The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Regulation by Follicle-Stimulating Hormone of the Synthesis of Aromatase Cytochrome P-450 in Human Granulosa Cells
Open accessAbstract
The effects of FSH to increase the activity of aromatase, as well as the synthesis of the components of the aromatase enzyme complex, have been studied in human ovarian granulosa cells obtained from women undergoing oocyte retrieval. FSH increased aromatase activity, as well as the synthesis of aromatase cytochrome P-450 (P-450AROM) in a time-dependent fashion, whereas in the absence of FSH, both activity and synthesis declined with duration of culture. The effect of FSH was mimicked by forskolin, an activator of adenylate cyclase. FSH also increased the synthesis of NADPH-cytochrome P-450 reductase, but to a relatively modest extent. The levels of hybridizable mRNA species encoding cytochrome P-450AROM of lengths 3.0, 2.4, and 1.6 kilobases were also increased with FSH treatment. It is concluded that the regulation of aromatase activity by FSH in human granulosa cells is mediated primarily by changes in the synthesis of cytochrome P-450AROM, that this action of FSH is mediated by cAMP, and that the changes in cytochrome P-450AROM synthesis are the consequences of changes in the levels of mRNA encoding this enzyme.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“It catalyzes the rate-limiting final step in estrogen (E) production, the hydroxylation of androstenedione to estrone and of T to E 2 (Fig. 1).”
From A new era in ovulation induction (2006) · cites this paper as [19]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular Cloning. A Laboratory Manual
Wood, E. (1983) · Biochemical Education · doi:10.1016/0307-4412(83)90068-7 · Cited by 29,534
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,512
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Chirgwin, J. M., Przybyla, A., MacDonald, R. J., & Rutter, W. J. (1979) · Biochemistry · doi:10.1021/bi00591a005 · Cited by 21,460
Cold Spring Harbor Laboratory; Cold Spring Harbor, NY
Cavalier-Smith, T. (1980) · FEBS Letters · doi:10.1016/0014-5793(80)80323-1 · Cited by 6,317
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
(1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90381-6 · Cited by 4,952
Cleveland, D. W., Lopata, M. A., MacDonald, R. J., Cowan, N. J., Rutter, W. J., & Kirschner, M. W. (1980) · Cell · doi:10.1016/0092-8674(80)90238-x · Cited by 1,835
Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells.
Seamon, K. B., Padgett, W., & Daly, J. W. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.6.3363 · Cited by 1,658
The Involvement of Human Placental Microsomal Cytochrome P-450 in Aromatization
Thompson, E. A., & Siiteri, P. K. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)79736-x · Cited by 584
Aromatization of Androstenedione by Human Adipose Tissue Stromal Cells in Monolayer Culture*
Ackerman, G. E., Smith, M. E., Mendelson, C. R., MacDonald, P. C., & Simpson, E. R. (1981) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-53-2-412 · Cited by 389
Self-citationSerum suppresses the expression of hormonally induced functions in cultured granulosa cells
Orly, J., SATO, G. H., & Erickson, G. F. (1980) · Cell · doi:10.1016/0092-8674(80)90328-1 · Cited by 216
Immunochemical Studies on Electron Transport Chains Involving Cytochrome P-450
Masters, B. S. S., Baron, J., Taylor, W. E., Isaacson, E. L., & Lospalluto, J. J. (1971) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)62065-4 · Cited by 211
Garzo, V. G., & Dorrington, J. H. (1984) · American Journal of Obstetrics and Gynecology · doi:10.1016/0002-9378(84)90769-5 · Cited by 194
Kolena, J., & Channing, C. P. (1972) · Endocrinology · doi:10.1210/endo-90-6-1543 · Cited by 172
Mendelson, C. R., Wright, E. E., Evans, C. T., Porter, J. C., & Simpson, E. R. (1985) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(85)90525-9 · Cited by 154
