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Cited by 1Microtubule and mitosis dynamicsFungal and yeast genetics researchDevelopmental Biology and Gene Regulation

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The fission yeast cytokinetic ring component Fic1 promotes septum formation

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Rossi, Anthony M., Bohnert, K. Adam, Gould, Kathleen L.·2023·Biology Open
DOI 10.1242/bio.059957PMC PMC10184318

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Abstract

In Schizosaccharomyces pombe, septum formation is coordinated with cytokinetic ring constriction but the mechanisms linking these events are unclear. In this study, we explored the role of the cytokinetic ring component Fic1, first identified by its interaction with the F-BAR protein Cdc15, in septum formation. We found that the fic1 phospho-ablating mutant, fic1-2A, is a gain-of-function allele that suppresses myo2-E1, the temperature-sensitive allele of the essential type-II myosin, myo2. This suppression is achieved by the promotion of septum formation and required Fic1's interaction with the F-BAR proteins Cdc15 and Imp2. Additionally, we found that Fic1 interacts with Cyk3 and that this interaction was likewise required for Fic1's role in septum formation. Fic1, Cdc15, Imp2, and Cyk3 are the orthologs of the Saccharomyces cerevisiae ingression progression complex, which stimulates the chitin synthase Chs2 to promote primary septum formation. However, our findings indicate that Fic1 promotes septum formation and cell abscission independently of the S. pombe Chs2 ortholog. Thus, while similar complexes exist in the two yeasts that each promote septation, they appear to have different downstream effectors.

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Research works printed but not linked: 59

