Identification of brain isoforms of the rat calcitonin receptor.
Abstract
Two rat calcitonin receptor isoforms have been identified by cDNA cloning from a hypothalamic library. The clones, C1a and C1b, specified proteins of 478 and 515 amino acids, respectively. The clones were identical, except that the C1b sequence encoded a 37-amino acid insert in the second extracellular domain, which conferred altered ligand recognition. Compared to the C1a receptor, expressed C1b receptors exhibited decreased affinity for porcine CT, relative to salmon CT, and negligible affinity for human CT. Clone C1b mRNA was predominately expressed in the brain, whereas mRNA for the C1a clone was present in both brain and peripheral tissues. Both receptors were able to couple functionally to adenylate cyclase. Thus, clone C1b represents a novel brain isoform of the CT receptor with different affinity for CT analogs resulting from an altered second extracellular domain.
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First CTR cDNA was cloned from porcine, followed by human and rat receptors.
“The first CTR cDNA was cloned from porcine (134), followed by the cloning of the receptors from human and rat (7,81,127,187).”
From The Activity of Peptides of the Calcitonin Family in Bone (2019) · cites this paper as [187]
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Self-citationForty years of calcitonin—where are we now? A tribute to the work of Iain Macintyre, FRS
Zaidi, M., Inzerillo, A. M., Moonga, B. S., Bevis, P. J. R., & Huang, C. L.-H. (2002) · Bone · doi:10.1016/s8756-3282(02)00688-9 · Cited by 151 · see citing works
The Activity of Peptides of the Calcitonin Family in Bone
Naot, D., Musson, D. S., & Cornish, J. (2019) · Physiological Reviews · doi:10.1152/physrev.00066.2017 · Cited by 141 · see citing works
Gorn, A. H., Rudolph, S. M., Flannery, M. R., Morton, C. C., Weremowicz, S., Wang, T. Z., Krane, S. M., & Goldring, S. R. (1995) · Journal of Clinical Investigation · doi:10.1172/jci117970 · Cited by 100 · see citing works
Calcitonin: Physiology or fantasy?
Davey, R. A., & Findlay, D. M. (2013) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.1869 · Cited by 101 · see citing works
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Hilton, J., Dowton, M., Houssami, S., & Sexton, P. (2000) · Journal of Endocrinology · doi:10.1677/joe.0.1660213 · Cited by 70 · see citing works
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- Authors
- Sexton, P. M., Houssami, S., Hilton, J. M., O'Keeffe, L. M., Center, R. J., Gillespie, M. T., Darcy, P., & Findlay, D. M.
- Affiliations
- St Vincents Institute of Medical Research (AU)
- Venue
- Molecular Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1993-06
- Volume
- 7
- Issue
- 6
- Pages
- 815-821
- ISSN
- 0888-8809 (ISSN-L)
- 1944-9917
- OpenAlex
- W2043610823
- Cited by
- 134
- References
- 0
- Citations by year
- 2025: 1 · 2023: 2 · 2021: 1 · 2020: 2 · 2019: 3 · 2018: 2 · 2017: 1 · 2015: 2 · 2013: 3 · 2012: 1
- FWCI
- 3.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Health Sciences › Medicine › Nephrology › Parathyroid Disorders and Treatments
- Keywords
- calcitonin receptor, receptor isoforms, alternative splicing, ligand recognition, brain expression, hypothalamus, adenylate cyclase coupling, cDNA cloning, rats
- MeSH terms
- Cyclic AMP, Adenylyl Cyclases, Amino Acid Sequence, Animals, Base Sequence, Brain Chemistry, Calcitonin, Cell Line, Cloning, Molecular, DNA, Molecular Sequence Data, Receptors, Cell Surface, RNA, Messenger, Tissue Distribution, Gene Expression, Receptors, Calcitonin, Rats
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