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A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye.
Open accessAbstract
A new tetrazolium compound, sodium salt of 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1, 3-benzene disulfonate (1a), which produces a highly water-soluble formazan dye, due to the presence of two sulfonate groups, was synthesized and its potential utility evaluated in assays of NADH and cell proliferation. The compound proved to have a similar sensitivity to 2, 3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) in the assay of NADH and was also useful as an indicator of cell viability, with less cytotoxicity than XTT, in the proliferation assay using P388 cell lines.
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Cited by
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Tan, A. S., & Berridge, M. V. (2000) · Journal of Immunological Methods · doi:10.1016/s0022-1759(00)00156-3 · Cited by 330
UKEDA, H., KAWANA, D., MAEDA, S., & SAWAMURA, M. (1999) · Bioscience, Biotechnology, and Biochemistry · doi:10.1271/bbb.63.485 · Cited by 263
Dewan, M. Z. (2006) · Blood · doi:10.1182/blood-2005-02-0735 · Cited by 75
Kuriyama, K., Shimizu, T., Horiguchi, T., Watabe, M., & Abe, Y. (2002) · Inflammation Research · doi:10.1007/pl00012416 · Cited by 30
Metadata
- Authors
- ISHIYAMA, M., SHIGA, M., SASAMOTO, K., MIZOGUCHI, M., & HE, P.-G.
- Venue
- Chemical and Pharmaceutical Bulletin
- Publisher
- Pharmaceutical Society of Japan
- Published
- 1993
- Volume
- 41
- Issue
- 6
- Pages
- 1118-1122
- ISSN
- 0009-2363 (ISSN-L)
- 1347-5223
- OpenAlex
- W1515525226
- Cited by
- 488
- References
- 0
- FWCI
- 8.80
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye. https://app.cochat.ai/papers/10.1248/cpb.41.1118
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- ISHIYAMA, M., SHIGA, M., SASAMOTO, K., MIZOGUCHI, M., & HE, P.-G. (1993). A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye. Chemical and Pharmaceutical Bulletin. https://doi.org/10.1248/cpb.41.1118
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