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Cited by 29Cellular transport and secretionLipid Membrane Structure and BehaviorSlime Mold and Myxomycetes Research

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Freeze-fracture study of phagocytosis in Dictyostelium discoideum

Favard-Séréno, Colette, Ludosky, Marie-Aline, Ryter, Antoinette·1981·Journal of Cell Science
DOI 10.1242/jcs.51.1.63
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Abstract

The plasma membrane and its derivative, the phagosome membrane, were studied during and after ingestion of yeast or latex beads in Dictyostelium discoideum. Freeze-fracture electron microscopy, which provides information on the internal architecture of the membranes, and observation of thin sections of cells treated by cytochemical methods were used in parallel. For visualization of membrane sterols in the replicas, the cells were fixed in the presence of digitonin or the antibiotic filipin. No lateral phase separation occurred during yeast engulfment: the intramembranous particles (IMPs), phospholipids and sterols remained distributed at random in the forming phagosome membrane. In contrast architectural modifications of the membrane were observed upon phagosome internalization. Compared to the plasma membrane, the phagosome membrane displayed 2–3 times more IMPs, a shift in the IMP size distribution and a higher sterol content. These changes were completed soon after phagosome closure; they were not related either to the nature of the ingested particles (yeast, latex beads) or to the pH in the membrane environment. The membrane changes took place when the phagosomes began to fuse with pre-existing digestive or autophagic vacuoles and lysosomes. Some of the experimental evidence suggests that the restructuring of the membrane may be related to the presence of hydrolases.

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Research works printed but not linked: 44

