CoChat
Sign Up
Cited by 39Developmental Biology and Gene RegulationSlime Mold and Myxomycetes ResearchPlanarian Biology and Electrostimulation

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

A Serum Response Factor homolog is required for spore differentiation in Dictyostelium

Escalante, Ricardo, Sastre, Leandro·1998·Development
DOI 10.1242/dev.125.19.3801
Open this paper in CoChat — free account

Abstract

A homolog of the Serum Response Factor (SRF) has been isolated from Dictyostelium discoideum and its function studied by analyzing the consequences of its gene disruption. The MADS-box region of Dictyostelium SRF (DdSRF) is highly conserved with those of the human, Drosophila and yeast homologs. srfA is a developmentally regulated gene expressed in prespore and spore cells. This gene plays an essential role in sporulation as its disruption leads to abnormal spore morphology and loss of viability. The mutant spores were round and cellulose deposition seemed to be partially affected. Initial prestalk and prespore cell differentiation did not seem to be compromised in the mutant since the expression of several cell-type-specific markers were found to be unaffected. However, the mRNA level of the spore marker spiA was greatly reduced. Activation of the cAMP-dependent protein kinase (PKA) by 8-Br-cAMP was not able to fully bypass the morphological defects of srfA− mutant spores, although this treatment induced spiA mRNA expression. Our results suggest that DdSRF is required for full maturation of spores and participates in the regulation of the expression of the spore-coat marker spiA and probably other maturation genes necessary for proper spore cell differentiation.

Overview

Integrity signals

Registry

Crossref · 10.1242/dev.125.19.3801

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • In cluster 1/5/7, transcription and protein synthesis categories were up-regulated; 13 transcription genes were up and 2 down; 24 protein synthesis genes were over- and 5 under-expressed.

    “Categories such as transcription and protein synthesis were up-regulated. For transcription, 13 genes were up- and only 2 down-regulated, namely the MADS-box transcription factor srfC [38] and the putative transcription factor wrky1. Similarly for protein synthesis, which includes also genes encoding ribosomal proteins, 24 genes were over- and only 5 under-expressed (see Additional File 1).”

    From Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium (2008) · cites this paper as [38]

