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In Vitro Survey of α-Glucosidase Inhibitory Food Components
Abstract
A survey of food components with alpha-glucosidase (AGH) inhibitory activity was conducted to identify a prophylactic effect for diabetes in food. Sardine muscle hydrolyzed by alkaline protease showed potent activity (IC50 = 48.7 mg/ml) as well as green and oolong teas (IC50 = 11.1 and 11.3 mg/ml, respectively). Furthermore, hydrolyzates prepared by various proteases gave differing AGH inhibitory activity. DEAE-Sephadex chromatography of the alkaline protease hydrolyzate eluted potent AGH inhibitors (IC50 = 15.6 mg/ml) with a 50 mM phosphate buffer (pH 7.0) containing 0.3 M NaCl, and their subsequent separation by HPLC in an ODS column showed that there were some inhibitors possessing primary amino groups. This indicates that they would have been high anionic and peptidic compounds.
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Cited by
Vilcacundo, R., Martínez-Villaluenga, C., & Hernández-Ledesma, B. (2017) · Journal of Functional Foods · doi:10.1016/j.jff.2017.06.024 · Cited by 264
Schäfer, A., & Högger, P. (2007) · Diabetes Research and Clinical Practice · doi:10.1016/j.diabres.2006.10.011 · Cited by 136
Gheda, S., Naby, M. A., Mohamed, T., Pereira, L., & Khamis, A. (2021) · Environmental Science and Pollution Research · doi:10.1007/s11356-021-12347-5 · Cited by 85
Metadata
- Authors
- Matsui, T., Yoshimoto, C., Osajima, K., Oki, T., & Osajima, Y.
- Venue
- Bioscience, Biotechnology, and Biochemistry
- Publisher
- Informa UK Limited
- Published
- 1996-01
- Volume
- 60
- Issue
- 12
- Pages
- 2019-2022
- ISSN
- 0916-8451 (ISSN-L)
- 1347-6947
- OpenAlex
- W1999302387
- Cited by
- 229
- References
- 0
- FWCI
- 0.28
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: In Vitro Survey of α-Glucosidase Inhibitory Food Components. https://app.cochat.ai/papers/10.1271/bbb.60.2019
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- Matsui, T., Yoshimoto, C., Osajima, K., Oki, T., & Osajima, Y. (1996). In Vitro Survey of α-Glucosidase Inhibitory Food Components. Bioscience, Biotechnology, and Biochemistry. https://doi.org/10.1271/bbb.60.2019
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