Evidence for extrathyroidal formation of 3-iodothyronamine in humans as provided by a novel monoclonal antibody-based chemiluminescent serum immunoassay
Abstract
Context Thyronamines are thyronergic metabolites of thyroid hormones. Lack of reliable and sensitive detection methods for endogenous 3-iodothyronamine (3-T(1)AM) has so far hampered progress in understanding their physiological action and role in endocrine homeostasis or pathophysiology of diseases. Objective We characterized newly generated mouse monoclonal 3-T(1)AM antibodies and established a monoclonal antibody-based chemiluminescence immunoassay as a powerful tool for monitoring 3-T(1)AM levels in investigations addressing altered serum profiles and potential sites of origin and action of 3-T(1)AM in humans. Design and setting Our exploratory study on 3-T(1)AM serum levels in humans measured 3-T(1)AM concentrations in comparison with thyroid hormones. Patients or other participants Thirteen adult healthy subjects, 10 patients with pituitary insufficiency, and 105 thyroid cancer patients participated. Interventions INTERVENTIONS included l-T(4) withdrawal in patients with pituitary insufficiency as well as TSH-suppressive T(4) substitution in thyroid cancer patients. Results 3-T(1)AM was reliably quantified in human serum and stable after storage at room temperature and 4 C overnight as well as after four freeze-thaw cycles. The median serum concentration in healthy subjects was 66 ± 26 nm. 3-T(1)AM was also detected in T(4)-substituted thyroid cancer patients. Although free T(4) and T(3) significantly decreased during T(4) withdrawal, 3-T(1)AM levels remained constant f…
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In the same four human paradigms, no changes in 3-T1AM concentrations were found by monoclonal antibody CLIA.
“[and also no changes in concentrations of 3-T1AM, also determined by monoclonal antibody CLIA (59), were found]”
From 3,5-T2—A Janus-Faced Thyroid Hormone Metabolite Exerts Both Canonical T3-Mimetic Endocrine and Intracrine Hepatic Action (2020) · cites this paper as [59]
The detailed assay methodology using a monoclonal antibody-based chemiluminescence immunoassay has been previously described.
“The detailed assay methodology using a monoclonal antibody-based chemiluminescence immunoassay has been previously described [2,6,7]”
From Changes in Thyroid Metabolites after Liothyronine Administration: A Secondary Analysis of Two Clinical Trials That Incorporated Pharmacokinetic Data (2022) · cites this paper as [7]
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Biogenic amines activate blood leukocytes via trace amine-associated receptors TAAR1 and TAAR2
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Self-citation
- Authors
- Hoefig, C. S., Köhrle, J., Brabant, G., Dixit, K., Yap, B., Strasburger, C. J., & Wu, Z.
- Affiliations
- Charité – Universitätsmedizin Berlin (DE)
- The Christie Hospital (GB)
- Stiftung Charité (DE)
- Venue
- The Journal of Clinical Endocrinology & Metabolism
- Publisher
- The Endocrine Society
- Published
- 2011-06
- Volume
- 96
- Issue
- 6
- Pages
- 1864-1872
- ISSN
- 0021-972X (ISSN-L)
- 1945-7197
- OpenAlex
- W2136133737
- Cited by
- 78
- References
- 1
- Citations by year
- 2024: 2 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 4 · 2019: 9 · 2018: 11 · 2017: 9 · 2016: 11 · 2015: 8 · 2014: 8 · 2013: 5 · 2012: 4
- FWCI
- 5.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Cancer Diagnosis and Treatment
- Health Sciences › Medicine › Surgery › Thyroid and Parathyroid Surgery
- Keywords
- 3-iodothyronamine, thyronamines, monoclonal antibodies, chemiluminescence immunoassay, thyroid hormone withdrawal, thyroid cancer, hypopituitarism, thyroidectomy, radioiodine therapy, thyroid hormone metabolism
- MeSH terms
- Adolescent, Adult, Aged, Aged, 80 and over, Antibodies, Monoclonal, Enzyme-Linked Immunosorbent Assay, Female, Humans, Hypopituitarism, Luminescent Measurements, Male, Middle Aged, Thyroid Hormones, Thyroid Neoplasms, Thyronines
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