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 52

  1. Adachi, 1994, Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme-mediated integration of the blasticidin S resistance marker, Biochem. Biophys. Res. Commun, 205, 1808, 10.1006/bbrc.1994.2880 · DOI
  2. Affolter, 1994, The Drosophila SRF homolog is expressed in a subset of tracheal cells and maps within a genomic region required for tracheal development, Development, 120, 743, 10.1242/dev.120.4.743 · DOI
  3. Anjard, 1992, Overexpression of DdPK2 protein kinase causes rapid development and affects the intracellular cAMP pathway of Dictyostelium discoideum, Development, 115, 785, 10.1242/dev.115.3.785 · DOI
  4. Anjard, 1997, SDF, a new spore differentiation factor secreted by Dictyostelium cells is phosphorylated by the cAMP dependent protein kinase, Differentiation, 62, 43, 10.1046/j.1432-0436.1997.6210043.x · DOI
  5. Anjard, 1998, Signal transduction pathways leading to spore differentiation in Dictyostelium discoideum, Dev. Biol, 193, 145, 10.1006/dbio.1997.8804 · DOI
  6. Ausubel, 1992, Current Protocols in Molecular Biology
  7. Bürki, 1991, Isolation of two genes encoding putative protein kinases regulated during Dictyostelium discoideum development, Gene, 102, 57, 10.1016/0378-1119(91)90538-M · DOI
  8. Cahill, 1996, Signalling pathways: jack of all cascades, Current Biology, 6, 16, 10.1016/S0960-9822(02)00410-4 · DOI
  9. Chang, 1998, Evidence that the RdeA protein is a component of a multistep phosphorelay modulating rate of development in Dictyostelium, EMBO J, 17, 2809, 10.1093/emboj/17.10.2809 · DOI
  10. Dalton, 1992, Characterization of SAP-1, a protein recruited by Serum Response Factor to the c-fos Serum Response Element, Cell, 68, 597, 10.1016/0092-8674(92)90194-H · DOI
  11. Devine, 1983, Spore coat proteins of Dictyostelium discoideum are packaged in prespore vesicles, Dev. Biol, 99, 437, 10.1016/0012-1606(83)90293-2 · DOI
  12. Dolan, 1991, Cell-type-specific transcription in yeast, Biochim. Biophys. Acta, 1088, 155, 10.1016/0167-4781(91)90051-M · DOI
  13. Dubois, 1987, Characterization of two genes, ARGRI and ARGRIII, required for specific regulation of arginine metabolism in yeast, Mol. Gen. Genet, 207, 142, 10.1007/BF00331501 · DOI
  14. Escalante, 1995, Whole-mount in situ hybridization of cell-type-specific mRNAs in Dictyostelium, Dev. Biol, 171, 262, 10.1006/dbio.1995.1278 · DOI
  15. Guillemin, 1996, The pruned gene encodes the Drosophila serum response factor and regulates cytoplasmic outgrowth during terminal branching of the tracheal system, Development, 122, 1353, 10.1242/dev.122.5.1353 · DOI
  16. Hohl, 1969, Ultrastructure of spore differentiation in Dictyostelium: The prespore vacuole, J. Ultrastruct. Res, 26, 442, 10.1016/S0022-5320(69)90050-1 · DOI
  17. Hopper, 1993, Activation of the prespore and spore cell pathway of Dictyostelium differentiation by cAMP-dependent protein kinase and evidence for its upstream regulation by ammonia, EMBO J, 12, 2459, 10.1002/j.1460-2075.1993.tb05900.x · DOI
  18. Hopper, 1995, Protein kinase A is a positive regulator of spore coat gene transcription in Dictyostelium, Differentiation, 58, 183, 10.1046/j.1432-0436.1995.5830183.x · DOI
  19. Johansen, 1995, Serum response factor: transcriptional regulation of genes induced by growth factors and differentiation, Biochim. Biophys. Acta, 1242, 1
  20. Kay, 1989, Evidence that elevated intracellular cyclic AMP triggers spore maturation in Dictyostelium, Development, 105, 753, 10.1242/dev.105.4.753 · DOI
  21. Kuspa, 1992, Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA, Proc. Natl. Acad. Sci. USA, 89, 8803, 10.1073/pnas.89.18.8803 · DOI
  22. Loomis, 1996, Genetic networks that regulate development in Dictyostelium cells, Microbiol. Rev, 60, 135, 10.1128/mr.60.1.135-150.1996 · DOI
  23. Loomis, 1995, Consensus phylogeny of Dictyostelium, Experientia, 51, 1110, 10.1007/BF01944728 · DOI
  24. Maeda, 1988, Dual effects of cAMP on the stability of prespore vesicles and 8-bromo-cAMP enhanced maturation of spore and stalk cells of Dictyostelium discoideum, Devel. Growth Differ, 30, 573, 10.1111/j.1440-169X.1988.00573.x · DOI
  25. Mann, 1991, A developmentally regulated, putative serine/threonine protein kinase is essential for development in Dictyostelium, Mech. Dev, 35, 89, 10.1016/0925-4773(91)90060-J · DOI
  26. Mann, 1994, Expression of cAMP-dependent protein kinase in prespore cells is sufficient to induce spore cell differentiation in Dictyostelium, Proc. Natl. Acad. Sci. USA, 91, 10561, 10.1073/pnas.91.22.10561 · DOI
  27. Mohun, 1991, Expression of genes encoding the transcription factor SRF during early development of Xenopus laevis: identification of a CArG box-binding activity as SRF, EMBO J, 10, 933, 10.1002/j.1460-2075.1991.tb08027.x · DOI
  28. Montagne, 1996, The Drosophila serum response factor gene is required for the formation of intervein tissue of wing and is allelic to blistered, Development, 122, 2589, 10.1242/dev.122.9.2589 · DOI
  29. Norman, 1988, Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element, Cell, 55, 989, 10.1016/0092-8674(88)90244-9 · DOI
  30. Nuckolls, 1996, The Dictyostelium dual-specificity kinase splA is essential for spore differentiation, Development, 122, 3295, 10.1242/dev.122.10.3295 · DOI
  31. Nurrish, 1995, DNA binding specificity determinants in MADS-box transcription factors, Moll. Cell. Biol, 15, 4076, 10.1128/MCB.15.8.4076 · DOI
  32. Passmore, 1988, Saccharomyces cerevisiae protein involved in plasmid maintenance is necesary for mating of MATα cells. J. Mol. Biol, 204, 593
  33. Pellegrini, 1995, Structure of serum response factor core bound to DNA, Nature, 376, 490, 10.1038/376490a0 · DOI
  34. Pollock, 1990, A sensitive method for the determination of protein-DNA binding specificities, Nucleic Acids Res, 18, 6197, 10.1093/nar/18.21.6197 · DOI
  35. Pollock, 1991, Human SRF-related proteins: DNA-binding properties and potential regulatory targets, Genes Dev, 5, 2327, 10.1101/gad.5.12a.2327 · DOI
  36. Richardson, 1992, Disruption of the sporulation-specific gene spiA in Dictyostelium discoideum leads to spore instability, Genes Dev, 6, 1058, 10.1101/gad.6.6.1058 · DOI
  37. Richardson, 1991, A prespore gene, Dd31, expressed during culmination of Dictyostelium discoideum, Dev. Biol, 144, 269, 10.1016/0012-1606(91)90421-X · DOI
  38. Sambrook, 1989, Molecular Cloning: a Laboratory Manual
  39. Schultz, 1995, Signal transduction and growth control in yeast, Curr. Opin. Genet. Dev, 5, 31, 10.1016/S0959-437X(95)90050-0 · DOI
  40. Shaulsky, 1993, Cell type regulation in response to expression of ricin-A in Dictyostelium, Dev. Biol, 160, 85, 10.1006/dbio.1993.1288 · DOI
  41. Shaulsky, 1996, Developmental signal transduction pathways uncovered by genetic suppresors, Proc. Natl. Acad. Sci. USA, 93, 15260, 10.1073/pnas.93.26.15260 · DOI
  42. Shaulsky, 1998, A cAMP phosphodiesterase controls PKA-dependent differentiation, Development, 125, 691, 10.1242/dev.125.4.691 · DOI
  43. Shaulsky, 1995, A multidrug resistance transporter serine protease gene is required for prestalk specialization in Dictyostelium, Genes Dev, 9, 1111, 10.1101/gad.9.9.1111 · DOI
  44. Shaw, 1989, Nucleotide sequences of Dictyostelium discoideum developmentally regulated cDNAs rich in (AAC) imply proteins that contain clusters of asparagine, glutamine, or threonine, Mol. Gen. Genet, 218, 453, 10.1007/BF00332409 · DOI
  45. Shore, 1995, The MADS-box family of transcription factors, Eur. J. Biochem, 229, 1, 10.1111/j.1432-1033.1995.tb20430.x · DOI
  46. Sussman, 1987, Cultivation and synchronous morphogenesis of Dictyostelium under controlled experimental conditions, Meth. Cell Biol, 28, 9, 10.1016/S0091-679X(08)61635-0 · DOI
  47. Theißen, 1996, Classification and phylogeny of the MADS-box multigene family suggest define roles of MADS-box gene subfamilies in the morphologicas evolution of eukariotes, J. Mol. Evol, 43, 484, 10.1007/BF02337521 · DOI
  48. Thomason, 1998, An intersection of the cAMP/PKA and two component signal transduction systems in Dictyostelium, 10.1093/emboj/17.10.2838 · DOI
  49. Treisman, 1992, The SRF and MCM1 transcription factors, Curr. Opin. Genet. Dev, 2, 221, 10.1016/S0959-437X(05)80277-1 · DOI
  50. Wang, 1996, A two-component histidine kinase gene that functions in Dictyostelium development, EMBO J, 15, 3890, 10.1002/j.1460-2075.1996.tb00763.x · DOI
  51. West, 1990, Formation of the Dictyostelium spore coat, Dev. Genet, 11, 492, 10.1002/dvg.1020110526 · DOI
  52. Wynne, 1992, SRF and MCM1 have related but distinct DNA binding specificities, Nucleic Acids Res, 20, 3297, 10.1093/nar/20.13.3297 · DOI

