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A HIGHLY SENSITIVE IN VITRO BIOASSAY FOR LUTEINIZING HORMONE AND CHORIONIC GONADOTROPIN: TESTOSTERONE PRODUCTION BY DISPERSED LEYDIG CELLS.

Dufau, Maria L., Mendelson, Carole R., Catt, Kevin J.·1974·The Journal of Clinical Endocrinology & Metabolism

Abstract

The in vitro bioassay for LH/hCG employing isolated rat testes has been significantly improved by utilization of collagenase-dispersed interstitial cells in the presence of phosphodiesterase inhibitors. The sensitivity of the assay system has been extended to 20μIU (2 picograms) of hCG, and the index of precision of the method (λ) is less than 0.04. The high sensitivity of the interstitial cell assay permits bioassay of circulating gonadotropins in human plasma.

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Metadata

Authors
Dufau, M. L., Mendelson, C. R., & Catt, K. J.
Venue
The Journal of Clinical Endocrinology & Metabolism
Published
1974-09
Volume
39
Issue
3
Pages
610-613
ISSN
  • 0021-972X (ISSN-L)
  • 1945-7197
OpenAlex
W2015213958
Cited by
267
References
0
FWCI
7.78
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: A HIGHLY SENSITIVE IN VITRO BIOASSAY FOR LUTEINIZING HORMONE AND CHORIONIC GONADOTROPIN: TESTOSTERONE PRODUCTION BY DISPERSED LEYDIG CELLS. https://app.cochat.ai/papers/10.1210/jcem-39-3-610
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Dufau, M. L., Mendelson, C. R., & Catt, K. J. (1974). A HIGHLY SENSITIVE IN VITRO BIOASSAY FOR LUTEINIZING HORMONE AND CHORIONIC GONADOTROPIN: TESTOSTERONE PRODUCTION BY DISPERSED LEYDIG CELLS. The Journal of Clinical Endocrinology & Metabolism. https://doi.org/10.1210/jcem-39-3-610

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.