The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Cortisol inhibits the synthesis of insulin-like growth factor-I in skeletal cells.
Abstract
Supraphysiological levels of cortisol inhibit bone cell replication and matrix synthesis, but its mechanism of action is unknown and could be secondary to an inhibition of local growth factor synthesis. These inhibitory effects of cortisol are the converse of the observed anabolic influences of the endogenously produced insulin-like growth factor-I (IGF-I); therefore, cortisol was examined for its effect on the production of IGF-I in osteoblast (Ob)- and fibroblast/preosteoblast-enriched cell cultures prepared from fetal rat parietal bone. Synthesis of IGF-I was monitored by Northern blot analysis to determine steady state IGF-I mRNA levels and by an IGF-I-specific RIA to quantitate polypeptide levels in acidified and fractionated culture medium. Cortisol at 100 nM decreased IGF-I transcript levels by 60% or more in Ob cultures within 6 h of treatment, and the concentration of immunoreactive IGF-I by 50% after 24 h; these effects were observed in the absence of a change in cellular DNA content. In Ob cultures, PTH at 10 nM increased IGF-I transcripts at 6 h and polypeptide levels at 24 h by 2.5- and 4.1-fold, respectively, and cortisol opposed this effect. The inhibitory effect of cortisol was not specific for the Ob cell population, since at 100 nM it also decreased IGF-I transcript and immunoreactive IGF-I levels in fibroblast/preosteoblast cultures and opposed the stimulation of IGF-I synthesis after treatment with 100 ng/ml GH. In conclusion, high levels of cortisol decrease skeletal IGF-I synthesis by reducing IGF-I transcript levels, and this effect probably contributes to the inhibitory influence of cortisol on bone formation.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Glucocorticoids can also inhibit osteoblast differentiation (249 -251), osteoblast attachment to bone matrix (252), and osteoblast production of IGF-I (253).”
From Receptor Activator of Nuclear Factor κB Ligand and Osteoprotegerin Regulation of Bone Remodeling in Health and Disease (2008) · cites this paper as [253]
Citations
Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,512
Kissane, J. M., & Robins, E. (1958) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)68051-8 · Cited by 1,774
Growth factors and the regulation of bone remodeling.
CANALIS, E. M., & McCarthy, T. L. (1988) · Journal of Clinical Investigation · doi:10.1172/jci113318 · Cited by 608
Self-citationInsulin-Like Growth Factor I Has Independent Effects on Bone Matrix Formation and Cell Replication*
Hock, J. M., & CANALIS, E. M. (1988) · Endocrinology · doi:10.1210/endo-122-1-254 · Cited by 600
Self-citationCANALIS, E. M., Burch, W. M., & McCarthy, T. L. (1989) · Journal of Clinical Investigation · doi:10.1172/jci113885 · Cited by 541
Self-citationMellow, J. S. M. D., & Baxter, R. C. (1988) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(88)80824-6 · Cited by 485
Negative Regulation by Glucocorticoids Through Interference with a cAMP Responsive Enhancer
Akerblom, I. E., Slater, E. P., Beato, M., Baxter, J. D., & Mellon, P. L. (1988) · Science · doi:10.1126/science.2838908 · Cited by 481
McCarthy, T. L., & CANALIS, E. M. (1989) · Endocrinology · doi:10.1210/endo-124-1-301 · Cited by 434
Self-citationWong, G. L., & Cohn, D. V. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.8.3167 · Cited by 354
Cited by
Sorted by citations
Ohlsson, C., Bengtsson, B., Isaksson, O. G. P., Andreassen, T. T., & Slootweg, M. C. (1998) · Endocrine Reviews · doi:10.1210/edrv.19.1.0324 · Cited by 863
Kearns, A. E., Khosla, S., & Kostenuik, P. J. (2008) · Endocrine Reviews · doi:10.1210/er.2007-0014 · Cited by 824
Andress, D. L., & Birnbaum, R. S. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)41695-x · Cited by 343
Animal models for osteoporosis
Komori, T. (2015) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2015.03.028 · Cited by 317
The role of leptin in regulating bone metabolism
Upadhyay, J., Farr, O. M., & Mantzoros, C. S. (2014) · Metabolism · doi:10.1016/j.metabol.2014.10.021 · Cited by 277
“[73] McCarthy TL, Centrella M, Canalis E.”
Metadata
- Authors
- MCCARTHY, T. L., CENTRELLA, M., & CANALIS, E.
- Affiliations
- Saint Francis Hospital & Medical Center (US)
- ORCID
- CANALIS, ERNESTO
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1990-03
- Volume
- 126
- Issue
- 3
- Pages
- 1569-1575
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W1981791036
- Cited by
- 204
- References
- 32
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 3 · 2023: 4 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 3 · 2018: 2 · 2016: 3 · 2015: 5 · 2014: 5 · 2013: 2 · 2012: 3
- FWCI
- 14.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Manitoba
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK042424, DK-42424)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR-39201, AR 21707)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Keywords
- cortisol, insulin-like growth factor I, osteoblasts, parietal bone, parathyroid hormone, growth hormone, IGF-I mRNA, bone formation
- MeSH terms
- Animals, Bone and Bones, Cells, Cultured, DNA, Hydrocortisone, Insulin-Like Growth Factor I, Nucleic Acid Hybridization, Parathyroid Hormone, RNA, Somatomedins, Transcription, Genetic, Blotting, Northern
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