Cited by

Sorted by citations

Metadata

Authors
Escalante, R., & Sastre, L.
Affiliations
Consejo Superior de Investigaciones Científicas (ES)
Corresponding author
Escalante, Ricardo
ORCID
Venue
Development
Publisher
The Company of Biologists
Published
1998-10-01
Volume
125
Issue
19
Pages
3801-3808
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
DOI
10.1242/dev.125.19.3801
OpenAlex
W2150644367
Cited by
39
References
1
Citations by year
2026: 1 · 2025: 1 · 2021: 1 · 2018: 2 · 2016: 1 · 2015: 2 · 2013: 2
FWCI
0.94
Type
journal article
Language
en
Open access
closed
Licence
www.biologists.com/user-licence-1-1/ (vor)
Locations
  • Development (publishedVersion)
  • PubMed (publishedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Slime Mold and Myxomycetes Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Planarian Biology and Electrostimulation
Keywords
Dictyostelium discoideum, serum response factor, spore differentiation, sporulation, MADS-box transcription factors, spore coat, spore morphology, cellulose deposition, protein kinase A, prestalk cells, prespore cells, gene disruption
MeSH terms
Amino Acid Sequence, Animals, Cell Differentiation, Dictyostelium, DNA-Binding Proteins, Enzyme Activation, Humans, Molecular Sequence Data, Mutation, Nuclear Proteins, RNA, Messenger, Spores, 8-Bromo Cyclic Adenosine Monophosphate, Protozoan Proteins, RNA, Protozoan, Genes, Protozoan, Sequence Homology, Amino Acid, Cyclic AMP-Dependent Protein Kinases, Gene Expression Regulation, Developmental, Serum Response Factor

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review