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Cited by 73Developmental Biology and Gene RegulationNeurogenesis and neuroplasticity mechanismsCraniofacial Disorders and Treatments

Cell delamination in the mesencephalic neural fold and its implication for the origin of ectomesenchyme

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Lee, Raymond Teck Ho, Nagai, Hiroki, Nakaya, Yukiko, Sheng, Guojun, Trainor, Paul A., Weston, James A., Thiery, Jean Paul·2013·Development
DOI 10.1242/dev.094680PMC PMC4074292
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Abstract

The neural crest is a transient structure unique to vertebrate embryos that gives rise to multiple lineages along the rostrocaudal axis. In cranial regions, neural crest cells are thought to differentiate into chondrocytes, osteocytes, pericytes and stromal cells, which are collectively termed ectomesenchyme derivatives, as well as pigment and neuronal derivatives. There is still no consensus as to whether the neural crest can be classified as a homogenous multipotent population of cells. This unresolved controversy has important implications for the formation of ectomesenchyme and for confirmation of whether the neural fold is compartmentalized into distinct domains, each with a different repertoire of derivatives. Here we report in mouse and chicken that cells in the neural fold delaminate over an extended period from different regions of the cranial neural fold to give rise to cells with distinct fates. Importantly, cells that give rise to ectomesenchyme undergo epithelial-mesenchymal transition from a lateral neural fold domain that does not express definitive neural markers, such as Sox1 and N-cadherin. Additionally, the inference that cells originating from the cranial neural ectoderm have a common origin and cell fate with trunk neural crest cells prompted us to revisit the issue of what defines the neural crest and the origin of the ectomesenchyme.

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  • Within the cell mass, there appeared to be two sub-types of CNCC-like cells expressing either the mesenchyme-associated marker PDGFRα or the neuro-glial-associated markers SOX10 and P75.

    “Within the cell mass, there appeared to be two sub-types of CNCC-like cells expressing either the mesenchyme-associated marker PDGFRα or the neuro-glial-associated markers SOX10 and P75 (Extended Data Fig. 2)15,16.”

    From Hair-bearing human skin generated entirely from pluripotent stem cells (2020) · cites this paper as [16]

    The Introduction (page unavailable) associates PDGFRα with a proposed ectomesenchymal population, and the Results section on neural-ectoderm derivatives identifies Sox10 as a glial marker. The source therefore supports the mesenchymal versus neuro-glial distinction, but our copy does not identify P75 as a neuro-glial marker. The observation within the citing paper’s cell mass is attributed to its own Extended Data Fig. 2, not established by this source. (LLM, conf=0.84)

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Authors
Lee, R. T. H., Nagai, H., Nakaya, Y., Sheng, G., Trainor, P. A., Weston, J. A., & Thiery, J. P.
Affiliations
  • Agency for Science, Technology and Research (SG)
  • RIKEN (JP)
  • Stowers Institute for Medical Research (US)
  • University of Oregon (US)
Corresponding author
Thiery, Jean Paul
ORCID
Venue
Development
Publisher
The Company of Biologists
Published
2013-12-15
Volume
140
Issue
24
Pages
4890-4902
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
DOI
10.1242/dev.094680
PMC
PMC4074292
OpenAlex
W2060471959
Cited by
73
References
76
Citations by year
2026: 3 · 2025: 3 · 2024: 9 · 2023: 5 · 2022: 6 · 2021: 3 · 2020: 7 · 2019: 4 · 2018: 7 · 2017: 9 · 2016: 8 · 2015: 7 · 2014: 2
FWCI
2.54
Type
journal article
Language
en
Open access
Licence
Locations
  • Development (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • National University of Singapore (submittedVersion)
Funders
  • National Institute of Dental and Craniofacial Research (R01DE016082)
  • Core Research for Evolutional Science and Technology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Craniofacial Disorders and Treatments
Keywords
neural crest, ectomesenchyme, cell delamination, midbrain, epithelial-mesenchymal transition, neural crest origin, cell fate specification, chondrocytes, osteocytes, stromal cells, SOX1, N-cadherin, mouse embryo, chick embryo
MeSH terms
Animals, Cell Differentiation, Chick Embryo, Ectoderm, Mesencephalon, Mesoderm, Neural Crest, Cadherins, Cell Lineage, Embryo Culture Techniques, Mice, Neural Plate, SOXB1 Transcription Factors, Epithelial-Mesenchymal Transition

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