The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Longitudinal in vivo imaging of adult Danionella cerebrum using standard confocal microscopy
Abstract
Danionella cerebrum is a new vertebrate model that offers an exciting opportunity to visualize dynamic biological processes in intact adult animals. Key advantages of this model include its small size, life-long optical transparency, genetic amenability and short generation time. Establishing a reliable method for longitudinal in vivo imaging of adult D. cerebrum while maintaining viability will allow in-depth image-based studies of various processes involved in development, disease onset and progression, wound healing, and aging in an intact live animal. Here, a method for both prolonged and longitudinal confocal live imaging of adult D. cerebrum using custom-designed and 3D-printed imaging chambers is described. Two transgenic D. cerebrum lines were created to test the imaging system, i.e. Tg(mpeg1:dendra2) and Tg(kdrl:mCherry-caax). The first line was used to visualize macrophages and microglia, and the second for spatial registration. By using this approach, differences in immune cell morphology and behavior during homeostasis as well as in response to a stab wound or two-photon-induced brain injury were observed in intact adult fish over the course of several days.
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Cites
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Inflammaging: a new immune–metabolic viewpoint for age-related diseases
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Sterile inflammation: sensing and reacting to damage
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The Tol2kit: A multisite gateway‐based construction kit for Tol2 transposon transgenesis constructs
Kwan, K. M., Fujimoto, E., Grabher, C., Mangum, B. D., Hardy, M. E., Campbell, D. S., Parant, J. M., Yost, H. J., Kanki, J. P., & Chien, C. (2007) · Developmental Dynamics · doi:10.1002/dvdy.21343 · Cited by 1,774
Transparent Adult Zebrafish as a Tool for In Vivo Transplantation Analysis
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mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish
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Neuroinflammation after Intracerebral Hemorrhage and Potential Therapeutic Targets
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Research works printed but not linked: 1
- Westerfield, 1993, The Zebrafish Book: A Guide for The Laboratory Use of Zebrafish (Brachydanio rerio)
Cited by
Penalva-Tena, A., Bedke, J., Gaudin, A., Barrios, J. P., Bertram, E. P. L., & Douglass, A. D. (2018) · doi:10.1101/444026 · Cited by 41
Zebrafish relatives as models for functional comparative genetics and genomics
Podobnik, M., Lu, Y., Irion, U., & Currie, P. D. (2026) · Nature Reviews Genetics · doi:10.1038/s41576-026-01007-4 · Cited by 1
Metadata
- Authors
- Lam, P.-Y.
- Venue
- Disease Models & Mechanisms
- Publisher
- The Company of Biologists
- Published
- 2022-12-01
- Volume
- 15
- Issue
- 12
- ISSN
- 1754-8403
- 1754-8411
- Cited by
- 33
- References
- 52
- Language
- en
- Funders
- National Institute of Neurological Disorders and Stroke (R00NS112599)
- Medical College of Wisconsin
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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