Altered levels of oxidation and phospholipase c isozyme expression in the brains of theanine-administered rats
Open accessAbstract
Previously, we revealed that theanine, a green tea component, induced phospholipase C (PLC)-beta1 and -gamma1, stress-responsible molecules, in primary cultured rat cerebral cortical neurons, suggesting its protective effect on oxidative stress in neurons. In this study, we investigated whether the same favorable effect occurs in vivo. On the oral administration of theanine (10 mmol (1.74 g)/kg, once a day) to rats via gastric intubation for 2 weeks, there was no change in the weight of the body or the cerebral cortex (Cx), cerebellum (Cb), or hippocampus (Hip) in the brain. On assessment of oxidation levels in the brain with thiobarbiturate reactive substances as a marker, the levels were found to be 20% lower in the Cx of theanine-treated rats than in that of control ones. The protein expression levels of PLC-beta1 and -gamma1 were significantly increased in the Cx on theanine administration and the same tendency was observed in the Cb, but not the Hip. In addition, the protein expression level of PLC-delta1, which plays an opposite role to the other two isozymes, was not affected in any brain regions on theanine administration. Overall, it was demonstrated that theanine is a safe compound and its repeated oral administration reduces oxidation levels in the brain, especially the Cx, by increasing PLC-beta1 and -gamma1 protein expression, suggesting its favorable effect on the brain in vivo.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In rats, repeated administration of L-THE (10 mmol/kg body weight/day) through gastric intubation for two weeks caused a decrease in peroxidation levels of lipids within the brain.
“In another study in rats, repeated administration of L-THE, 10 mmol/kg body weight/day through gastric intubation for two weeks, caused a decrease in the peroxidation levels of lipids within the brain, which could be a positive effect.”
From l-Theanine as a Functional Food Additive: Its Role in Disease Prevention and Health Promotion (2016) · cites this paper as [50]
Repeated administration of L-THE in rats was associated with increasing protein expression levels of the gene coding protein PLC-β1, which is important for intracellular signalling of the cortex in the brain.
“More importantly, it was shown that repeated administration was associated with increasing protein expression levels of the gene coding protein PLC-β1, which is important for intracellular signalling of the cortex in the brain [50].”
From l-Theanine as a Functional Food Additive: Its Role in Disease Prevention and Health Promotion (2016) · cites this paper as [50]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 26 · Cited by 21
Research works printed but not linked: 1
- Nagasawa K., Nagai K., Fujimoto S., "Pathophysiological and Bio- chemical Analyses of "Life-style Related or Intractable Diseases," - Target Validation for Drug Therapy-," ed. by Nishino T., Takeuchi K., Research Signpost, Kerala, India, 2006, pp. 13-27.
- Authors
- Nishida, K., Yasuda, E., Nagasawa, K., & Fujimoto, S.
- Affiliations
- Kyoto Pharmaceutical University (JP)
- Venue
- Biological and Pharmaceutical Bulletin
- Publisher
- Pharmaceutical Society of Japan
- Published
- 2008
- Volume
- 31
- Issue
- 5
- Pages
- 857-860
- ISSN
- 0918-6158 (ISSN-L)
- 1347-5215
- OpenAlex
- W2071128892
- Cited by
- 21
- References
- 26
- Citations by year
- 2026: 1 · 2024: 1 · 2022: 2 · 2019: 2 · 2018: 1 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 1
- FWCI
- 1.18
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biological and Pharmaceutical Bulletin (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Tea Polyphenols and Effects
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- theanine, phospholipase C, PLCB1, PLCγ1, oxidative stress, cerebral cortex, cerebellum, hippocampus, neuroprotection
- MeSH terms
- Animals, Body Weight, Brain, Brain Chemistry, Catalase, Glutamates, Isoenzymes, Male, Organ Size, Oxidation-Reduction, Type C Phospholipases, Blotting, Western, Rats, Wistar, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
