Antimicrobial Susceptibility Testing Protocols
Abstract
The clinical microbiology laboratory is often a sentinel for the detection of drug resistant strains of microorganisms. Standardized protocols require continual scrutiny to detect emerging phenotypic resistance patterns. The timely notification of clinicians with susceptibility results can initiate the alteration of antimicrobial chemotherapy and
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In disk diffusion, glycopeptide antibiotics like vancomycin have limited diffusion due to large molecular weight, leading to unreliable results.
“glycopeptide antibiotics, like vancomycin, have a large molecular weight which hinders their diffusion in agar. This limited diffusion and poorly defined concentration gradient around a vancomycin disk can lead to only a slight difference in zone size between susceptible and resistant strains, potentially resulting in unreliable results.”
From Methods for screening and evaluation of antimicrobial activity: A review of protocols, advantages, and limitations (2024) · cites this paper as [73]
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Hossain, T. J. (2024) · European Journal of Microbiology and Immunology · doi:10.1556/1886.2024.00035 · Cited by 392 · see citing works
Kapadia, S., Pudakalkatti, P., & Shivanaikar, S. (2015) · Contemporary Clinical Dentistry · doi:10.4103/0976-237x.169864 · Cited by 121 · see citing works
Fernandez, B., Vimont, A., Desfossés-Foucault, É., Daga, M., Arora, G., & Fliss, I. (2017) · Food Control · doi:10.1016/j.foodcont.2017.03.007 · Cited by 91 · see citing works
- Authors
- Schwalbe, R. S., Steele‐Moore, L., & Goodwin, A. C.
- Affiliations
- Christiana Care Health System (US)
- Venue
- CRC Press eBooks
- Publisher
- CRC Press
- Published
- 2007-05-22
- OpenAlex
- W567237283
- Cited by
- 576
- References
- 0
- Citations by year
- 2026: 14 · 2025: 25 · 2024: 41 · 2023: 42 · 2022: 54 · 2021: 45 · 2020: 42 · 2019: 31 · 2018: 46 · 2017: 40 · 2016: 36 · 2015: 38 · 2014: 22 · 2013: 36 · 2012: 24
- Type
- reference entry
- Language
- en
- Open access
- closed
- Locations
- CRC Press eBooks (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antibiotic Use and Resistance
- Keywords
- antimicrobial susceptibility testing, antimicrobial resistance, drug-resistant microorganisms, standardized testing protocols, clinical microbiology laboratory
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