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Cited by 12Genetic and Kidney Cyst DiseasesCystic Fibrosis Research AdvancesConnexins and lens biology

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The Small Interactor of PKD2 protein promotes the assembly and ciliary entry of the Chlamydomonas PKD2–mastigoneme complexes

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Das, Poulomi, Mekonnen, Betlehem, Alkhofash, Rama, Ingle, Abha V., Workman, E. Blair, Feather, Alec, Zhang, Gui, Chasen, Nathan, Liu, Peiwei, Lechtreck, Karl F.·2024·Journal of Cell Science
DOI 10.1242/jcs.261497PMC PMC10312728

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Abstract

In Chlamydomonas, the channel polycystin 2 (PKD2) is primarily present in the distal region of cilia, where it is attached to the axoneme and mastigonemes, extracellular polymers of MST1. In a smaller proximal ciliary region that lacks mastigonemes, PKD2 is more mobile. We show that the PKD2 regions are established early during ciliogenesis and increase proportionally in length as cilia elongate. In chimeric zygotes, tagged PKD2 rapidly entered the proximal region of PKD2-deficient cilia, whereas the assembly of the distal region was hindered, suggesting that axonemal binding of PKD2 requires de novo assembly of cilia. We identified the protein Small Interactor of PKD2 (SIP), a PKD2-related, single-pass transmembrane protein, as part of the PKD2–mastigoneme complex. In sip mutants, stability and proteolytic processing of PKD2 in the cell body were reduced and PKD2–mastigoneme complexes were absent from the cilia. Like the pkd2 and mst1 mutants, sip mutant cells swam with reduced velocity. Cilia of the pkd2 mutant beat with an increased frequency but were less efficient in moving the cells, suggesting a structural role for the PKD2–SIP–mastigoneme complex in increasing the effective surface of Chlamydomonas cilia.

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Metadata

Authors
Das, P., Mekonnen, B., Alkhofash, R., Ingle, A. V., Workman, E. B., Feather, A., Zhang, G., Chasen, N., Liu, P., & Lechtreck, K. F.
Affiliations
University of Georgia (US)
Corresponding author
Lechtreck, Karl F.
ORCID
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
2024-01-01
Volume
137
Issue
1
ISSN
  • 0021-9533 (ISSN-L)
  • 1477-9137
DOI
10.1242/jcs.261497
PMC
PMC10312728
OpenAlex
W4389479150
Cited by
12
References
2
Citations by year
2026: 3 · 2025: 2 · 2024: 7
FWCI
1.99
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Cell Science (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed Central (submittedVersion, cc-by-nc-nd)
Funders
  • University of Georgia
  • National Institutes of Health (R01GM139856)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
Keywords
PKD2, mastigonemes, ciliogenesis, Chlamydomonas, axoneme, ciliary motility, ciliary beat frequency, transmembrane proteins, protein complex formation

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