The DOI matches its OpenAlex record; 40 of the 40 references it cites were found in a registry or an index.
Cytokines Induce Deoxyribonucleic Acid Strand Breaks and Apoptosis in Human Pancreatic Islet Cells
Open accessAbstract
We have previously observed that a 6-day exposure of human pancreatic islets to a combination of cytokines (interleukin-1beta 50 U/ml + tumour necrosis factor-alpha 1000 U/ml + interferon-gamma 1000 U/ml) severely impairs beta-cell functions. In the present study, we examined whether this condition affects DNA integrity and viability of human islet cells. Cells were studied after 3, 6, and 9 days of cytokine treatment by both single cell gel electrophoresis (the "comet assay," a sensitive method for detection of DNA strand breaks) and by a cytotoxicity assay using the DNA binding dyes Hoechst 33342 and propidium iodide as indices for the number of viable, necrotic, and apoptotic cells. Cytokine treatment for 6 and 9 days resulted in a 50% increase in comet length (P < 0.01 vs. controls), indicating DNA strand breaks, as well as in a significant increase in the number of apoptotic cells (P < 0.02 vs. controls), but not in the number of necrotic cells. The arginine analogs N(G)-nitro-L-arginine and N(G)-monomethyl-L-arginine prevented nitric oxide formation by the cytokines but did not interfere with cytokine-induced DNA strand breaks and apoptosis. The present data suggest that prolonged (6-9 days) exposure of human pancreatic islets to a mixture of cytokines induces DNA strand breaks and cell death by apoptosis. These deleterious effects of cytokines appear to be independent of nitric oxide generation.
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Cites
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Cited by
Nitric Oxide and Peroxynitrite in Health and Disease
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A choice of death - the signal-transduction of immune-mediated beta-cell apoptosis
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Self-citationGlucagon-like Peptide-1 Receptor Signaling Modulates β Cell Apoptosis
Li, Y., Hansotia, T., Yusta, B., Ris, F., Halban, P. A., & Drucker, D. J. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m209423200 · Cited by 589
Kaneto, H., Xu, G., Fujii, N., Kim, S., Bonner-Weir, S., & Weir, G. C. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m202066200 · Cited by 349
Cardozo, A. K., Heimberg, H., Heremans, Y., Leeman, R., Kutlu, B., Kruhøffer, M., Ørntoft, T., & Eizirik, D. L. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m108658200 · Cited by 301
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Metadata
- Authors
- Delaney, C. A., Pavlović, D., Hoorens, A., PIPELEERS, D. G., & Eizirik, D. L.
- Venue
- Endocrinology
- Publisher
- Oxford University Press
- Published
- 1997-06-01
- Volume
- 138
- Issue
- 6
- Pages
- 2610-2614
- ISSN
- 0013-7227 (ISSN-L)
- OpenAlex
- W2161993072
- Cited by
- 320
- References
- 40
- FWCI
- 14.92
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Cytokines Induce Deoxyribonucleic Acid Strand Breaks and Apoptosis in Human Pancreatic Islet Cells. https://app.cochat.ai/papers/10.1210/en.138.6.2610
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- Delaney, C. A., Pavlović, D., Hoorens, A., PIPELEERS, D. G., & Eizirik, D. L. (1997). Cytokines Induce Deoxyribonucleic Acid Strand Breaks and Apoptosis in Human Pancreatic Islet Cells. Endocrinology. https://doi.org/10.1210/en.138.6.2610
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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