  1. Bahler, 1998, Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe, Yeast, 14, 943, 10.1002/(SICI)1097-0061(199807)14:10<943::AID-YEA292>3.0.CO;2-Y · DOI
  2. Balasubramanian, 1998, Isolation and characterization of new fission yeast cytokinesis mutants, Genetics, 149, 1265, 10.1093/genetics/149.3.1265 · DOI
  3. Bohnert, 2012, Cytokinesis-based constraints on polarized cell growth in fission yeast, PLoS Genet., 8, e1003004, 10.1371/journal.pgen.1003004 · DOI
  4. Bohnert, 2009, A link between aurora kinase and Clp1/Cdc14 regulation uncovered by the identification of a fission yeast borealin-like protein, Mol. Biol. Cell, 20, 3646, 10.1091/mbc.e09-04-0289 · DOI
  5. Bohnert, 2020, Phosphoregulation of the cytokinetic protein Fic1 contributes to fission yeast growth polarity establishment, J. Cell Sci., 133, jcs244392, 10.1242/jcs.244392 · DOI
  6. Chang, 2000, Sid4p is required to localize components of the septation initiation pathway to the spindle pole body in fission yeast, Proc. Natl. Acad. Sci. USA, 97, 5249, 10.1073/pnas.97.10.5249 · DOI
  7. Cheffings, 2016, Actomyosin ring formation and tension generation in eukaryotic cytokinesis, Curr. Biol., 26, R719, 10.1016/j.cub.2016.06.071 · DOI
  8. Cortés, 2002, Localization of the (1,3)beta-D-glucan synthase catalytic subunit homologue Bgs1p/Cps1p from fission yeast suggests that it is involved in septation, polarized growth, mating, spore wall formation and spore germination, J. Cell Sci., 115, 4081, 10.1242/jcs.00085 · DOI
  9. Cortés, 2007, The (1,3)β-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation, Mol. Microbiol., 65, 201, 10.1111/j.1365-2958.2007.05784.x · DOI
  10. Cortés, 2012, Fission yeast Ags1 confers the essential septum strength needed for safe gradual cell abscission, J. Cell Biol., 198, 637, 10.1083/jcb.201202015 · DOI
  11. Cortés, 2015, Cooperation between Paxillin-like protein Pxl1 and Glucan synthase Bgs1 is essential for actomyosin ring stability and septum formation in fission yeast, PLoS Genet., 11, e1005358, 10.1371/journal.pgen.1005358 · DOI
  12. Curto, 2014, Membrane organization and cell fusion during mating in fission yeast requires multipass membrane protein Prm1, Genetics, 196, 1059, 10.1534/genetics.113.159558 · DOI
  13. Demeter, 1998, imp2, a new component of the actin ring in the fission yeast Schizosaccharomyces pombe, J. Cell Biol., 143, 415, 10.1083/jcb.143.2.415 · DOI
  14. Devrekanli, 2012, Inn1 and Cyk3 regulate chitin synthase during cytokinesis in budding yeasts, J. Cell Sci., 125, 5453, 10.1242/jcs.109157 · DOI
  15. Fankhauser, 1995, The S. pombe cdc15 gene is a key element in the reorganization of F-actin at mitosis, Cell, 82, 435, 10.1016/0092-8674(95)90432-8 · DOI
  16. Foltman, 2016, Ingression progression complexes control extracellular matrix remodelling during cytokinesis in budding yeast, PLoS Genet., 12, e1005864, 10.1371/journal.pgen.1005864 · DOI
  17. Gould, 1991, Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function, EMBO J., 10, 3297, 10.1002/j.1460-2075.1991.tb04894.x · DOI
  18. Hagan, 1995, The product of the spindle formation gene sad1+ associates with the fission yeast spindle pole body and is essential for viability, J. Cell Biol., 129, 1033, 10.1083/jcb.129.4.1033 · DOI
  19. Horisberger, 1978, Localization of α-galactomannan and of wheat germ agglutinin receptors in Schizosaccharomyces pombe, Arch. Microbiol., 119, 107, 10.1007/BF00964260 · DOI
  20. Hoya, 2017, Traffic through the trans-golgi network and the endosomal system requires collaboration between exomer and clathrin adaptors in fission yeast, Genetics, 205, 673, 10.1534/genetics.116.193458 · DOI
  21. Humbel, 2001, In situ localization of β-glucans in the cell wall of Schizosaccharomyces pombe, Yeast, 18, 433, 10.1002/yea.694 · DOI
  22. Jochova, 1991, F-actin contractile rings in protoplasts of the yeast Schizosaccharomyces, Cell Biol. Int. Rep., 15, 607, 10.1016/0309-1651(91)90007-6 · DOI
  23. Johnson, 1973, Cell division in yeasts: movement of organelles associated with cell plate growth of Schizosaccharomyces pombe, J. Bacteriol., 115, 358, 10.1128/jb.115.1.358-366.1973 · DOI
  24. Katayama, 1999, Fission yeast alpha-glucan synthase Mok1 requires the actin cytoskeleton to localize the sites of growth and plays an essential role in cell morphogenesis downstream of protein kinase C function, J. Cell Biol., 144, 1173, 10.1083/jcb.144.6.1173 · DOI
  25. Keeney, 1994, Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe, Genetics, 136, 849, 10.1093/genetics/136.3.849 · DOI
  26. Kitayama, 1997, Type II myosin heavy chain encoded by the myo2 gene composes the contractile ring during cytokinesis in Schizosaccharomyces pombe, J. Cell Biol., 137, 1309, 10.1083/jcb.137.6.1309 · DOI
  27. Liu, 1999, Drc1p/Cps1p, a 1,3-β-glucan synthase subunit, is essential for division septum assembly in Schizosaccharomyces pombe, Genetics, 153, 1193, 10.1093/genetics/153.3.1193 · DOI
  28. Mangione, 2019, Molecular form and function of the cytokinetic ring, J. Cell Sci., 132, jcs226928, 10.1242/jcs.226928 · DOI
  29. Martin-Garcia, 2006, The fission yeast Chs2 protein interacts with the type-II myosin Myo3p and is required for the integrity of the actomyosin ring, J. Cell Sci., 119, 2768, 10.1242/jcs.02998 · DOI
  30. Martin-Garcia, 2003, In Schizosaccharomyces pombe chs2p has no chitin synthase activity but is related to septum formation, FEBS Lett., 549, 176, 10.1016/S0014-5793(03)00812-3 · DOI
  31. Matsuo, 2004, Genetic analysis of chs1+ and chs2+ encoding chitin synthases from Schizosaccharomyces pombe, Biosci. Biotechnol. Biochem., 68, 1489, 10.1271/bbb.68.1489 · DOI
