Impaired preadipocyte differentiation into adipocytes in subcutaneous abdominal adipose of PCOS-like female rhesus monkeys
Open accessAbstract
Metabolic characteristics of polycystic ovary syndrome women and polycystic ovary syndrome-like, prenatally androgenized (PA) female monkeys worsen with age, with altered adipogenesis of sc abdominal adipose potentially contributing to age-related adverse effects on metabolism. This study examines whether adipocyte morphology and gene expression in sc abdominal adipose differ between late reproductive-aged PA female rhesus monkeys compared with age-matched controls (C). Subcutaneous abdominal adipose of both groups was obtained for histological imaging and mRNA determination of zinc finger protein 423 (Zfp423) as a marker of adipose stem cell commitment to preadipocytes, and CCAAT/enhancer binding protein (C/EBP)α/peroxisome proliferator-activated receptor (PPAR)δ as well as C/EBPα/PPARγ as respective markers of early- and late-stage differentiation of preadipocytes to adipocytes. In all females combined, serum testosterone (T) levels positively correlated with fasting serum levels of total free fatty acid (r(2) = 0.73, P < .002). PA females had a greater population of small adipocytes vs C (P < .001) in the presence of increased Zfp423 (P < .025 vs C females) and decreased C/EBPα (P < .003, vs C females) mRNA expression. Moreover, Zfp423 mRNA expression positively correlated with circulating total free fatty acid levels during iv glucose tolerance testing (P < .004, r(2) = 0.66), whereas C/EBPα mRNA expression negatively correlated with serum T levels (P < .02, r(2) = 0.43). Gene expression of PPARδ and PPARγ were comparable between groups (P = .723 and P = .18, respectively). Early-to-mid gestational T excess in female rhesus monkeys impairs adult preadipocyte differentiation to adipocytes in sc abdominal adipose and may constrain the ability of this adipose depot to safely store fat with age.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Impaired adipocyte differentiation has been demonstrated in nonhuman primate models.
“Impaired adipocyte differentiation has been demonstrated in NHP models [105].”
From Polycystic Ovary Syndrome: Pathophysiology, Presentation, and Treatment With Emphasis on Adolescent Girls (2019) · cites this paper as [105]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 53 · Cited by 60
Research works printed but not linked: 2
- [25]25. Goy RW, Robinson JA. Prenatal exposure of rhesus monkeys to patent androgens: morphological, behavioral, and physiological consequences. In: Hunt VR, Smith MK, Worth D, eds. Banbury Report II: Environmental Factors in Human Growth and Development. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1982:355–378 [Google Scholar]
- [45]45. Macut D, Panidis D, Glisi B, et al. Lipid and lipoprotein profile in women with polycystic ovary syndrome. Can J Physiol Pharmacol. 2008;86:199–204 [DOI] [PubMed] [Google Scholar]
- Authors
- Keller, E., Chazenbalk, G. D., Aguilera, P., Madrigal, V., Grogan, T., Elashoff, D., Dumesic, D. A., & Abbott, D. H.
- Affiliations
- University of California, Los Angeles (US)
- University of Wisconsin–Madison (US)
- Corresponding author
- Chazenbalk, Gregorio D.
- ORCID
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2014-07-01
- Volume
- 155
- Issue
- 7
- Pages
- 2696-2703
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- PMC
- PMC4060192
- OpenAlex
- W2153257786
- Cited by
- 60
- References
- 53
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 1 · 2020: 9 · 2019: 10 · 2018: 8 · 2017: 7 · 2016: 6 · 2015: 3 · 2014: 1
- FWCI
- 6.88
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Endocrinology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5u01hd044650-05, UL1TR000124, P51OD011106)
- National Center for Research Resources (R01RR013635, P51RR000167)
- National Center for Advancing Translational Sciences
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (P50HD071836, U01-HD044650, U54HD071836)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- polycystic ovary syndrome, prenatal androgen exposure, rhesus monkeys, subcutaneous abdominal fat, preadipocyte differentiation, adipogenesis, Zfp423, C/EBPα, PPARδ, PPARγ, testosterone, free fatty acids, adipocyte size, aging, glucose tolerance
- MeSH terms
- Androgens, Animals, Cell Differentiation, Female, Humans, Macaca mulatta, Polycystic Ovary Syndrome, Testosterone, Transcription Factors, Gene Expression, Zinc Fingers, Adipocytes, Reverse Transcriptase Polymerase Chain Reaction, CCAAT-Enhancer-Binding Protein-alpha, PPAR delta, PPAR gamma, Adipogenesis, Subcutaneous Fat, Abdominal
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
