The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Expression and Regulation of the Proenkephalin Gene in Rat Sertoli Cells
Open accessAbstract
Previous studies have shown that multiple forms of proenkephalin RNA are present in the rat and mouse testis that are cell type specific: a 1700-nucleotide transcript, unique to the testis, found in spermatogenic cells, and a 1450-nucleotide form of somatic cell origin. In the present studies, rat Sertoli cells are shown to contain the 1450-nucleotide form of proenkephalin RNA. The concentration of this transcript is quite low immediately after Sertoli cell isolation, but increases after 1 day in culture to a constant level. Proenkephalin RNA levels are stimulated severalfold by FSH and cAMP, but are not significantly affected by LH or testosterone. These studies demonstrate that Sertoli cells are at least one source of the somatic, 1450-nucleotide form of proenkephalin RNA in the rat testis. The expression of opioid peptide genes by Sertoli cells as well as by germ and Leydig cells is further evidence for the postulated autocrine or paracrine functions of these peptides in the testis.
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“In any case, the long-term persistence of (re-)activatable autoantigen-specific ThEx cells in patients in remission may well explain the less-than-perfect treatment efficacy of steroid-sparing agents, 64-67 depletion of CD19/CD20 memory B cells, 68,69 as well as exacerbation of disease by b -IFN. 27,70”
From Autoantigen-specific CD4+ T cells acquire an exhausted phenotype and persist in human antigen-specific autoimmune diseases (2024) · cites this paper as [64]
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Cites
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J., & Rutter, W. J. (1979) · Biochemistry · doi:10.1021/bi00591a005 · Cited by 21,725
Spermatogenic cells of the prepuberal mouse: isolation and morphological characterization
Bellve, A., Cavicchia, J. C., Millette, C. F., O'Brien, D. A., Bhatnagar, Y. M., & Dym, M. (1977) · The Journal of Cell Biology · doi:10.1083/jcb.74.1.68 · Cited by 1,386
Regulation of the Seminiferous Epithelium*
PARVINEN, M. (1982) · Endocrine Reviews · doi:10.1210/edrv-3-4-404 · Cited by 631
Pure Sertoli Cell Cultures: A New Model for the Study of Somatic—Germ Cell Interactions
Galdieri, M., Elio Ziparo, A., Palombi, F., Russo, M. A., & Stefanini, M. (1981) · Journal of Andrology · doi:10.1002/j.1939-4640.1981.tb00625.x · Cited by 409
Establishment and Characterization of Two Distinct Mouse Testicular Epithelial Cell Line
Matfier, J. P. (1980) · Biology of Reproduction · doi:10.1095/biolreprod23.1.243 · Cited by 396
Biochemistry of the enkephalins and enkephalin-containing peptides
Udenfriend, S., & Kilpatrick, D. L. (1983) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(83)90149-2 · Cited by 271
Self-citationSkinner, M. K., & Fritz, I. B. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.1.114 · Cited by 267
REGULATION OF THE TESTIS SERTOLI CELL BY FOLLICLE STIMULATING HORMONE
Means, A. R., Dedman, J. R., Tash, J. S., Tindall, D. J., SICKLE, M. V., & Welsh, M. J. (1980) · Annual Review of Physiology · doi:10.1146/annurev.ph.42.030180.000423 · Cited by 231
Howells, R., Kilpatrick, D. L., Bhatt, R. R., Monahan, J. J., Poonian, M. S., & Udenfriend, S. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.23.7651 · Cited by 188
Self-citationCharacterization and Localization of Proopiomelanocortin Messenger RNA in the Adult Rat Testis
Pintar, J. E., Schachter, B. S., Herman, A. B., Durgerian, S., & Krieger, D. T. (1984) · Science · doi:10.1126/science.6740329 · Cited by 183
Research works printed but not linked: 1
- Kierzenbaum AL, Tres LL 1981 Structure and function of Sertoli cells in culture. In: Jagiello G, Vogel HJ (eds) Bioregulators of Reproduction. Academic Press, NY, pp 207-228
Cited by
Gnessi, L., Fabbri, A., & Spera, G. (1997) · Endocrine Reviews · doi:10.1210/edrv.18.4.0310 · Cited by 306
“cells isolated from 20-day-old rats (281, 282).”
