter baumannii in the nosocomial setting in Latin America. Crit Rev Microbiol 42(2):276–292
  16. Lee YC, Ahn BJ, Jin JS, Kim JU, Lee SH, Song DY, Lee WK, Lee JC (2007) Molecular characterization of Pseudomonas aeruginosa isolates resistant to all antimicrobial agents, but susceptible to colistin, in Daegu, Korea. J Microbiol 45(4):358–363
  17. Levesque C, Piche L, Larose C, Roy PH (1995) PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob Agents Chemother 39(1):185–191 · DOI
  18. Levings RS, Lightfoot D, Elbourne LD, Djordjevic SP, Hall RM (2006) New integron-associated gene cassette encoding a trimethoprim-resistant DfrB-type dihydrofolate reductase. Antimicrob Agents Chemother 50(8):2863–2865 · DOI
  19. Liakopoulos A, Mavroidi A, Katsifas EA, Theodosiou A, Karagouni AD, Miriagou V, Petinaki E (2013) Carbapenemase-producing Pseudomonas aeruginosa from central Greece: molecular epidemiology and genetic analysis of class I integrons. BMC Infect Dis 13:505 · DOI
  20. Libisch B, Gacs M, Csiszár K, Muzslay M, Rókusz L, Füzi M (2004) Isolation of an integron-borne bla(VIM-4) type metallo-β-lactamase gene from a carbapenem-resistant Pseudomonas aeruginosa clinical isolate in Hungary. Antimicrob Agents Chemother 48(9):3576–3578 · DOI
  21. Ma Z, Zhou L, Wang H, Luo L (2015) Investigation on the genomic diversity of OXA from isolated Acinetobacter baumannii. Int J Clin Exp Med 8(3):4429–4432
  22. Manenzhe RI, Zar HJ, Nicol MP, Kaba M (2015) The spread of carbapenemase-producing bacteria in Africa: a systematic review. J Antimicrob Chemother 70(1):23–40 · DOI
  23. Manikal VM, Landman D, Saurina G, Oydna E, Lal H, Quale J (2000) Endemic carbapenem-resistant Acinetobacter species in Brooklyn, New York: citywide prevalence, interinstitutional spread, and relation to antibiotic usage. Clin Infect Dis 31(1):101–106 · DOI
  24. Miriagou V, Cornaglia G, Edelstein M, Galani I, Giske CG, Gniadkowski M, Malamou-Lada E, Martinez-Martinez L, Navarro F, Nordmann P et al (2010) Acquired carbapenemases in Gram-negative bacterial pathogens: detection and surveillance issues. Clin Microbiol Infect 16(2):112–122 · DOI
  25. Mushi MF, Mshana SE, Imirzalioglu C, Bwanga F (2014) Carbapenemase genes among multidrug resistant gram negative clinical isolates from a tertiary hospital in Mwanza, Tanzania. BioMed Res Int 2014:303104 · DOI
  26. Okoche D, Asiimwe BB, Katabazi FA, Kato L, Najjuka CF (2015) Prevalence and characterization of carbapenem-resistant Enterobacteriaceae isolated from Mulago National Referral Hospital, Uganda. PLoS ONE 10(8):e0135745 · DOI
  27. Papp-Wallace KM, Endimiani A, Taracila MA, Bonomo RA (2011) Carbapenems: past, present, and future. Antimicrob Agents Chemother 55(11):4943–4960 · DOI
  28. Paulsen IT, Littlejohn TG, Rådström P, Sundström L, Sköld O, Swedberg G, Skurray RA (1993) The 3′ conserved segment of integrons contains a gene associated with multidrug resistance to antiseptics and disinfectants. Antimicrob Agents Chemother 37(4):761–768 · DOI
  29. Pitout JD, Gregson DB, Poirel L, McClure JA, Le P, Church DL (2005) Detection of Pseudomonas aeruginosa producing metallo-beta-lactamases in a large centralized laboratory. J Clin Microbiol 43(7):3129–3135 · DOI
  30. Pitout JD, Revathi G, Chow BL, Kabera B, Kariuki S, Nordmann P, Poirel L (2008) Metallo-beta-lactamase-producing Pseudomonas aeruginosa isolated from a large tertiary centre in Kenya. Clin Microbiol Infect 14(8):755–759 · DOI
  31. Queenan AM, Bush K (2007) Carbapenemases: the versatile beta-lactamases. Clin Microbiol Rev 20(3):440–458 (table of contents) · DOI
  32. Sabtcheva S, Galimand M, Gerbaud G, Courvalin P, Lambert T (2003) Aminoglycoside resistance gene ant(4′)-IIb of Pseudomonas aeruginosa BM4492, a clinical isolate from Bulgaria. Antimicrob Agents Chemother 47(5):1584–1588 · DOI
  33. Seni J, Najjuka CF, Kateete DP, Makobore P, Joloba ML, Kajumbula H, Kapesa A, Bwanga F (2013) Antimicrobial resistance in hospitalized surgical patients: a silently emerging public health concern in Uganda. BMC Res Notes 6:298 · DOI
  34. Tam VH, Chang KT, Abdelraouf K, Brioso CG, Ameka M, McCaskey LA, Weston JS, Caeiro JP, Garey KW (2010) Prevalence, resistance mechanisms, and susceptibility of multidrug-resistant bloodstream isolates of Pseudomonas aeruginosa. Antimicrob Agents Chemother 54(3):1160–1164 · DOI
  35. Tenover FC (2006) Mechanisms of antimicrobial resistance in bacteria. Am J Med 119(6 Suppl 1):S3–S10 (discussion S62–S70) · DOI
  36. Tsakris A, Ikonomidis A, Pournaras S, Tzouvelekis LS, Sofianou D, Legakis NJ, Maniatis AN (2006) VIM-1 metallo-beta-lactamase in Acinetobacter baumannii. Emerg Infect Dis 12(6):981–983 · DOI
  37. Turton JF, Kaufmann ME, Glover J, Coelho JM, Warner M, Pike R, Pitt TL (2005) Detection and typing of integrons in epidemic strains of Acinetobacter baumannii found in the United Kingdom. J Clin Microbiol 43(7):3074–3082 · DOI
  38. Turton JF, Woodford N, Glover J, Yarde S, Kaufmann ME, Pitt TL (2006) Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species. J Clin Microbiol 44(8):2974–2976 · DOI
  39. Uma Karthika R, Srinivasa Rao R, Sahoo S, Shashikala P, Kanungo R, Jayachandran S, Prashanth K (2009) Phenotypic and genotypic assays for detecting the prevalence of metallo-beta-lactamases in clinical isolates of Acinetobacter baumannii from a South Indian tertiary care hospital. J Med Microbiol 58(Pt 4):430–435 · DOI
  40. William S, Feil H (2012) Bacterial genomic DNA isolation using CTAB. Sigma 50:6876
  41. Woodford N, Eastaway AT, Ford M, Leanord A, Keane C, Quayle RM, Steer JA, Zhang J, Livermore DM (2010) Comparison of BD Phoenix, Vitek 2, and MicroScan automated systems for detection and inference of mechanisms responsible for carbapenem resistance in Enterobacteriaceae. J Clin Microbiol 48(8):2999–3002 · DOI
  42. Yamamoto M, Nagao M, Matsumura Y, Matsushima A, Ito Y, Takakura S, Ichiyama S (2011) Interspecies dissemination of a novel class 1 integron carrying blaIMP-19 among Acinetobacter species in Japan. J Antimicrob Chemother 66(11):2480–2483 · DOI

