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Cited by 61Enzyme-mediated dye degradationAdsorption and biosorption for pollutant removalLignin and Wood Chemistry

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Studies on the laccase-mediated decolorization, kinetic, and microtoxicity of some synthetic azo dyes

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Forootanfar, Hamid, Rezaei, Shahla, Zeinvand-Lorestani, Hamed, Tahmasbi, Hamed, Mogharabi, Mehdi, Ameri, Alieh, Faramarzi, Mohammad Ali·2016·Journal of Environmental Health Science and Engineering
DOI 10.1186/s40201-016-0248-9PMC PMC4866392
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Abstract

BACKGROUND: Enzymatic elimination of synthetic dyes, one of the most environmentally hazardous chemicals, has gained a great interest during the two last decades. The present study was performed to evaluate the decolorization and detoxification potential of the purified laccase of Paraconiothyrium variabile in both non-assisted and hydroxybenzotriazole-aided form against six azo dyes. RESULTS: The obtained results showed that Acid Orange 67, Disperse Yellow 79, Basic Yellow 28, Basic Red 18, Direct Yellow 107, and Direct Black 166 were decolorized up to 65.3, 53.3, 46.7, 40.7, 34, and 26.2 %, respectively, after 1 h treatment with laccase (0.5 U/mL). Addition of HBT up to 5 mM enhanced decolorization percent of all the investigated dyes. The results of kinetic study introduced the monoazo dye of Acid Orange 67 as the most suitable substrate for laccase with K m of 0.49 mM and V max of 189 mmol/min/mg. Evaluation the toxic effect of laccase-treated dye sample based on the growth inhibition of standard bacterial strains revealed decrease in toxicity of all applied dyes after treatment by laccase. CONCLUSIONS: Application of the P. variabile laccase as biocatalyst efficiently decreased the toxicity of all studied synthetic azo dyes.

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Fig. 1 Chemical structures of azo dyes applied in the present study
Fig. 1 Chemical structures of azo dyes applied in the present studyCC BY · PubMed Central
Fig. 2 Laccase mediated (0.5 U/mL) decolorization profiles of applied dyes during 180 min of incubation at 35 °C
Fig. 2 Laccase mediated (0.5 U/mL) decolorization profiles of applied dyes during 180 min of incubation at 35 °CCC BY · PubMed Central
Fig. 3 Effect of various (a) pH, and (b) temperatures on decolorization of azo dyes using the purified laccase (0.5 U/mL) after 60 min of incubation. Obtained means were analyzed by ANOVA with Dunnett’s T3 post hoc test (*, p-value <0.05)
Fig. 3 Effect of various (a) pH, and (b) temperatures on decolorization of azo dyes using the purified laccase (0.5 U/mL) after 60 min of incubation. Obtained means were analyzed by ANOVA with Dunnett’s T3 post hoc test (*, p-value <0.05)CC BY · PubMed Central
Fig. 4 Influence of (a) laccase activity and (b) HBT concentration on decolorization of investigated dyes after 60 min of incubation at 35 °C. Significant values (*, p-value <0.05) were achieved after ANOVA analysis with Dunnett’s T3 post hoc test
Fig. 4 Influence of (a) laccase activity and (b) HBT concentration on decolorization of investigated dyes after 60 min of incubation at 35 °C. Significant values (*, p-value <0.05) were achieved after ANOVA analysis with Dunnett’s T3 post hoc testCC BY · PubMed Central

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Research works printed but not linked: 42

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Authors
Forootanfar, H., Rezaei, S., Zeinvand-Lorestani, H., Tahmasbi, H., Mogharabi, M., Ameri, A., & Faramarzi, M. A.
Affiliations
  • Kerman University of Medical Sciences (IR)
  • Tehran University of Medical Sciences (IR)
Corresponding author
Faramarzi, Mohammad Ali
ORCID
Venue
Journal of Environmental Health Science and Engineering
Publisher
Springer Science and Business Media LLC
Published
2016-05-13
Volume
14
Issue
1
Pages
7-7
ISSN
  • 1735-1979 (ISSN-L)
  • 2052-336X
DOI
10.1186/s40201-016-0248-9
PMC
PMC4866392
OpenAlex
W2375758357
Cited by
61
References
0
Citations by year
2026: 4 · 2025: 6 · 2024: 8 · 2023: 6 · 2022: 6 · 2021: 5 · 2020: 4 · 2019: 7 · 2018: 10 · 2017: 4 · 2016: 1
FWCI
8.59
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Environmental Health Science and Engineering (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
Tehran University of Medical Sciences and Health Services
Topics
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Enzyme-mediated dye degradation
  • Physical Sciences › Environmental Science › Water Science and Technology › Adsorption and biosorption for pollutant removal
  • Physical Sciences › Chemical Engineering › Process Chemistry and Technology › Lignin and Wood Chemistry
Keywords
laccase, azo dyes, dye decolorization, hydroxybenzotriazole, enzyme kinetics, bacterial growth inhibition, Basic Yellow 28, dye detoxification

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