Therapeutic Antidepressant Potential of NONHSAG045500 in Regulating Serotonin Transporter in Major Depressive Disorder
Open accessAbstract
BACKGROUND Major depressive disorder (MDD) is a chronic, life-threatening, highly disabling disease. Standardized treatment with fewer adverse effects, quick onset, and long-term maintenance of the effects of brief treatment for MDD is always being pursued. Long non-coding RNAs (lncRNAs) are highly expressed in the central nervous system and are involved in the occurrence and development of neurodegenerative and psychiatric diseases. This study aimed to investigate whether the overexpression and interference of 3 differentially down-regulated lncRNAs (NONHSAT142707, NONHSAG045500, and ENST00000517573) in MDD can affect the expression of central neurotransmitter serotonin (5-hydroxytryptamine) transporter (SERT) in vitro. MATERIAL AND METHODS First, we synthesized and validated the effect of 3 lncRNA plasmids and small interfering RNAs (siRNAs); next, we transfected the plasmids and siRNAs that caused significant overexpression or interference in SK-N-SH cells, and tested the expression of SERT by qRT-PCR. RESULTS The results showed that 3 lncRNA plasmids and siRNAs2 caused overexpression and interference, respectively. Only the overexpression of NONHSAG045500 could significantly inhibit the expression of SERT; interference with NONHSAG045500 could significantly strengthen the expression of SERT. CONCLUSIONS This study indicated that the expression of SERT could be regulated by up-regulating or down-regulating NONHSAG045500 expression and suggested that NONHSAG045500 could potentially be established as a new therapeutic target of MDD. Future work may be needed to definitively determine the correlation between NONHSAG045500 and SERT in vivo.
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NONHSAG045500 may modify the transport of monoamine neurotransmitters.
“NONHSAG045500 may modify the transport of monoamine neurotransmitters.316”
From Epigenetic regulation in major depression and other stress-related disorders: molecular mechanisms, clinical relevance and therapeutic potential (2023) · cites this paper as [316]
The Discussion reports that changing NONHSAG045500 expression altered serotonin transporter (SERT) expression in SK-N-SH cells in vitro. The Conclusions suggest this might modulate serotonin transmission, but the paper does not establish effects on the transport of other monoamine neurotransmitters or directly measure neurotransmitter transport. (LLM, conf=0.86)
Experimental — accuracy not yet measuredUpregulated lncRNA NONHSAG045500 inhibits the expression of the 5-HT transporter SERT, influencing 5-HT transmission in depression.
“Researchers have shown that upregulated lncRNA NONHSAG045500 inhibits the expression of the 5-HT transporter SERT which influences 5-HT transmission in depression.316”
From Epigenetic regulation in major depression and other stress-related disorders: molecular mechanisms, clinical relevance and therapeutic potential (2023) · cites this paper as [316]
The Results report that experimentally overexpressing NONHSAG045500 significantly reduced SERT expression in SK-N-SH cells. The Conclusions describe an in-vitro role in serotonin transmission but say its effects in pathological conditions might occur; the paper does not establish that upregulation alters 5-HT transmission in people with depression. (LLM, conf=0.88)
Experimental — accuracy not yet measuredInterference with NONHSAG045500 levels increases 5-HT concentration.
“Meanwhile, the concentration of 5-HT was increased by interference with the levels of NONHSAG045500.”
From Epigenetic regulation in major depression and other stress-related disorders: molecular mechanisms, clinical relevance and therapeutic potential (2023) · cites this paper as [316]
The Results section reports that interfering with NONHSAG045500 increased SERT expression in SK-N-SH cells, not that it increased 5-HT concentration. A search for 5-HT concentration and serotonin levels found no measurement of either following interference. (LLM, conf=0.78)
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References 35 · Cited by 18
- Authors
- Liu, S., Zhou, B., Wang, L., Hu, H., Yao, C., Cai, Z., & Cui, X.
- Affiliations
- Changzhou Institute of Technology (CN)
- Nanjing Medical University (CN)
- Nanjing Maternity and Child Health Care Hospital (CN)
- Corresponding author
- Cui, Xuelian
- Venue
- Medical Science Monitor
- Published
- 2018-06-29
- Volume
- 24
- Pages
- 4465-4473
- ISSN
- 1234-1010 (ISSN-L)
- 1643-3750
- PMC
- PMC6055515
- OpenAlex
- W2810779194
- Cited by
- 18
- References
- 35
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 5 · 2019: 1
- FWCI
- 1.61
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Medical Science Monitor (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pharmacology › Treatment of Major Depression
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- major depressive disorder, long non-coding RNA, serotonin transporter, antidepressant targets, lncRNA regulation of gene expression, siRNA knockdown, lncRNA overexpression, SK-N-SH cells
- MeSH terms
- Antidepressive Agents, Cell Line, Depressive Disorder, Major, Major Depressive Disorder, Humans, Serotonin, RNA, Small Interfering, Serotonin Plasma Membrane Transport Proteins, RNA, Long Noncoding
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