The DOI matches its Crossref record; 49 of the 50 references it cites were found in a registry or an index.
Identification of the membrane-type matrix metalloproteinase MT1-MMP in osteoclasts
Abstract
The osteoclasts are the cells responsible for bone resorption. Matrix metalloproteinases (MMPs) appear crucial for this process. To identify possible MMP expression in osteoclasts, we amplified osteoclast cDNA fragments having homology with MMP genes, and used them as a probe to screen a rabbit osteoclast cDNA library. We obtained a cDNA of 1,972 bp encoding a polypeptide of 582 amino acids that showed more than 92% identity to human, mouse, and rat membrane-type 1 MMP (MT1-MMP), a cell surface proteinase believed to trigger cancer cell invasion. By northern blotting, MT1-MMP was found to be highly expressed in purified osteoclasts when compared with alveolar macrophages and bone stromal cells, as well as with various tissues. In situ hybridization on bone sections showed that MT1-MMP is expressed also in osteoclasts in vivo. Antibodies recognizing MT1-MMP reacted with specific plasma membrane areas corresponding to lamellipodia and podosomes involved, respectively, in migratory and attachment activities of the osteoclasts. These observations highlight how cells might bring MT1-MMP into contact with focal points of the extracellular matrix, and are compatible with a role of MT1-MMP in migratory and attachment activities of the osteoclast.
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“MMP-14 is highly expressed on osteoclast podosomes,149 and it digests interstitial collagen as well as other ECM molecules.150”
From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [149]
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Research works printed but not linked: 3
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- Helfrich, 1994, Isolation of osteoclasts and osteoclast membranes, 128
- Sato, 1994, A simple method for isolation of rabbit osteoclasts, Dentistry in Japan, 31, 3
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The 'ins' and 'outs' of podosomes and invadopodia: characteristics, formation and function
Murphy, D. A., & Courtneidge, S. A. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3141 · Cited by 1,092
Membrane-Type 1 Matrix Metalloproteinase Cleaves Cd44 and Promotes Cell Migration
Kajita, M., Itoh, Y., Chiba, T., Mori, H., Okada, A., Kinoh, H., & Seiki, M. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.153.5.893 · Cited by 746
The matrix corroded: podosomes and invadopodia in extracellular matrix degradation
Linder, S. (2007) · Trends in Cell Biology · doi:10.1016/j.tcb.2007.01.002 · Cited by 640
Podosomes: adhesion hot-spots of invasive cells
Linder, S., & Aepfelbacher, M. (2003) · Trends in Cell Biology · doi:10.1016/s0962-8924(03)00128-4 · Cited by 615
Foot and mouth: podosomes, invadopodia and circular dorsal ruffles
Buccione, R., Orth, J. D., & McNiven, M. A. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1436 · Cited by 594
Metadata
- Authors
- Sato, T., Ovejero, M. D. C., Hou, P., Heegaard, A.-M., Kumegawa, M., Tækker Foged, N., & Delaissé, J.-M.
- Affiliations
- Center for Clinical & Basic Research (DK)
- 1 Department of Basic Research, Center for Clinical & Basic Research, Ballerup Byvej 222, DK-2750 Ballerup, Denmark
- Meikai University (JP)
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1997-03-01
- Volume
- 110
- Issue
- 5
- Pages
- 589-596
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2118837944
- Cited by
- 214
- References
- 50
- Citations by year
- 2025: 3 · 2024: 1 · 2022: 2 · 2021: 3 · 2020: 2 · 2019: 6 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 5 · 2014: 8 · 2013: 10 · 2012: 4
- FWCI
- 4.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cell Science (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Bone and Dental Protein Studies
- Keywords
- MT1-MMP, membrane-type matrix metalloproteinases, osteoclasts, bone resorption, cDNA cloning, gene expression, lamellipodia, podosomes, cell migration, cell attachment, extracellular matrix
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Humans, Immunohistochemistry, Metalloendopeptidases, Molecular Sequence Data, Osteoclasts, Rabbits, Blotting, Northern, Sequence Homology, Amino Acid, In Situ Hybridization, DNA, Complementary, Matrix Metalloproteinases, Membrane-Associated, Matrix Metalloproteinase 14
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