BackgroundKilpatrick, D. L., Zinn, S. A., Fitzgerald, M., Higuchi, H., Sabol, S. L., & Meyerhardt, J. A. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.7.3717 · Cited by 108
Self-citationSaggau, C., Bacher, P., Esser, D., Rasa, M., Meise, S., Mohr, N., Kohlstedt, N., Hutloff, A., Schacht, S.-S., Dargvainiene, J., et al. (2024) · Immunity · doi:10.1016/j.immuni.2024.08.005 · Cited by 79
Stage-Specific Regulation of Stem Cell Factor Gene Expression in the Rat Seminiferous Epithelium
Yan, W., Linderborg, J., Suominen, J., & Toppari, J. (1999) · Endocrinology · doi:10.1210/en.140.3.1499 · Cited by 74
Regulation of Male Fertility by the Opioid System
Subirán, N., Casis, L., & Irazusta, J. (2011) · Molecular Medicine · doi:10.2119/molmed.2010.00268 · Cited by 67
Proenkephalin products are stored in the sperm acrosome and may function in fertilization.
Kew, D., Muffly, K. E., & Kilpatrick, D. L. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.23.9143 · Cited by 52
Self-citation4 Local control systems within the testis
Verhoeven, G. (1992) · Baillière s Clinical Endocrinology and Metabolism · doi:10.1016/s0950-351x(05)80152-1 · Cited by 48
Taylor, W. E., Najmabadi, H., Strathearn, M. D., Jou, N. T., Liebling, M., Rajavashisth, T. B., Chanani, N., Phung, L., & Bhasin, S. (1996) · Endocrinology · doi:10.1210/endo.137.12.8940364 · Cited by 51
Wang, H., Liu, F., Millette, C. F., & Kilpatrick, D. L. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.24.8478-8490.2002 · Cited by 37
“Membranes were hybridized with DNA probes (2 (cid:7) 10 6 cpm/ml) as described previously (21).”
Self-citationThe Effects of Testicular Denervation on Spermatogenesis in the Sprague- Dawley Rat
Chow, S.-H., Giglio, W., Anesetti, R., Ottenweller, J. E., POGACH, L. M., & Huang, H. F. S. (2000) · Neuroendocrinology · doi:10.1159/000054569 · Cited by 35
“as well as opioid-related proenkephalin gene in Sertoli cells [”
BackgroundRao, S. M., & Howells, R. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)80662-0 · Cited by 34
Collard, M. W., Day, R. W., Akil, H., Uhler, M. D., & DOUGLASS, J. O. (1990) · Molecular Endocrinology · doi:10.1210/mend-4-10-1488 · Cited by 34
“Although spermatogenic cells are the primary site of POMC and proenkephalin gene expression in testis, cultured Leydig and Sertoli cells show induced levels of proenkephalin mRNA upon treatment with CG and FSH, respectively (13,14).”
Background‘All that glisters is not gold’—common testis gene transcripts are not always what they seem
Ivell, R. (1992) · International Journal of Andrology · doi:10.1111/j.1365-2605.1992.tb01117.x · Cited by 34
A germ cell factor(s) modulates preproenkephalin gene expression in rat Sertoli cells
Fujisawa, M., Bardin, C. W., & Morris, P. L. (1992) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(92)90074-g · Cited by 31
Borsook, D., & Hyman, S. E. (1995) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1995.269.3.e393 · Cited by 29
Rosen, H., Itin, A., Schiff, R., & Keshet, E. (1990) · Molecular Endocrinology · doi:10.1210/mend-4-1-146 · Cited by 28
Garrett, J. E., & DOUGLASS, J. O. (1989) · Molecular Endocrinology · doi:10.1210/mend-3-12-2093 · Cited by 28
“Similar preliminary results for proenkephalin-derived peptides in cultured Sertoli cells have been mentioned (30).”
BackgroundResultDonovan, D. M., Takemura, M., O’Hara, B. F., Brannock, M. T., & Uhl, G. R. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.6.2345 · Cited by 27
Impact of morphine dependency and detoxification by methadone on male's rat reproductive system.
Ghowsi, M., & Yousofvand, N. (2015) · PubMed · Cited by 25
Wang, H., Sartini, B. L., Millette, C. F., & Kilpatrick, D. L. (2006) · Biology of Reproduction · doi:10.1095/biolreprod.106.052209 · Cited by 23
“Membranes were hybridized with a probe (2 3 10 cpm/ml), washed, and subjected to autoradiography as described previously [16].”
