CoChat
Sign Up
Cited by 108Monoclonal and Polyclonal Antibodies ResearchBarrier Structure and Function StudiesDrug Transport and Resistance Mechanisms

The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.

Investigation of the Influence of FcRn on the Distribution of IgG to the Brain

Open access
Garg, Amit, Balthasar, Joseph P.·2009·The AAPS Journal
DOI 10.1208/s12248-009-9129-9PMC PMC2758122
Open this paper in CoChat — free accountOpen PDF

Abstract

It has been suggested that the neonatal Fc receptor (FcRn) is a primary determinant of the distribution of IgG to the brain. In the present report, (125)I-labeled 7E3, a monoclonal IgG1 antibody, was injected intravenously to groups of FcRn-deficient mice and C57BL/6J control mice. Sub-groups of three mice were sacrificed at several time points. Blood and brain tissue were harvested and radioactivity was assessed. Antibody concentrations in brain were corrected for residual blood using (51)Cr-labeled red blood cells. Data were analyzed via WinNonlin, and areas under plasma and tissue concentration vs. time curves (AUCs) were assessed via the Bailer method. The apparent plasma elimination half-life and clearance of 7E3 were 13.61 +/- 0.61 days and 6.5 +/- 0.10 ml/day/kg in control mice and 0.70 +/- 0.05 days and 63.5 +/- 2.7 ml/day/kg in the knockout mice. Plasma and brain AUCs (0-10 days) were found to be 3,338.7 +/- 50.4 and 7.46 +/- 0.5 nM day in control animals and 781.2 +/- 16.6 and 1.65 +/- 0.1 nM day in FcRn-deficient animals. There was no significant difference between brain-to-plasma AUC ratios in control and FcRn-deficient mice (0.0022 +/- 0.00015 vs. 0.0021 +/- 0.00011, p = 0.3347). Two-way analysis of variance showed no significant differences, at any time point, between brain-to-plasma concentration ratios determined from control and knockout animals. The results suggest that FcRn does not contribute significantly to the "blood-brain barrier" for IgG in mice, and the data suggest that FcRn is not responsible for the low exposure of IgG in the brain relative to plasma.

Overview

Integrity signals

Registry

Crossref · 10.1208/s12248-009-9129-9

Cites

42 of 42 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Cited by

Sorted by citations

See all 108

Related

See all

Metadata

Authors
Garg, A., & Balthasar, J. P.
Affiliations
University at Buffalo, State University of New York (US)
Corresponding author
Balthasar, Joseph P.
ORCID
Venue
The AAPS Journal
Publisher
Springer Science and Business Media LLC
Published
2009-07-28
Volume
11
Issue
3
Pages
553-7
ISSN
1550-7416 (ISSN-L)
DOI
10.1208/s12248-009-9129-9
PMC
PMC2758122
OpenAlex
W2045301605
Cited by
108
References
42
Citations by year
2026: 3 · 2025: 8 · 2024: 9 · 2023: 5 · 2022: 9 · 2021: 9 · 2020: 7 · 2019: 5 · 2018: 11 · 2017: 3 · 2016: 7 · 2015: 5 · 2014: 6 · 2013: 6 · 2012: 8
FWCI
3.78
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • The AAPS Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Eli Lilly and Company
  • National Institutes of Health
  • National Institute of Allergy and Infectious Diseases (AI60687, R01AI060687)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Monoclonal and Polyclonal Antibodies Research
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords
neonatal Fc receptor, IgG, brain distribution, blood-brain barrier, antibody clearance, plasma half-life, brain-to-plasma ratio, passive immunization
MeSH terms
Animals, beta 2-Microglobulin, Brain, Immunoglobulin G, Iodine Radioisotopes, Mice, Inbred C57BL, Receptors, Fc, Histocompatibility Antigens Class I, Mice, Knockout, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review