Global expression analysis of gene regulatory pathways during endocrine pancreatic development
Abstract
To define genetic pathways that regulate development of the endocrine pancreas, we generated transcriptional profiles of enriched cells isolated from four biologically significant stages of endocrine pancreas development:endoderm before pancreas specification, early pancreatic progenitor cells,endocrine progenitor cells and adult islets of Langerhans. These analyses implicate new signaling pathways in endocrine pancreas development, and identified sets of known and novel genes that are temporally regulated, as well as genes that spatially define developing endocrine cells from their neighbors. The differential expression of several genes from each time point was verified by RT-PCR and in situ hybridization. Moreover, we present preliminary functional evidence suggesting that one transcription factor encoding gene (Myt1), which was identified in our screen, is expressed in endocrine progenitors and may regulate α, β andδ cell development. In addition to identifying new genes that regulate endocrine cell fate, this global gene expression analysis has uncovered informative biological trends that occur during endocrine differentiation.
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Several groups have carried out screens for novel genes expressed in the endoderm of Xenopus and mouse embryos using microarray or cDNA hybridization.
“Several groups have carried out screens for novel genes expressed in the endoderm of Xenopus and mouse embryos using microarray or cDNA hybridization [25-29].”
From A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE) (2007) · cites this paper as [27]
Despite identification of several endoderm enriched genes, no novel DE specific genes were identified.
“Despite the identification of several endoderm enriched genes, no novel DE specific genes were identified.”
From A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE) (2007) · cites this paper as [27]
Previous studies have used cDNA cloning or microarray analysis to screen for novel endoderm genes.
“Previous studies focusing on screening for novel endoderm genes have used cDNA cloning or microarray analysis [25-29,40].”
From A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE) (2007) · cites this paper as [27]
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References 64 · Cited by 229
- Authors
- Gu, G., Wells, J. M., Dombkowski, D., Preffer, F., Aronow, B., & Melton, D. A.
- Affiliations
- Harvard University (US)
- Massachusetts General Hospital (US)
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2004-01-01
- Volume
- 131
- Issue
- 1
- Pages
- 165-179
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2156314428
- Cited by
- 229
- References
- 64
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 1 · 2023: 2 · 2022: 4 · 2021: 6 · 2020: 6 · 2019: 7 · 2018: 6 · 2017: 7 · 2016: 2 · 2015: 11 · 2014: 16 · 2013: 8 · 2012: 10
- FWCI
- 12.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (F32DK009832, R01DK065949)
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Keywords
- endocrine pancreas development, gene regulatory pathways, gene expression profiling, pancreatic progenitor cells, endocrine progenitor cells, pancreatic islets, endoderm, Myt1, transcription factors, alpha cells, pancreatic beta cells, delta cells, signaling pathways, temporal gene regulation
- MeSH terms
- Animals, Cell Differentiation, DNA-Binding Proteins, Female, Embryonic and Fetal Development, Islets of Langerhans, Luminescent Proteins, Mice, Inbred ICR, Mice, Transgenic, Pregnancy, Protein-Tyrosine Kinases, Transcription Factors, Transcription, Genetic, Protein Serine-Threonine Kinases, In Situ Hybridization, Gene Expression Regulation, Developmental, Reverse Transcriptase Polymerase Chain Reaction, Green Fluorescent Proteins, Mice
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