MethodSelf-citationProenkephalin transgenic mice: A short promoter confers high testis expression and reduced fertility
O’Hara, B. F., Donovan, D. M., Lindberg, I., Brannock, M. T., Ricker, D. D., Moffatt, C. A., Klaunberg, B. A., Schindler, C. W., CHANG, T. S. K., Nelson, R. J., et al. (1994) · Molecular Reproduction and Development · doi:10.1002/mrd.1080380308 · Cited by 23
“High levels of proenkephalin expression are found in somatic and germ cells, including Sertoli and peritubular cells in immature testis and Leydig and spermatogenic cells in adult testis (Kilpatrick and Millette, 1986; Kilpatrick and Rosenthal, 1986; Kilpatrick et al., 1987; Yoshikawa and Aizawa, 1988; Kew and Kilpatr…”
BackgroundLucas, T. F. G., Avellar, M. C. W., & Porto, C. S. (2004) · Life Sciences · doi:10.1016/j.lfs.2004.05.006 · Cited by 20
Interleukin-6 Regulation of Kappa Opioid Receptor Gene Expression in Primary Sertoli Cells
Jenab, S., & Morris, P. L. (2000) · Endocrine · doi:10.1385/endo:13:1:11 · Cited by 20
The role and regulation of testicular opioids
Fabbri, A. (1990) · Trends in Endocrinology and Metabolism · doi:10.1016/1043-2760(90)90020-4 · Cited by 16
Biosynthesis of Enkephalins and Proenkephalin-Derived Peptides
Rossier, J. (1993) · Handbook of experimental pharmacology · doi:10.1007/978-3-642-77460-7_18 · Cited by 17
Opioid Peptide Genes: Structure and Regulation
Höllt, V. (1991) · Springer eBooks · doi:10.1007/978-3-642-46660-1_2 · Cited by 12
Mehta, N. D., Don, J., Zinn, S. A., Millette, C. F., Wolgemuth, D. J., & Kilpatrick, D. L. (1994) · Endocrinology · doi:10.1210/en.135.4.1543 · Cited by 10
Self-citationKönig, W. (2000) · Ullmann's Encyclopedia of Industrial Chemistry · doi:10.1002/14356007.a19_061 · Cited by 11
Denef, C. (1998) · Comprehensive physiology · doi:10.1002/cphy.cp070118 · Cited by 5
Germ Cell Factor(S) Regulates Opioid Gene Expression in Sertoli Cells
Fujisawa, M., Bardin, C. W., & Morris, P. L. (1993) · Elsevier eBooks · doi:10.1016/b978-0-12-571148-7.50025-7 · Cited by 4
Opioid Peptide Genes: Structure and Regulation
Höllt, V. (1993) · Current topics in neuroendocrinology · doi:10.1007/978-3-642-77503-1_4 · Cited by 2
Alcohol, Opioids, and Testicular Function
Adams, M. L., & Cicero, T. J. (1995) · Humana Press eBooks · doi:10.1007/978-1-4612-0243-1_15 · Cited by 1
Opioid Peptide Expression in Peripheral Tissues and Its Functional Implications
Kilpatrick, D. L. (1993) · Handbook of experimental pharmacology · doi:10.1007/978-3-642-77540-6_22
Self-citationParacrine Control of Testicular Function: Role of Opioids
Bartlett, J. M. S. (1990) · Springer eBooks · doi:10.1007/978-3-642-75797-6_5
Opioidergic Regulation of Luteinizing Hormone Secretion and Gonadal Steroidogenesis in Male Rat
Saxena, R., & Kant, P. A. (1999) · Springer eBooks · doi:10.1007/978-1-4615-4805-8_9
Denef, C. (1997) · Comprehensive physiology · doi:10.1002/j.2040-4603.1997.tb01442.x
Metadata
- Authors
- Kew, D., & Kilpatrick, D. L.
- Venue
- Molecular Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1989-01
- Volume
- 3
- Issue
- 1
- Pages
- 179-184
- ISSN
- 0888-8809 (ISSN-L)
- 1944-9917
- OpenAlex
- W2048538190
- Cited by
- 36
- References
- 38
- Citations by year
- 2024: 1 · 2015: 1
- FWCI
- 5.34
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Andrew W. Mellon Foundation
- National Institutes of Health
- National Institute of Diabetes and Digestive and Kidney Diseases (DK-36468)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Keywords
- proenkephalin, Sertoli cells, testis, opioid peptides, gene expression regulation, follicle-stimulating hormone, cyclic AMP, luteinizing hormone, testosterone, autocrine and paracrine signaling
- MeSH terms
- Animals, Cells, Cultured, Enkephalins, Colforsin, Follicle Stimulating Hormone, Gene Expression Regulation, Male, Protein Precursors, RNA, Sertoli Cells, Dihydrotestosterone, Testosterone, Time Factors, 1-Methyl-3-isobutylxanthine, 8-Bromo Cyclic Adenosine Monophosphate, Rats
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