Characterization of Insulin-Like Growth Factor I and Insulin Receptors on Cultured Bovine Adrenal Fasciculata Cells. Role of these Peptides on Adrenal Cell Function*

PENHOAT, A.; CHATELAIN, P. G.; JAILLARD, C.; SAEZ, J. M.

doi:10.1210/endo-122-6-2518

Characterization of Insulin-Like Growth Factor I and Insulin Receptors on Cultured Bovine Adrenal Fasciculata Cells. Role of these Peptides on Adrenal Cell Function*

We have characterized insulin-like growth factor I (IGF-I) and insulin receptors in cultured bovine adrenal cells by binding and cross-linking affinity experiments. At equilibrium the dissociation constant and the number of binding sites per cell for IGF-I were 1.4 +/- (SE) 0.3 x 10(-9) M and 19,…