  1. Allen, 1976, Freeze fracture evidence for intramembrane changes accompanying membrane recycling in Paramecium, Cytobiologie, 12, 254
  2. Batz, 1976, Structural transformation of the phagosomal membrane in Tetrahymena cells endocytosing latex beads, Arch. Microbiol, 109, 215, 10.1007/BF00446631 · DOI
  3. Bowers, 1976, Changes in plasma membrane structure after internalization in phagosomes, J. Cell Biol, 70, 326, 10.1083/jcb.70.2.326 · DOI
  4. Bowers, 1980, A morphological study of plasma and phagosome membranes during endocytosis in Acanthamoeba, J. Cell Biol, 84, 246, 10.1083/jcb.84.2.246 · DOI
  5. Charalampous, 1979, Levels and distributions of phospholipids and cholesterol in the plasma membrane of neuroblastoma cells, Biochim. biophys. Acta, 556, 38, 10.1016/0005-2736(79)90417-6 · DOI
  6. De Bruyn, 1978, Non random distribution of sialic acid over the cell surface of bristle-coated endocytic vesicles of the sinusoidal endothelium, J. Cell Biol, 78, 379, 10.1083/jcb.78.2.379 · DOI
  7. De Chastellier, 1977, Changes of the cell surface and of the digestive apparatus of Dictyostelium discoideum during the starvation period triggering aggregation, J. Cell Biol, 75, 218, 10.1083/jcb.75.1.218 · DOI
  8. De Kruijff, 1974, Polyene antibiotic-sterol interactions in membranes of Acholeplasma laidlawii cells and lecithin liposomes, Biochim. biophys. Acta, 339, 57, 10.1016/0005-2736(74)90332-0 · DOI
  9. Edelson, 1974, Effects of concanavalin A on mouse peritoneal macrophages, J. exp. Med, 140, 1364, 10.1084/jem.140.5.1364 · DOI
  10. Elias, 1978, Freeze-fracture identification of steroldigitonin complexes in cell and liposome membranes, J. Cell Biol, 78, 577, 10.1083/jcb.78.2.577 · DOI
  11. Favard-Sereno, 1979, Plasma membrane structural changes correlated with the acquisition of aggregation competence in Dictyostelium discoideum, Biologie cell, 35, 45
  12. Finkelstein, 1973, Polyene antiobiotics, 377
  13. Fisher, 1976, Analysis of membrane halves: cholesterol, Proc. natn. Acad. Sci. U.S.A, 73, 173, 10.1073/pnas.73.1.173 · DOI
  14. Friend, 1980, Freeze-fracture alterations in Guinea pig sperm membranes preceding gamete fusion, 153
  15. Gonzales-Noriega, 1980, Chloroquine inhibits lysosomal enzyme pinocytosis and enhances lysosomal enzyme secretion by impairing receptor recycling, J. Cell Biol, 85, 839, 10.1083/jcb.85.3.839 · DOI
  16. Hohl, 1978, Plasma membrane alterations as a result of heat activation in Dictyostelium spores, Arch. Microbiol, 116, 239, 10.1007/BF00417846 · DOI
  17. Kitajima, 1976, Freeze-fracture ultrastructural alterations induced by filipin, pimaricin, nystatin and amphotericin B in the plasma membranes of Epidermophyton, Saccharomyces and red blood cells. A proposal of models for polyeneergosterol complex-induced membrane lesions, Biochem. biophys. Acta, 455, 452, 10.1016/0005-2736(76)90317-5 · DOI
  18. Kitajima, 1977, Differentiation of food vacuolar membranes during endocytosis in Tetrahymena, J. Cell Biol, 75, 436, 10.1083/jcb.75.2.436 · DOI
  19. Linder, 1978, Membrane fusion and specialized membranes of the contractile vacuole apparatus, J. Cell Biol, 79, 231a
  20. Loyter, 1980, Induction of membrane fusion in human erythrocyte ghosts: involvement of spectrin in the fusion process, 11
  21. Malhotra, 1980, Organization, composition and biogenesis of animal cell membranes, 1
  22. Moor, 1963, Fine structure in frozen-etched yeast cells, J. Cell Biol, 17, 609, 10.1083/jcb.17.3.609 · DOI
  23. Moore, 1978, Phagocytosis of bacteria by polymorphonuclear leukocytes, J. Cell Biol, 76, 158, 10.1083/jcb.76.1.158 · DOI
  24. Nachman, 1971, Macrophage plasma membrane, J. exp. Med, 133, 807, 10.1084/jem.133.4.807 · DOI
  25. Ohkuma, 1978, Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents, Proc. natn. Acad. Sci. U.S.A, 75, 3327, 10.1073/pnas.75.7.3327 · DOI
  26. Papahadjopoulos, 1973, Role of cholesterol in membranes. Effects of phospholipid-protein interactions, membrane permeability and enzymatic activity, Biochim. biophys. Acta, 330, 8, 10.1016/0005-2736(73)90280-0 · DOI
  27. Parish, 1974, Alkaline phosphatase of Dictyostelium discoideum-. cell surface location and colchicine effects on internalization during phagocytosis, FEBS Lett, 48, 293, 10.1016/0014-5793(74)80489-8 · DOI
  28. Plattner, 1974, Intramembranous changes on cationophore-triggered exocytosis in Paramecium, Nature, Lond, 252, 722, 10.1038/252722a0 · DOI
  29. Pryzwansky, 1979, Early degranulation of human neutrophils: immunocytochemical studies of surface and intracellular phagocytic events, Cell, 15, 1025, 10.1016/0092-8674(79)90215-0 · DOI
  30. Ryter, 1977, Morphometric and cytochemical studies of Dictyostelium discoideum in vegetative phase. Digestive system and membrane turnover, J. Cell Biol, 75, 200, 10.1083/jcb.75.1.200 · DOI
  31. Ryter, 1980, Electron-microscope study of Dictyostelium discoideum plasma membrane and its modifications during and after phagocytosis, J. Cell Sci, 41, 75, 10.1242/jcs.41.1.75 · DOI
  32. Satir, 1973, Membrane fusion in a model system. Mucocyst secretion in Tetrahymena, J. Cell Biol, 56, 153, 10.1083/jcb.56.1.153 · DOI
  33. Segal, 1980, Kinetics of fusion of the cytoplasmic granules with phagocytic vacuoles in human polymorphonuclear leukocytes, J. Cell Biol, 85, 42, 10.1083/jcb.85.1.42 · DOI
  34. Singer, 1971, The molecular organization of biological membranes, 146, 10.1016/B978-0-12-598650-2.50009-0 · DOI
  35. Smolen, 1974, Remodeling of granulocyte membrane fatty acids during phagocytosis, J. clin. Invest, 53, 726, 10.1172/JCI107611 · DOI
  36. Thorne, 1980, Eosinophil interaction with antibody-coated, non-phagocytosable surfaces: changes in cell surface proteins, J. Cell Sci, 42, 367, 10.1242/jcs.42.1.367 · DOI
  37. Tietze, 1980, Chloroquine and ammonium ion inhibit receptor-mediated endocytosis of mannose-glycoconjugates by macrophages: apparent inhibition of receptor recycling, Biochem. biophys. Res. Commun, 93, 1, 10.1016/S0006-291X(80)80237-3 · DOI
  38. Tillack, 1973, A freeze-etch study of the effects of filipin on liposomes and human erythrocyte membranes, Biochim. biophys. Acta, 323, 43, 10.1016/0005-2736(73)90430-6 · DOI
  39. Ukena, 1972, Effect of colchicine and vinblastine on the topographical separation of membrane functions, J. exp. Med, 136, 1, 10.1084/jem.136.1.1 · DOI
  40. Van Vliet, 1976, Isolation and characterization of subcellular membranes of Entamoeba invadens, J. Cell Biol, 71, 357, 10.1083/jcb.71.2.357 · DOI
  41. Verkleij, 1973, Freeze-etch electron microscopy of erythrocytes, Achole-plasma laidlauii cells and liposomal membranes after the action of filipin and amphotericin B, Biochim. Biophys. Acta, 291, 577, 10.1016/0005-2736(73)90509-9 · DOI
  42. Vincenzi, 1980, The plasma membrane calcium pump: regulation by a soluble Ca2+ binding protein, Fed. Proc, 39, 2427
  43. Wibo, 1974, Protein degradation in cultured cells, J. Cell Biol, 63, 430, 10.1083/jcb.63.2.430 · DOI
  44. Zwaal, 1973, Localization of red cell membrane constituents, Biochim. biophys. Acta, 300, 159, 10.1016/0304-4157(73)90003-8 · DOI

Cited by

Metadata

Authors
Favard-Séréno, C., Ludosky, M.-A., & Ryter, A.
Affiliations
Centre National de la Recherche Scientifique (FR)
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
1981-10-01
Volume
51
Issue
1
Pages
63-84
ISSN
  • 0021-9533 (ISSN-L)
  • 1477-9137
DOI
10.1242/jcs.51.1.63
OpenAlex
W2416124942
Cited by
29
References
0
Citations by year
2020: 1
FWCI
1.53
Type
journal article
Language
en
Open access
closed
Licence
www.biologists.com/user-licence-1-1/ (vor)
Locations
  • Journal of Cell Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Slime Mold and Myxomycetes Research
Keywords
phagocytosis, Dictyostelium discoideum, plasma membrane, membrane sterols, intramembrane particles, membrane lipid distribution, phagosome-lysosome fusion, autophagosomes, hydrolytic enzymes, latex beads
MeSH terms
Cell Membrane, Chloroquine, Concanavalin A, Dictyostelium, Freeze Fracturing, Hydrogen-Ion Concentration, Microscopy, Electron, Microspheres, Phagocytosis, Saccharomyces cerevisiae, Sterols

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