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Development of a Fluorescent Indicator for Nitric Oxide Based on the Fluorescein Chromophore.
Open accessAbstract
Endogenous nitric oxide (NO) appears to modulate many physiological and pathophysiological processes. In order to obtain direct evidence for NO functions in vivo, we have developed 4,5-diaminofluorescein (DAF-2) as a novel fluorescent indicator for NO. Green-fluorescent triazolofluorescein formed by the reaction of NO and DAF-2 affords high sensitivity for NO (detection limit: 5 nM). Membrane-permeable DAF-2 diacetate (DAF-2 DA) was loaded into activated rat aortic smooth muscle cells, where the ester bonds are hydrolyzed by intracellular esterase, generating DAF-2. The fluorescence in the cells increased in a NO concentration-dependent manner. This imaging method should be useful for studies of the dynamic biological actions of NO at the molecular level with fine temporal and spatial resolution.
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Cited by
Nitric oxide in plants: an assessment of the current state of knowledge
Mur, L. A. J., Mandon, J., Persijn, S., Cristescu, S. M., Moshkov, I. E., Novikova, G. V., Hall, M. A., Harren, F. J. M., Hebelstrup, K. H., & Gupta, K. J. (2013) · AoB Plants · doi:10.1093/aobpla/pls052 · Cited by 481
Nagano, T., & Yoshimura, T. (2002) · Chemical Reviews · doi:10.1021/cr010152s · Cited by 414
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Kawashima, K. (2000) · Pharmacology & Therapeutics · doi:10.1016/s0163-7258(99)00071-6 · Cited by 384
Goel, S., Gupta, N., Walcott, B. P., Snuderl, M., Kesler, C. T., Kirkpatrick, N. D., Heishi, T., Huang, Y., Martin, J. D., Ager, E., et al. (2013) · JNCI: Journal of the National Cancer Institute · doi:10.1093/jnci/djt164 · Cited by 117
Villalba, N., Sackheim, A. M., Nunez, I. A., Hill-Eubanks, D. C., Nelson, M. T., Wellman, G. C., & Freeman, K. (2017) · Journal of Neurotrauma · doi:10.1089/neu.2015.4340 · Cited by 99
Metadata
- Authors
- KOJIMA, H., SAKURAI, K., KIKUCHI, K., KAWAHARA, S., KIRINO, Y., NAGOSHI, H., HIRATA, Y., & NAGANO, T.
- Venue
- Chemical and Pharmaceutical Bulletin
- Publisher
- Pharmaceutical Society of Japan
- Published
- 1998
- Volume
- 46
- Issue
- 2
- Pages
- 373-375
- ISSN
- 0009-2363 (ISSN-L)
- 1347-5223
- OpenAlex
- W2052239024
- Cited by
- 293
- References
- 0
- FWCI
- 9.47
- Type
- journal article
- Language
- en
- Open access
- bronze
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- KOJIMA, H., SAKURAI, K., KIKUCHI, K., KAWAHARA, S., KIRINO, Y., NAGOSHI, H., HIRATA, Y., & NAGANO, T. (1998). Development of a Fluorescent Indicator for Nitric Oxide Based on the Fluorescein Chromophore. Chemical and Pharmaceutical Bulletin. https://doi.org/10.1248/cpb.46.373
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