  32. Mirdita, 2022, ColabFold: making protein folding accessible to all, Nat. Methods, 19, 679, 10.1038/s41592-022-01488-1 · DOI · Paper page
  33. Moreno, 1991, Molecular genetic analysis of fission yeast Schizosaccharomyces pombe, Methods Enzymol., 194, 795, 10.1016/0076-6879(91)94059-L · DOI · Paper page
  34. Mulvihill, 2006, A critical role for the type V myosin, Myo52, in septum deposition and cell fission during cytokinesis in Schizosaccharomyces pombe, Cell Motil. Cytoskeleton, 63, 149, 10.1002/cm.20113 · DOI
  35. Munoz, 2013, Extracellular cell wall β(1,3)glucan is required to couple septation to actomyosin ring contraction, J. Cell Biol., 203, 265, 10.1083/jcb.201304132 · DOI
  36. Naqvi, 2000, Type II myosin regulatory light chain relieves auto-inhibition of myosin-heavy-chain function, Nat. Cell Biol., 2, 855, 10.1038/35041107 · DOI
  37. Nishihama, 2009, Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae, J. Cell Biol., 185, 995, 10.1083/jcb.200903125 · DOI
  38. Palani, 2017, Motor activity dependent and independent functions of myosin II contribute to actomyosin ring assembly and contraction in Schizosaccharomyces pombe, Curr. Biol., 27, 751, 10.1016/j.cub.2017.01.028 · DOI
  39. Palani, 2018, Steric hindrance in the upper 50 kDa domain of the motor Myo2p leads to cytokinesis defects in fission yeast, J. Cell Sci., 131, jcs205625, 10.1242/jcs.205625 · DOI
  40. Pohlmann, 2010, Asp1, a conserved 1/3 inositol polyphosphate kinase, regulates the dimorphic switch in Schizosaccharomyces pombe, Mol. Cell. Biol., 30, 4535, 10.1128/MCB.00472-10 · DOI
  41. Pollard, 2012, Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis, Mol. Biol. Cell, 23, 2433, 10.1091/mbc.e11-07-0656 · DOI
  42. Prevorovsky, 2009, High environmental iron concentrations stimulate adhesion and invasive growth of Schizosaccharomyces pombe, FEMS Microbiol. Lett., 293, 130, 10.1111/j.1574-6968.2009.01515.x · DOI
  43. Proctor, 2012, Contributions of turgor pressure, the contractile ring, and septum assembly to forces in cytokinesis in fission yeast, Curr. Biol., 22, 1601, 10.1016/j.cub.2012.06.042 · DOI
  44. Ramos, 2019, Two S. pombe septation phases differ in ingression rate, septum structure, and response to F-actin loss, J. Cell Biol., 218, 4171, 10.1083/jcb.201808163 · DOI
  45. Ren, 2015, The Cdc15 and Imp2 SH3 domains cooperatively scaffold a network of proteins that redundantly ensure efficient cell division in fission yeast, Mol. Biol. Cell, 26, 256, 10.1091/mbc.E14-10-1451 · DOI
  46. Roberts-Galbraith, 2009, The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring, J. Cell Biol., 184, 113, 10.1083/jcb.200806044 · DOI
  47. Roberts-Galbraith, 2010, Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site, Mol. Cell, 39, 86, 10.1016/j.molcel.2010.06.012 · DOI
  48. Roncero, 2016, 9 Chitin Synthesis and Fungal Cell Morphogenesis, 167
  49. Saksela, 2012, SH3 domain ligand binding: what's the consensus and where's the specificity?, FEBS Lett., 586, 2609, 10.1016/j.febslet.2012.04.042 · DOI
  50. Sanchez-Diaz, 2008, Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast, Nat. Cell Biol., 10, 395, 10.1038/ncb1701 · DOI
  51. Sburlati, 1986, Chitin synthetase 2, a presumptive participant in septum formation in Saccharomyces cerevisiae, J. Biol. Chem., 261, 15147, 10.1016/S0021-9258(18)66844-9 · DOI
  52. Schmidt, 2002, In budding yeast, contraction of the actomyosin ring and formation of the primary septum at cytokinesis depend on each other, J. Cell Sci., 115, 293, 10.1242/jcs.115.2.293 · DOI
  53. Schneider, 2012, NIH Image to ImageJ: 25 years of image analysis, Nat. Methods, 9, 671, 10.1038/nmeth.2089 · DOI · Paper page
  54. Shaner, 2013, A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum, Nat. Methods, 10, 407, 10.1038/nmeth.2413 · DOI
  55. Shaw, 1991, The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle, J. Cell Biol., 114, 111, 10.1083/jcb.114.1.111 · DOI
  56. Sietsma, 1990, The occurrence of glucosaminoglycan in the wall of Schizosaccharomyces pombe, J. Gen. Microbiol., 136, 2261, 10.1099/00221287-136-11-2261 · DOI
  57. Silverman, 1988, Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae, Proc. Natl. Acad. Sci. USA, 85, 4735, 10.1073/pnas.85.13.4735 · DOI
  58. Willet, 2015, The F-BAR Cdc15 promotes contractile ring formation through the direct recruitment of the formin Cdc12, J. Cell Biol., 208, 391, 10.1083/jcb.201411097 · DOI
  59. Wloka, 2012, Mechanisms of cytokinesis in budding yeast, Cytoskeleton, 69, 710, 10.1002/cm.21046 · DOI

Metadata

Authors
Rossi, A. M., Bohnert, K. A., & Gould, K. L.
Affiliations
Vanderbilt University (US)
Corresponding author
Gould, Kathleen L.
ORCID
Venue
Biology Open
Publisher
The Company of Biologists
Published
2023-05-09
Volume
12
Issue
5
ISSN
2046-6390 (ISSN-L)
DOI
10.1242/bio.059957
PMC
PMC10184318
OpenAlex
W4375955339
Cited by
1
References
1
Citations by year
2024: 1
FWCI
0.14
Type
journal article
Language
en
Open access
Licence
Locations
  • Biology Open (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • PubMed Central (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Institutes of Health (T32 GM008554)
  • National Institute of General Medical Sciences (R35 GM131799)
  • Vanderbilt University
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
FIC1, septum formation, cytokinetic ring, cytokinesis, Schizosaccharomyces pombe, Cdc15, IGF2BP2, F-BAR proteins, gain-of-function alleles

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