Actin-based motor properties of native myosin VIIa
Abstract
Myosin VIIa has critical roles in the inner ear and the retina. To help understand how this protein functions, native myosin VIIa was tested for mechanoenzymatic properties. Myosin VIIa was immunoprecipitated from retinal tissue and found to be associated with calmodulin in a Ca(2+)-sensitive manner. Myosin VIIa Mg-ATPase activity was detected; in the absence of Ca(2+) (i.e. with bound calmodulin), it was stimulated by f-actin with a K(cat) of 4.3 s(-1) and with 7 microM actin required for half-maximal activity. In a sliding filament motility assay, myosin VIIa moved actin filaments with a velocity of 190 nm s(-1). These results demonstrate that myosin VIIa is a calmodulin-binding protein and a bona fide actin-based motor.
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Myosin-7a is a functional actin motor.
“Myosin-7a has been demonstrated to be a functional actin motor (Udovichenko et al., 2002)”
From Microtubule motors transport phagosomes in the RPE, and lack of KLC1 leads to AMD-like pathogenesis (2015) · cites this paper as Udovichenko et al., 2002
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References 37 · Cited by 82
- Authors
- Udovichenko, I. P., Gibbs, D., & Williams, D. S.
- Affiliations
- University of California San Diego (US)
- Corresponding author
- Gibbs, Daniel
- Williams, David S.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2002-01-15
- Volume
- 115
- Issue
- 2
- Pages
- 445-450
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2288966434
- Cited by
- 82
- References
- 37
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 4 · 2017: 5 · 2016: 2 · 2015: 4 · 2014: 5 · 2012: 1
- FWCI
- 4.14
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cell Science (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- National Eye Institute (R01EY007042, EY07042)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiomyopathy and Myosin Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- MYO7A, calmodulin binding, Mg2+-ATPase activity, F-actin, calcium sensitivity, inner ear
- MeSH terms
- Myosin VIIa, Actins, Animals, Binding Sites, Calmodulin, Calmodulin-Binding Proteins, Cattle, Dyneins, Myosins, Retina, Ca(2+) Mg(2+)-ATPase, Calcium Signaling, Molecular Motor Proteins, Amino Acid Motifs, Protein Transport
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