Laminin α5 influences the architecture of the mouse small intestine mucosa
Open accessAbstract
The mammalian intestine displays two distinct patterns of mucosal organization. The small intestine contains mucosal epithelial invaginations (the crypts of Lieberkühn) that are continuous with evaginations (villi) into the lumen. The colon also contains crypts of Lieberkühn, but its epithelial surface is lined by flat surface cuffs. The epithelial cells of both organs communicate with the underlying mesenchyme through a basement membrane that is composed of a variety of extracellular matrix proteins, including members of the laminin family. The basement membranes of the small intestine and colon contain distinct laminin subtypes; notably, the villus basement membrane is rich in laminin α5. Here, we show that the diminution of laminin α5 in a mouse model led to a compensatory deposition of colonic laminins, which resulted in a transformation from a small intestinal to a colonic mucosal architecture. The alteration in mucosal architecture was associated with reduced levels of nuclear p27Kip1 – a cell-cycle regulator – and altered intestinal epithelial cell proliferation, migration and differentiation. Our results suggest that laminin α5 has a crucial role in establishing and maintaining the specific mucosal pattern of the mouse small intestine.
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Matrigel may not contain all of the necessary components for proper organoid formation.
“Furthermore, Matrigel may not contain all of the necessary components for proper organoid formation; gut organoids cultured in Matrigel lack the characteristic architecture of mammalian intestines, which could be due to a sub-optimal amount of laminin-511 and the absence of other cell types such as mesenchymal cells49,50.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [49]
Gut organoids cultured in Matrigel lack the characteristic architecture of mammalian intestines.
“Furthermore, Matrigel may not contain all of the necessary components for proper organoid formation; gut organoids cultured in Matrigel lack the characteristic architecture of mammalian intestines, which could be due to a sub-optimal amount of laminin-511 and the absence of other cell types such as mesenchymal cells49,50.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [49]
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References 74 · Cited by 74
- Authors
- Mahoney, Z. X., Stappenbeck, T. S., & Miner, J. H.
- Affiliations
- Washington University in St. Louis (US)
- Corresponding author
- Miner, Jeffrey H.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2008-08-01
- Volume
- 121
- Issue
- 15
- Pages
- 2493-2502
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- PMC
- PMC2587097
- OpenAlex
- W2046890380
- Cited by
- 74
- References
- 74
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 5 · 2023: 3 · 2022: 2 · 2021: 14 · 2020: 8 · 2019: 3 · 2018: 6 · 2017: 3 · 2016: 3 · 2015: 2 · 2014: 5 · 2013: 3 · 2012: 2
- FWCI
- 1.94
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Cell Science (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (1c06rr015502-01)
- National Heart, Lung, and Blood Institute (P01HL029594)
- National Institute on Deafness and Other Communication Disorders (P30DC004665)
- National Institute of General Medical Sciences (R01GM060432)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK064687, P30DK079333, P30DK052574)
- National Center for Research Resources (C06-RR-015502)
- Topics
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Digestive system and related health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Keywords
- laminin alpha5, small intestinal mucosa, intestinal crypts, villi, basement membrane, extracellular matrix, p27, cell cycle regulation, intestinal epithelial cell proliferation, epithelial cell migration, epithelial differentiation, intestinal morphology
- MeSH terms
- Animals, Cell Differentiation, Cell Movement, Epithelial Cells, Intestinal Mucosa, Intestine, Small, Laminin, Lutheran Blood-Group System, Membrane Glycoproteins, Morphogenesis, Down-Regulation, Cell Adhesion Molecules, Apoptosis, Mice, Knockout, Transgenes, Cell Proliferation, Cyclin-Dependent Kinase Inhibitor p27, Mice
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