Widespread use of poly(A) tail length control to accentuate expression of the yeast transcriptome
Open accessAbstract
Control of poly(A) tail length can affect translation and stability of eukaryotic mRNAs. Although well established for individual cases, it was not known to what extent this type of adjustable gene control is used to shape expression of eukaryotic transcriptomes. Here we report on microarray-based measurements of mRNA poly(A) tail lengths and association with the poly(A)-binding protein Pab1 in S. cerevisiae , revealing extensive correlation between tail length and other physical and functional mRNA characteristics. Gene ontology analyses and further directed experiments indicate coregulation of tail length on functionally and cytotopically related mRNAs to coordinate cell-cycle progression, ribosome biogenesis, and retrotransposon expression. We show that the 3′-untranslated region drives transcript-specific adenylation control and translational efficiency of multiple mRNAs. Our findings suggest a wide-spread interdependence between 3′-untranslated region-mediated poly(A) tail length control, Pab1 binding, and mRNA translation in budding yeast. They further provide a molecular explanation for deadenylase function in the cell cycle and suggest additional cellular processes that depend on control of mRNA polyadenylation.
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Ribosomal protein and other housekeeping genes are enriched among short-tailed genes in yeast.
“This enrichment was strong in yeast, despite previous reports that ribosomal-protein genes tend to have long tails10,11.”
From Poly(A)-tail profiling reveals an embryonic switch in translational control (2014) · cites this paper as [10]
The paper's yeast results differed from earlier reports, which the paper attributes to the limitations described above.
“Our results in yeasts differed from those reported earlier10,11, which we again attribute to the limitations described above.”
From Poly(A)-tail profiling reveals an embryonic switch in translational control (2014) · cites this paper as [10]
The cycloheximide effect may have distorted TE measurements in studies that used polysome gradient fractionation followed by microarray analysis, including those reporting a positive link between ribosome density and poly(A)-tail length.
“This cycloheximide effect might have distorted the TE measurements in studies that calculated ribosome densities using polysome gradient fractionation followed by microarray analysis, including those reporting a positive correlation between ribosome density and poly(A)-tail length10,11.”
From Poly(A)-tail profiling reveals an embryonic switch in translational control (2014) · cites this paper as [10]
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References 79 · Cited by 144
- Authors
- Beilharz, T. H., & Preiss, T.
- Affiliations
- Victor Chang Cardiac Research Institute (AU)
- Corresponding author
- Preiss, Thomas
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2007-06-22
- Volume
- 13
- Issue
- 7
- Pages
- 982-997
- ISSN
- 1355-8382 (ISSN-L)
- 1469-9001
- PMC
- PMC1894919
- OpenAlex
- W2169290305
- Cited by
- 144
- References
- 79
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 3 · 2023: 5 · 2022: 8 · 2021: 9 · 2020: 5 · 2019: 8 · 2018: 8 · 2017: 5 · 2016: 4 · 2015: 14 · 2014: 11 · 2013: 6 · 2012: 13
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- RNA (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- ANU Open Research (Australian National University) (submittedVersion)
- Funders
- University of New South Wales
- LG Display
- Medical Research Council
- Australian Research Council
- National Health and Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- poly(A) tail length, mRNA polyadenylation, 3' untranslated region, mRNA translation, mRNA stability, deadenylase, cell cycle, ribosome biogenesis, retrotransposon expression, Saccharomyces cerevisiae, transcriptome regulation
- MeSH terms
- Base Sequence, Cell Cycle, Models, Biological, Molecular Sequence Data, Poly A, Protein Binding, RNA, Messenger, Saccharomyces cerevisiae, Protein Biosynthesis, Gene Expression Regulation, Fungal, Genome, Fungal, Sequence Analysis, RNA, Polyadenylation, Saccharomyces cerevisiae Proteins, Poly(A)-Binding Proteins, Microarray Analysis
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