A Microgel Electrophoretic Analysis of the Colloid Proteins in Single Rat Thyroid Follicles. I. The Qualitative Protein Composition of the Colloid in Normal Thyroids
Abstract
Samples of the colloid in rat thyroid follicles were obtained by micropuncture of superficial follicles in the isthmus in vivo and of superficial and deeper follicles in saline perfused thyroids. The protein composition of the samples was analyzed by microgel electrophoresis on polyacrylamide gels at neutral pH. The protein separation patterns were recorded by microdensitometry after staining of the proteins. Thyroglobulin (TG) and protein(s) migrating more slowly than TG were present in all samples collected in vivo. A large number of samples also contained a fraction migrating at the front (prealbumin). An albuminlike protein was present in 2/3 of the samples and a protein fraction, migrating similarly to a tissue fluid protein, was observed in 1/3 of the samples. The 12S TG subunit was observed in a small number of colloid samples. Based on the presence of the different protein fractions in the colloid, the material was blocked into five qualitatively different groups. (Endocrinology 91: 1288, 1972)
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TG storage in the colloid makes TG highly enriched in the lumen, reaching a concentration as high as nearly 600 mg/ml in the human thyroid gland, and constituting >95% of the colloid protein content.
“This unique storage mechanism makes TG highly enriched in the lumen, reaching a concentration as high as nearly 600 mg/ml in the human thyroid gland, and constituting >95% of the colloid protein content (67, 78–80).”
From Intrathyroidal feedforward and feedback network regulating thyroid hormone synthesis and secretion (2022) · cites this paper as [79]
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Intrathyroidal feedforward and feedback network regulating thyroid hormone synthesis and secretion
Jing, L., & Zhang, Q. (2022) · Frontiers in Endocrinology · doi:10.3389/fendo.2022.992883 · Cited by 61 · see citing works
- Authors
- SMEDS, S.
- Affiliations
- University of Gothenburg (SE)
- Corresponding author
- SMEDS, STAFFAN
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1972-11
- Volume
- 91
- Issue
- 5
- Pages
- 1288-1299
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2138715766
- Cited by
- 19
- References
- 0
- Citations by year
- 2022: 1 · 2013: 1 · 2012: 1
- FWCI
- 0.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Cancer Diagnosis and Treatment
- Health Sciences › Medicine › Surgery › Thyroid and Parathyroid Surgery
- Keywords
- thyroid colloid, thyroglobulin, thyroid follicles, prealbumin, protein composition, rat thyroid, microdensitometry
- MeSH terms
- Albumins, Animals, Colloids, Densitometry, Electrophoresis, Polyacrylamide Gel, Iodine Isotopes, Male, Methods, Proteins, Punctures, Thyroglobulin, Thyroid Gland, Rats
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