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Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies
Abstract
The detergent-insoluble T. brucei cytoskeleton consists of several morphologically distinct regions and organelles, many of which are detectable only by electron microscopy. We have produced a set of monoclonal antibodies that define each structural component of this highly ordered cytoskeleton. The monoclonal antibodies were selected by cloning of hybridomas produced from mice injected with complex mixtures of proteins of either the cytoskeleton itself or salt extracts thereof. Four antibodies define particular tubulin isotypes and locate the microtubules of the axoneme and sub-pellicular array; two antibodies recognize the flagellum attachment zone; one recognizes the paraflagellar rod and another the basal bodies. Finally, one antibody defines a detergent-insoluble component of the nucleus. The antigens detected by each monoclonal antibody have been analysed by immunofluorescence microscopy, immunogold electron microscopy and Western blotting.
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
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Kinesin-related cut 7 protein associates with mitotic and meiotic spindles in fission yeast
Hagan, I., & Yanagida, M. (1992) · Nature · doi:10.1038/356074a0 · Cited by 256
Mutations in CFAP43 and CFAP44 cause male infertility and flagellum defects in Trypanosoma and human
Coutton, C., Vargas, A. S., Amiri-Yekta, A., Kherraf, Z.-E., Ben Mustapha, S. F., Le Tanno, P., Wambergue-Legrand, C., Karaouzène, T., Martinez, G., Crouzy, S., et al. (2018) · Nature Communications · doi:10.1038/s41467-017-02792-7 · Cited by 245
Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes
Kschonsak, M., Merkel, F., Bisht, S., Metz, J., Rybin, V., Hassler, M., & Haering, C. H. (2017) · Cell · doi:10.1016/j.cell.2017.09.008 · Cited by 152
Paluh, J. L., Nogales, E., Oakley, B. R., McDonald, K., Pidoux, A. L., & Cande, W. Z. (2000) · Molecular Biology of the Cell · doi:10.1091/mbc.11.4.1225 · Cited by 125
Metadata
- Authors
- Woods, A., Sherwin, T., Sasse, R., Macrae, T. H., Baines, A. J., & Gull, K.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1989-07-01
- Volume
- 93
- Issue
- 3
- Pages
- 491-500
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2236011578
- Cited by
- 660
- References
- 1
- FWCI
- 8.59
- Type
- journal article
- Language
- en
- Open access
- closed
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- Woods, A., Sherwin, T., Sasse, R., Macrae, T. H., Baines, A. J., & Gull, K. (1989). Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies. Journal of Cell Science. https://doi.org/10.1242/jcs.93.3.491
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