Self-citationIVARIE, R. D., & Jones, P. P. (1979) · Analytical Biochemistry · doi:10.1016/0003-2697(79)90322-1 · Cited by 145
Moon, Y. S., Tsang, B. K., SIMPSON, C., & Armstrong, D. T. (1978) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-47-2-263 · Cited by 144
Erickson, G. F. (1983) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(83)90003-5 · Cited by 123
Evans, C. T., Ledesma, D. B., Schulz, T. Z., Simpson, E. R., & Mendelson, C. R. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.17.6387 · Cited by 86
Self-citationAmino terminal sequence analysis of human placenta aromatase
Chen, S., Shively, J. E., Nakajin, S., Shinoda, M., & Hall, P. F. (1986) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(86)90987-3 · Cited by 56
Trzeciak, W. H., Waterman, M. R., & Simpson, E. R. (1986) · Endocrinology · doi:10.1210/endo-119-1-323 · Cited by 49
Self-citationLH stimulation of estrogen secretion by cultured rat granulosa cells
Wang, C., Hsueh, A. J. W., & Erickson, G. F. (1981) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(81)90075-7 · Cited by 48
Wang, C. E., Hsueh, A. J. W., & Erickson, G. F. (1982) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(82)90170-8 · Cited by 44
Gonadotrophic control of steroidogenesis in human granulosa-lutein cells
Wickings, E. J., Hillier, S. G., & REICHERT, L. E. (1986) · Reproduction · doi:10.1530/jrf.0.0760677 · Cited by 30
Durham, C. R., Zhu, H., Masters, B. S. S., Simpson, E. R., & Mendelson, C. R. (1985) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(85)90177-7 · Cited by 28
Self-citation
Cited by
Sorted by citations
Nishi, Y., Yanase, T., Yi-ming, M., Oba, K., Ichino, I., Saito, M., Nomura, M., Mukasa, C., Okabe, T., Goto, K., et al. (2001) · Endocrinology · doi:10.1210/endo.142.1.7862 · Cited by 596
Steroidogenic enzymes: Structure, function, and role in regulation of steroid hormone biosynthesis
Hanukoglu, I. (1992) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(92)90307-5 · Cited by 577
Groh, K. J., Pakdel, F., Segner, H. E., Kah, O., & Eggen, R. I. L. (2007) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2007.03.005 · Cited by 345
Corbin, C. J., Graham-Lorence, S. E., McPhaul, M. J., Mason, J. I., Mendelson, C. R., & Simpson, E. R. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.23.8948 · Cited by 298
Self-citationErickson, G. F., Garzo, V. G., & Magoffin, D. A. (1989) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-69-4-716 · Cited by 273
Metadata
- Authors
- Steinkampf, M. P., Mendelson, C. R., & Simpson, E. R.
- Affiliations
- The University of Texas System (US)
- University of Texas Health Science Center at Dallas (US)
- ORCID
- Mendelson, Carole R.
- Venue
- Molecular Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1987-07
- Volume
- 1
- Issue
- 7
- Pages
- 465-471
- ISSN
- 0888-8809 (ISSN-L)
- 1944-9917
- OpenAlex
- W2108477359
- Cited by
- 135
- References
- 27
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2022: 3 · 2021: 1 · 2020: 4 · 2019: 2 · 2018: 3 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 2 · 2013: 3 · 2012: 2
- FWCI
- 10.38
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Alabama at Birmingham (35294)
- National Institutes of Health (AM-31206)
- U.S. Public Health Service (5 T32)
- National Institute on Aging (AG-00306)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (T32HD007190, 5-T32 HD07190)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
- Keywords
- follicle-stimulating hormone, aromatase, human granulosa cells, enzyme synthesis, cAMP signaling, forskolin, NADPH-cytochrome P450 reductase, mRNA levels, steroidogenesis, oocyte retrieval
- MeSH terms
- Aromatase, Cells, Cultured, Female, Colforsin, Follicle Stimulating Hormone, Granulosa Cells, Humans, NADPH-Ferrihemoprotein Reductase, RNA, Messenger, Blotting, Northern
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