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Metadata

Authors
Kateete, D. P., Nakanjako, R., Namugenyi, J., Erume, J., Joloba, M. L., & Najjuka, C. F.
Affiliations
  • Makerere University (UG)
  • Johns Hopkins University (US)
Corresponding author
Najjuka, Christine F.
ORCID
Venue
SpringerPlus
Publisher
Springer Science and Business Media LLC
Published
2016-08-09
Volume
5
Issue
1
Pages
1308-1308
ISSN
2193-1801 (ISSN-L)
DOI
10.1186/s40064-016-2986-7
PMC
PMC4978656
OpenAlex
W2517313720
Cited by
84
References
0
Citations by year
2026: 6 · 2025: 5 · 2024: 8 · 2023: 12 · 2022: 9 · 2021: 13 · 2020: 8 · 2019: 9 · 2018: 10 · 2017: 4
FWCI
3.74
Type
journal article
Language
en
Open access
Licence
Locations
  • SpringerPlus (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Ministry of Health, Uganda
  • University of Health and Allied Sciences
  • National Institute of Allergy and Infectious Diseases (R01AI075637, R03AI062849)
Topics
  • Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
  • Health Sciences › Medicine › Infectious Diseases › Antibiotic Use and Resistance
  • Health Sciences › Medicine › Microbiology (medical) › Infections and bacterial resistance
Keywords
carbapenem resistance, Pseudomonas aeruginosa, Acinetobacter baumannii, carbapenemase genes, class 1 integron, hospital environmental contamination, nosocomial infections, multidrug resistance, Gram-negative bacteria, Mulago Hospital, Uganda, prevalence, antimicrobial susceptibility

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CoChat. (2026). Citation check: Carbapenem resistant Pseudomonas aeruginosa and Acinetobacter baumannii at Mulago Hospital in Kampala, Uganda (2007–2009). https://app.cochat.ai/papers/10.1186/s40064-016-2986-7
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Kateete, D. P., Nakanjako, R., Namugenyi, J., Erume, J., Joloba, M. L., & Najjuka, C. F. (2016). Carbapenem resistant Pseudomonas aeruginosa and Acinetobacter baumannii at Mulago Hospital in Kampala, Uganda (2007–2009). SpringerPlus. https://doi.org/10.1186/s40064-016-2986-7