Early mouse endoderm is patterned by soluble factors from adjacent germ layers
Abstract
Endoderm that forms the respiratory and digestive tracts is a sheet of approximately 500-1000 cells around the distal cup of an E7.5 mouse embryo. Within 2 days, endoderm folds into a primitive gut tube from which numerous organs will bud. To characterize the signals involved in the developmental specification of this early endoderm, we have employed an in vitro assay using germ layer explants and show that adjacent germ layers provide soluble, temporally specific signals that induce organ-specific gene expression in endoderm. Furthermore, we show that FGF4 expressed in primitive streak-mesoderm can induce the differentiation of endoderm in a concentration-dependent manner. We conclude that the differentiation of gastrulation-stage endoderm is directed by adjacent mesoderm and ectoderm, one of the earliest reported patterning events in formation of the vertebrate gut tube.s
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
At the 0-6 somite stage, the newly formed endoderm has not yet been patterned.
“At this stage the newly formed endoderm has not yet been patterned, based on endoderm explant experiments [14,34].”
From A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE) (2007) · cites this paper as [14]
FGF4, expressed in the neighboring cardiac mesoderm, can induce differentiation of ventral foregut endoderm in a concentration-dependent manner.
“FGF4, which is expressed in the neighboring cardiac mesoderm, can induce the differentiation of ventral foregut endoderm in a concentration-dependent manner [13,14].”
From A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE) (2007) · cites this paper as [14]
By the 8-12 somite stage, endoderm patterning has initiated.
“By this stage endoderm patterning has initiated [14,35].”
From A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE) (2007) · cites this paper as [14]
The citation lists haven’t been fetched yet
References 59 · Cited by 366
- Authors
- Wells, J. M., & Melton, D. A.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Wells, James M.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2000-04-15
- Volume
- 127
- Issue
- 8
- Pages
- 1563-1572
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2144107141
- Cited by
- 366
- References
- 59
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 1 · 2023: 2 · 2022: 5 · 2021: 12 · 2020: 7 · 2019: 8 · 2018: 12 · 2017: 15 · 2016: 12 · 2015: 15 · 2014: 17 · 2013: 14 · 2012: 15
- FWCI
- 6.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Development (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Keywords
- endoderm patterning, FGF4, organ-specific gene expression, gastrulation, endoderm differentiation, mouse embryo
- MeSH terms
- Animals, Chick Embryo, Digestive System, Ectoderm, Endoderm, Embryonic and Fetal Development, Fibroblast Growth Factors, Gastrula, Mesoderm, Mice, Inbred ICR, Notochord, Proto-Oncogene Proteins, Solubility, Signal Transduction, Gene Expression, Body Patterning, Mice, Fibroblast Growth Factor 4
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
