The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Out with the old, in with the new: reassessing morpholino knockdowns in light of genome editing technology
Abstract
Morpholino oligomers have been used widely and for many years in the zebrafish community to transiently knock down the function of target genes. It has often been difficult, however, to reliably discriminate between specific and non-specific effects, and thus generally accepted guidelines to control for morpholino side effects do not exist. In light of recent methodologies to generate mutant lines in virtually any zebrafish gene, we discuss these different approaches with a specific focus on how the first description of a loss-of-function phenotype in zebrafish should be accomplished.
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Citations
Cites
Efficient genome editing in zebrafish using a CRISPR-Cas system
Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K. (2013) · Nature Biotechnology · doi:10.1038/nbt.2501 · Cited by 2,937
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
Fu, Y., Sander, J. D., Reyon, D., Cascio, V. M., & Joung, J. K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2808 · Cited by 1,993 · cited 2×
“Third, the published evidence, although currently limited, suggests that the side effects of these nucleases are often negligible (Hruscha et al., 2013), even though both TALE and Crispr-Cas nucleases can bind and cleave off-target loci (Reyon et al., 2012; Fu et al., 2014).”
BackgroundYang, H., Wang, H., Shivalila, C. S., Cheng, A. W., Shi, L., & Jaenisch, R. (2013) · Cell · doi:10.1016/j.cell.2013.08.022 · Cited by 1,596
Spatiotemporal resolution of the Ntla transcriptome in axial mesoderm development
Shestopalov, I. A., Pitt, C. L. W., & Chen, J. K. (2012) · Nature Chemical Biology · doi:10.1038/nchembio.772 · Cited by 42
Cited by
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
Guidelines for morpholino use in zebrafish
Stainier, D. Y. R., Raz, E., Lawson, N. D., Ekker, S. C., Burdine, R. D., Eisen, J. S., Ingham, P. W., Schulte-Merker, S., Yelon, D., Weinstein, B. M., et al. (2017) · PLOS Genetics · doi:10.1371/journal.pgen.1007000 · Cited by 336
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Metadata
- Authors
- Schulte-Merker, S., & Stainier, D. Y. R.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2014-08-15
- Volume
- 141
- Issue
- 16
- Pages
- 3103-3104
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2112853317
- Cited by
- 182
- References
- 4
- FWCI
- 13.20
- Type
- journal article
- Language
- en
- Open access
- closed
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- Schulte-Merker, S., & Stainier, D. Y. R. (2014). Out with the old, in with the new: reassessing morpholino knockdowns in light of genome editing technology. Development. https://doi.org/10.1242/dev.112003
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