The DOI matches its Crossref record.
Interplay of Nutrients and Hormones in the Regulation of Glucagon Release*
Abstract
The role of nutrients and hormones in the regulation of glucagon release is investigated in pancreatic A cells purified by autofluorescence-activated cell sorting. Purified A cells lack secretory activity in 1-h incubation at 1.4 mM glucose. Their release mechanism can be activated by arginine, alanine, and glutamine, alone or in combination. Glucose inhibits amino acid-induced glucagon release through a direct insulin-independent action upon pancreatic A cells. Nutrient-induced glucagon release is suppressed by somatostatin and amplified by (Bu)2cAMP or epinephrine. The epinephrine stimulus is inhibited by 10(-11) M somatostatin and abolished by 10(-10) M of this peptide. The effects of somatostatin and epinephrine are associated with parallel changes in cellular cAMP levels, which is not the case for the variations induced by amino acids or glucose. It is confirmed that calcium is an essential requirement for glucagon release. In contrast to its exquisite sensitivity for somatostatin, the glucagon release process is relatively insensitive to insulin during a 1-h exposure. The hormone affects solely epinephrine-induced glucagon release and its inhibitory action is partial and only observed at 10(-7) M. This suppressive effect of insulin is not attributable to variations in glucose handling but appears associated with the stimulatory effect of epinephrine. It is concluded that a nutrient-induced signal interacts with a hormone-inducible cAMP signal to activate the secretory process in pancreatic A cells.
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“and studies of pancreatic islets and cells have provided additional evidence that glucose regulates glucagon release by a direct effect on the alpha cell [5,[8][9][10][12][13][14][15][16][17].”
From Glucose inhibits glucagon secretion by a direct effect on mouse pancreatic alpha cells (2007) · cites this paper as [8]
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The role of incretins in glucose homeostasis and diabetes treatment
Kim, W., & Egan, J. M. (2008) · Pharmacological Reviews · doi:10.1124/pr.108.000604 · Cited by 824
“, 1996) and compatible with this observation, exposure of cells to GIP increases cAMP content to levels that are required for nutrient-induced glucagon secretion (Pipeleers et al., 1985; Moens et al., 1996), and GIP stimulates Ca(2) induced exocytosis in isolated rat cells by a PKAmediated mechanism (Ding and Gromada,…”
Backgroundα-Cells of the Endocrine Pancreas: 35 Years of Research but the Enigma Remains
Gromada, J., Franklin, I., & Wollheim, C. B. (2007) · Endocrine Reviews · doi:10.1210/er.2006-0007 · Cited by 596
Glucose-inhibition of glucagon secretion involves activation of GABAA-receptor chloride channels
Rorsman, P., Berggren, P., Bokvist, K. B., Ericson, H., Möhler, H., Östenson, C., & Smith, P. A. (1989) · Nature · doi:10.1038/341233a0 · Cited by 463
Yusta, B., Baggio, L. L., Estall, J. L., Koehler, J. A., Holland, D. P., Li, H., Pipeleers, D., Ling, Z., & Drucker, D. J. (2006) · Cell Metabolism · doi:10.1016/j.cmet.2006.10.001 · Cited by 454
Self-citationPhysiology of the pancreatic α-cell and glucagon secretion: role in glucose homeostasis and diabetes
Quesada, I., Tudurí, E., Ripoll, C., & Nadal, Á. (2008) · Journal of Endocrinology · doi:10.1677/joe-08-0290 · Cited by 447
Metadata
- Authors
- PIPELEERS, D. G., SCHUIT, F. C., SCHRAVENDIJK, C. F. H. V., & WINKEL, M. V. D.
- Affiliations
- Vrije Universiteit Brussel (BE)
- ORCID
- PIPELEERS, D. G.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1985-09
- Volume
- 117
- Issue
- 3
- Pages
- 817-823
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W4250756743
- Cited by
- 169
- References
- 0
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 1 · 2020: 1 · 2019: 7 · 2018: 6 · 2017: 2 · 2016: 5 · 2014: 5 · 2013: 2 · 2012: 5
- FWCI
- 6.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- VUBIR (Vrije Universiteit Brussel) (submittedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- glucagon secretion, pancreatic A cells, nutrient regulation, amino acids, glucose, somatostatin, epinephrine, cyclic AMP, insulin, calcium, pancreatic islets
- MeSH terms
- Alanine, Amino Acids, Animals, Arginine, Calcium, Bucladesine, Epinephrine, Flow Cytometry, Glucagon, Glucose, Glutamine, Islets of Langerhans, Male, Pancreatic Hormones, Rats, Inbred Strains, Somatostatin, Rats, In Vitro Techniques
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