Distribution among tissues and intracellular localization of cofilin, a 21kDa actin-binding protein.
Open accessAbstract
Cofilin, a 21kDa actin-binding protein, binds to F-actin in a 1:1 molar ratio of cofilin to actin molecule (Nishida, E., S. Maekawa, and H. Sakai, Biochemistry, 23, 5307-5313, 1984) and is capable of controlling actin polymerization and depolymerization in vitro in a pH-sensitive manner (Yonezawa, N., E. Nishida, and H. Sakai, J. Biol. Chem., 260, 14410-14412, 1985). In this study, immunoblot analysis using monospecific antibodies against cofilin showed that cofilin is ubiquitously distributed in a variety of bovine and rat organs and tissues. Cofilin is also present in various cultured cell lines. Indirect immunofluorescence staining of mouse fibroblastic cells and human epidermoid carcinoma cells indicated that cofilin is distributed nearly uniformly in the cytoplasm and is concentrated in ruffling membranes where F-actin is also concentrated as revealed by staining with rhodamine-phalloin. Stress fiber structures were not strongly stained with the anti-cofilin antibody, although stress fiber staining was sometimes observed near the cell periphery in mouse 3T3 cells. These results suggest that the bulk of cofilin may not be associated with F-actin bundles in vivo.
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Cofilin binds to F-actin with a Kd < 0.05 μM and results in disassembly of F-actin.
“One of these binding partners is cofilin, a 21 kDa eukaryotic protein, which binds to F-actin with a K d < 0.05 µM33 and results in disassembly of F-actin34, 35.”
From Multivalent cross-linking of actin filaments and microtubules through the microtubule-associated protein Tau (2017) · cites this paper as [34]
The Cofilin section (page not provided) describes cofilin as a 21 kDa protein that binds F-actin, supporting that part of the statement attributed to this source. It does not give the cited F-actin Kd of <0.05 µM; its reported dissociation constant of 0.1–0.2 µM is for binding to monomeric actin. The separate disassembly statement carries other citations. (LLM, conf=0.88)
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Research works printed but not linked: 3
- SCHULDINER, S. and E. ROSENGURT. Na+/H+ antiport in Swiss 3T3 cells : Mitogenic stimu- lation leads to cytoplasmic alkalinization. Proc. Natl. Acad. Sci. U.S.A. 79, 7778-7782, 1982
- TOWBIN, H., T. STAEHELIN and J. GORDON. Electrophoretic transfer of proteins from poly- acrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 76, 4350-4354, 1979
- WULF, E., A. DEBODEN, F.A. BAUTZ, H. FAULSTICH and WIELAND, TH. Fluorescent phallo- toxin, a tool for the visualization of cellular actin. Proc. Natl. Acad. Sci. U.S.A. 76, 4498-4502, 1979
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Cabrales Fontela, Y., Kadavath, H., Biernat, J., Riedel, D., Mandelkow, E., & Zweckstetter, M. (2017) · Nature Communications · doi:10.1038/s41467-017-02230-8 · Cited by 148 · see citing works
- Authors
- Yonezawa, N., Nishida, E., Koyasu, S., Maekawa, S., Ohta, Y., Yahara, I., & Sakai, H.
- Affiliations
- Department of Biophysics and Biochemistry, Faculty of Science, University
- Tokyo Metropolitan Institute of Medical Science (JP)
- Venue
- Cell Structure and Function
- Publisher
- Japan Society for Cell Biology
- Published
- 1987
- Volume
- 12
- Issue
- 5
- Pages
- 443-452
- ISSN
- 0386-7196 (ISSN-L)
- 1347-3700
- OpenAlex
- W2084605173
- Cited by
- 60
- References
- 0
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 1 · 2018: 1 · 2017: 1 · 2014: 2 · 2013: 1 · 2012: 1
- FWCI
- 1.14
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cell Structure and Function (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Science and Technology Agency
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- cofilin, actin-binding proteins, tissue distribution, subcellular localization, F-actin, actin polymerization, actin depolymerization, stress fibers, cytoplasm, cultured cell lines, fibroblastic cells, epidermoid carcinoma cells
- MeSH terms
- Actins, Animals, Antibodies, Antigen-Antibody Complex, Brain Chemistry, Cell Line, Fibroblasts, Fluorescent Antibody Technique, Humans, KB Cells, Microfilament Proteins, Molecular Weight, Nerve Tissue Proteins, Protein Binding, Swine, Actin Depolymerizing Factors, Rats
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